Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28530

Experiment: WM_JK36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotides JDDGAC_28525: wzy - O157 family O-antigen polymerase, at 5,472,741 to 5,473,925 wzy JDDGAC_28530: wcaA - glycosyl transferase, at 5,473,894 to 5,474,640 wcaA JDDGAC_28535: wzx - O157 family O-antigen flippase, at 5,474,627 to 5,476,018 wzx Position (kb) 5473 5474 5475Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5473.094 kb on + strand, within wzyat 5473.120 kb on + strand, within wzyat 5473.336 kb on + strand, within wzyat 5473.336 kb on + strand, within wzyat 5473.841 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_JK36
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5,473,094 + wzy JDDGAC_28525 0.30 -0.5
5,473,120 + wzy JDDGAC_28525 0.32 -2.1
5,473,336 + wzy JDDGAC_28525 0.50 -4.8
5,473,336 + wzy JDDGAC_28525 0.50 -2.2
5,473,841 - -2.1

Or see this region's nucleotide sequence