Strain Fitness in Escherichia coli ECRC98 around JDDGAC_21730

Experiment: Control_ECRC98

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfMet_trna and rimP are separated by 206 nucleotidesrimP and nusA are separated by 27 nucleotidesnusA and infB are separated by 24 nucleotides JDDGAC_21720: fMet_trna - tRNA-fMet(cat), at 4,157,296 to 4,157,372 _trna JDDGAC_21725: rimP - ribosome maturation factor RimP, at 4,157,579 to 4,158,031 rimP JDDGAC_21730: nusA - Transcription termination/antitermination protein NusA, at 4,158,059 to 4,159,546 nusA JDDGAC_21735: infB - Translation initiation factor IF-2, at 4,159,571 to 4,162,243 infB Position (kb) 4158 4159 4160Strain fitness (log2 ratio) -2 -1 0 1 2at 4157.067 kb on - strandat 4157.085 kb on + strandat 4157.085 kb on + strandat 4157.095 kb on - strandat 4157.198 kb on + strandat 4157.198 kb on + strandat 4157.199 kb on - strandat 4157.250 kb on - strandat 4157.250 kb on - strandat 4157.250 kb on - strandat 4157.250 kb on - strandat 4157.491 kb on + strandat 4159.377 kb on + strand, within nusAat 4159.377 kb on + strand, within nusAat 4159.420 kb on + strandat 4159.516 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98
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4,157,067 - +0.7
4,157,085 + -0.2
4,157,085 + +0.5
4,157,095 - -1.1
4,157,198 + +0.7
4,157,198 + +0.7
4,157,199 - +0.6
4,157,250 - +1.8
4,157,250 - -0.3
4,157,250 - +0.2
4,157,250 - -0.2
4,157,491 + +1.4
4,159,377 + nusA JDDGAC_21730 0.89 -1.1
4,159,377 + nusA JDDGAC_21730 0.89 -1.6
4,159,420 + -2.4
4,159,516 + -1.2

Or see this region's nucleotide sequence