Strain Fitness in Escherichia coli ECRC98 around JDDGAC_25850

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfB and hyfA overlap by 1 nucleotideshyfA and bcp are separated by 252 nucleotidesbcp and gcvR overlap by 1 nucleotidesgcvR and dapA are separated by 145 nucleotides JDDGAC_25840: hyfB - hydrogenase 4 subunit B, at 4,939,916 to 4,941,934 hyfB JDDGAC_25845: hyfA - hydrogenase 4 subunit HyfA, at 4,941,934 to 4,942,551 hyfA JDDGAC_25850: bcp - thioredoxin-dependent thiol peroxidase, at 4,942,804 to 4,943,274 bcp JDDGAC_25855: gcvR - glycine cleavage system transcriptional repressor, at 4,943,274 to 4,943,846 gcvR JDDGAC_25860: dapA - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,943,992 to 4,944,870 dapA Position (kb) 4942 4943 4944Strain fitness (log2 ratio) -2 -1 0 1 2at 4941.838 kb on + strandat 4941.838 kb on + strandat 4941.842 kb on - strandat 4941.917 kb on + strandat 4941.918 kb on - strandat 4941.918 kb on - strandat 4942.459 kb on + strand, within hyfAat 4942.473 kb on + strand, within hyfAat 4942.506 kb on + strandat 4942.506 kb on + strandat 4942.506 kb on + strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.507 kb on - strandat 4942.751 kb on + strandat 4942.751 kb on + strandat 4942.752 kb on - strandat 4942.920 kb on + strand, within bcpat 4942.920 kb on + strand, within bcpat 4942.928 kb on + strand, within bcpat 4942.929 kb on - strand, within bcpat 4943.013 kb on + strand, within bcpat 4943.014 kb on - strand, within bcpat 4943.235 kb on + strandat 4943.235 kb on + strandat 4943.236 kb on - strandat 4943.276 kb on - strandat 4943.279 kb on + strandat 4943.279 kb on + strandat 4943.280 kb on - strandat 4943.280 kb on - strandat 4943.280 kb on - strandat 4943.308 kb on + strandat 4943.322 kb on - strandat 4943.348 kb on + strand, within gcvRat 4943.349 kb on - strand, within gcvRat 4943.349 kb on - strand, within gcvRat 4943.352 kb on + strand, within gcvRat 4943.375 kb on + strand, within gcvRat 4943.523 kb on + strand, within gcvRat 4943.664 kb on - strand, within gcvRat 4943.696 kb on + strand, within gcvRat 4943.697 kb on - strand, within gcvRat 4943.757 kb on - strand, within gcvRat 4943.825 kb on - strandat 4943.854 kb on - strandat 4943.905 kb on + strandat 4943.905 kb on + strandat 4943.906 kb on - strandat 4943.906 kb on - strandat 4943.956 kb on + strandat 4943.956 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,941,838 + -2.7
4,941,838 + +0.5
4,941,842 - -1.7
4,941,917 + -0.9
4,941,918 - +0.2
4,941,918 - -1.5
4,942,459 + hyfA JDDGAC_25845 0.85 -1.2
4,942,473 + hyfA JDDGAC_25845 0.87 -0.0
4,942,506 + +0.4
4,942,506 + -2.5
4,942,506 + -1.3
4,942,507 - -0.8
4,942,507 - -0.9
4,942,507 - -0.6
4,942,507 - +1.2
4,942,507 - -0.2
4,942,507 - -1.5
4,942,507 - -0.9
4,942,507 - -0.9
4,942,751 + +0.6
4,942,751 + -0.1
4,942,752 - -0.0
4,942,920 + bcp JDDGAC_25850 0.25 +0.9
4,942,920 + bcp JDDGAC_25850 0.25 -1.2
4,942,928 + bcp JDDGAC_25850 0.26 -1.3
4,942,929 - bcp JDDGAC_25850 0.27 +1.5
4,943,013 + bcp JDDGAC_25850 0.44 -1.0
4,943,014 - bcp JDDGAC_25850 0.45 -0.3
4,943,235 + +0.3
4,943,235 + +1.3
4,943,236 - -0.2
4,943,276 - +0.2
4,943,279 + +0.8
4,943,279 + -0.1
4,943,280 - +0.3
4,943,280 - -1.7
4,943,280 - -0.5
4,943,308 + -1.6
4,943,322 - -1.4
4,943,348 + gcvR JDDGAC_25855 0.13 -0.3
4,943,349 - gcvR JDDGAC_25855 0.13 -1.0
4,943,349 - gcvR JDDGAC_25855 0.13 +0.1
4,943,352 + gcvR JDDGAC_25855 0.14 +2.5
4,943,375 + gcvR JDDGAC_25855 0.18 -0.2
4,943,523 + gcvR JDDGAC_25855 0.43 -0.1
4,943,664 - gcvR JDDGAC_25855 0.68 -0.6
4,943,696 + gcvR JDDGAC_25855 0.74 +2.3
4,943,697 - gcvR JDDGAC_25855 0.74 -1.0
4,943,757 - gcvR JDDGAC_25855 0.84 +0.5
4,943,825 - -2.2
4,943,854 - -2.3
4,943,905 + -0.3
4,943,905 + -0.7
4,943,906 - -1.1
4,943,906 - -1.3
4,943,956 + -1.1
4,943,956 + +1.8

Or see this region's nucleotide sequence