Strain Fitness in Escherichia coli ECRC98 around JDDGAC_00260

Experiment: Control_ECRC98

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliP and fliO overlap by 1 nucleotidesfliO and fliN are separated by 2 nucleotidesfliN and fliM overlap by 4 nucleotides JDDGAC_00250: fliP - flagellar type III secretion system pore protein FliP, at 42,083 to 42,820 fliP JDDGAC_00255: fliO - flagellar type III secretion system protein FliO, at 42,820 to 43,185 fliO JDDGAC_00260: fliN - flagellar motor switch protein FliN, at 43,188 to 43,601 fliN JDDGAC_00265: fliM - flagellar motor switch protein FliM, at 43,598 to 44,602 fliM Position (kb) 43 44Strain fitness (log2 ratio) -2 -1 0 1 2 3at 42.300 kb on + strand, within fliPat 42.459 kb on - strand, within fliPat 42.521 kb on - strand, within fliPat 42.648 kb on - strand, within fliPat 42.810 kb on - strandat 42.840 kb on + strandat 43.180 kb on - strandat 43.224 kb on - strandat 43.224 kb on - strandat 43.249 kb on + strand, within fliNat 43.250 kb on - strand, within fliNat 43.287 kb on + strand, within fliNat 43.287 kb on + strand, within fliNat 43.424 kb on + strand, within fliNat 43.587 kb on - strandat 43.653 kb on - strandat 44.086 kb on + strand, within fliMat 44.108 kb on - strand, within fliMat 44.109 kb on + strand, within fliMat 44.367 kb on - strand, within fliMat 44.591 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98
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42,300 + fliP JDDGAC_00250 0.29 -0.2
42,459 - fliP JDDGAC_00250 0.51 +1.2
42,521 - fliP JDDGAC_00250 0.59 +1.4
42,648 - fliP JDDGAC_00250 0.77 -0.2
42,810 - -0.2
42,840 + +0.4
43,180 - -0.9
43,224 - +2.6
43,224 - +3.0
43,249 + fliN JDDGAC_00260 0.15 +0.0
43,250 - fliN JDDGAC_00260 0.15 -1.3
43,287 + fliN JDDGAC_00260 0.24 +0.2
43,287 + fliN JDDGAC_00260 0.24 -1.8
43,424 + fliN JDDGAC_00260 0.57 -1.0
43,587 - +1.7
43,653 - +1.8
44,086 + fliM JDDGAC_00265 0.49 -0.3
44,108 - fliM JDDGAC_00265 0.51 +0.1
44,109 + fliM JDDGAC_00265 0.51 -1.8
44,367 - fliM JDDGAC_00265 0.77 -0.6
44,591 - +0.3

Or see this region's nucleotide sequence