Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28980

Experiment: Control_ECRC98

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_28965 and JDDGAC_28970 overlap by 4 nucleotidesJDDGAC_28970 and JDDGAC_28975 are separated by 462 nucleotidesJDDGAC_28975 and JDDGAC_28980 are separated by 41 nucleotidesJDDGAC_28980 and JDDGAC_28985 are separated by 291 nucleotidesJDDGAC_28985 and JDDGAC_28990 overlap by 4 nucleotidesJDDGAC_28990 and ymfN overlap by 4 nucleotides JDDGAC_28965: JDDGAC_28965 - DNA-binding protein, at 5,531,620 to 5,532,117 _28965 JDDGAC_28970: JDDGAC_28970 - DNA-binding protein, at 5,532,114 to 5,532,371 _28970 JDDGAC_28975: JDDGAC_28975 - DUF3950 domain-containing protein, at 5,532,834 to 5,533,061 _28975 JDDGAC_28980: JDDGAC_28980 - HNH endonuclease, at 5,533,103 to 5,533,468 _28980 JDDGAC_28985: JDDGAC_28985 - Putative terminase subunit encoded by prophage, at 5,533,760 to 5,534,059 _28985 JDDGAC_28990: JDDGAC_28990 - terminase small subunit, at 5,534,056 to 5,534,322 _28990 JDDGAC_28995: ymfN - terminase large subunit, at 5,534,319 to 5,534,951 ymfN Position (kb) 5533 5534Strain fitness (log2 ratio) -2 -1 0 1 2at 5532.270 kb on + strand, within JDDGAC_28970at 5532.369 kb on + strandat 5532.370 kb on - strandat 5532.389 kb on + strandat 5532.390 kb on - strandat 5532.413 kb on - strandat 5532.413 kb on - strandat 5532.413 kb on - strandat 5532.415 kb on - strandat 5533.400 kb on - strand, within JDDGAC_28980at 5533.400 kb on - strand, within JDDGAC_28980at 5534.078 kb on - strandat 5534.078 kb on - strandat 5534.080 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98
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5,532,270 + JDDGAC_28970 0.60 +0.2
5,532,369 + -0.6
5,532,370 - -1.0
5,532,389 + -0.9
5,532,390 - +0.5
5,532,413 - +0.6
5,532,413 - +0.1
5,532,413 - -1.5
5,532,415 - -2.4
5,533,400 - JDDGAC_28980 0.81 -2.0
5,533,400 - JDDGAC_28980 0.81 -0.3
5,534,078 - +1.9
5,534,078 - -0.2
5,534,080 - +0.8

Or see this region's nucleotide sequence