Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgpB and ribA are separated by 169 nucleotides ribA and acnA are separated by 63 nucleotides acnA and ymiC are separated by 163 nucleotides ymiC and JDDGAC_05135 are separated by 128 nucleotides JDDGAC_05135 and ymiA are separated by 2 nucleotides
JDDGAC_05110: pgpB - phosphatidylglycerophosphatase B, at 897,108 to 897,872
pgpB
JDDGAC_05115: ribA - GTP cyclohydrolase-2, at 898,042 to 898,632
ribA
JDDGAC_05120: acnA - aconitate hydratase AcnA, at 898,696 to 901,371
acnA
JDDGAC_05130: ymiC - Protein YmiC, at 901,535 to 901,615
ymiC
JDDGAC_05135: JDDGAC_05135 - Protein, C-ter, truncated protein, at 901,744 to 901,911
_05135
JDDGAC_05140: ymiA - YmiA family putative membrane protein, at 901,914 to 902,042
ymiA
Position (kb) 898
899
900
901
902 Strain fitness (log2 ratio) -2
-1
0
1
2 at 897.708 kb on - strand, within pgpB at 897.759 kb on + strand, within pgpB at 897.760 kb on - strand, within pgpB at 897.821 kb on + strand at 897.831 kb on + strand at 898.005 kb on + strand at 898.005 kb on + strand at 898.005 kb on + strand at 899.058 kb on - strand, within acnA at 899.120 kb on + strand, within acnA at 899.125 kb on - strand, within acnA at 899.147 kb on - strand, within acnA at 899.256 kb on - strand, within acnA at 899.293 kb on + strand, within acnA at 899.294 kb on - strand, within acnA at 899.344 kb on - strand, within acnA at 899.457 kb on - strand, within acnA at 899.457 kb on - strand, within acnA at 899.481 kb on - strand, within acnA at 899.626 kb on + strand, within acnA at 899.641 kb on - strand, within acnA at 899.769 kb on + strand, within acnA at 899.868 kb on + strand, within acnA at 899.910 kb on - strand, within acnA at 900.205 kb on + strand, within acnA at 900.625 kb on + strand, within acnA at 900.902 kb on + strand, within acnA at 900.903 kb on - strand, within acnA at 900.903 kb on - strand, within acnA at 900.935 kb on - strand, within acnA at 901.206 kb on + strand at 901.304 kb on + strand at 901.304 kb on + strand at 901.305 kb on - strand at 901.344 kb on + strand at 901.631 kb on - strand at 901.631 kb on - strand at 901.636 kb on - strand at 901.654 kb on - strand at 901.654 kb on - strand at 901.681 kb on + strand at 901.746 kb on - strand at 901.746 kb on - strand at 901.805 kb on - strand, within JDDGAC_05135 at 901.805 kb on - strand, within JDDGAC_05135 at 901.867 kb on - strand, within JDDGAC_05135 at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.916 kb on - strand at 902.057 kb on - strand at 902.237 kb on - strand at 902.248 kb on + strand at 902.267 kb on - strand at 902.324 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 897,708 - pgpB JDDGAC_05110 0.78 +1.1 897,759 + pgpB JDDGAC_05110 0.85 +1.0 897,760 - pgpB JDDGAC_05110 0.85 -1.2 897,821 + -0.7 897,831 + -0.2 898,005 + -1.0 898,005 + +1.2 898,005 + +0.2 899,058 - acnA JDDGAC_05120 0.14 +0.8 899,120 + acnA JDDGAC_05120 0.16 -1.1 899,125 - acnA JDDGAC_05120 0.16 -1.2 899,147 - acnA JDDGAC_05120 0.17 -1.3 899,256 - acnA JDDGAC_05120 0.21 +0.2 899,293 + acnA JDDGAC_05120 0.22 +2.3 899,294 - acnA JDDGAC_05120 0.22 +1.0 899,344 - acnA JDDGAC_05120 0.24 -1.1 899,457 - acnA JDDGAC_05120 0.28 -0.1 899,457 - acnA JDDGAC_05120 0.28 -0.8 899,481 - acnA JDDGAC_05120 0.29 +0.2 899,626 + acnA JDDGAC_05120 0.35 -0.3 899,641 - acnA JDDGAC_05120 0.35 -0.4 899,769 + acnA JDDGAC_05120 0.40 -0.1 899,868 + acnA JDDGAC_05120 0.44 +0.7 899,910 - acnA JDDGAC_05120 0.45 -1.6 900,205 + acnA JDDGAC_05120 0.56 -1.4 900,625 + acnA JDDGAC_05120 0.72 -1.1 900,902 + acnA JDDGAC_05120 0.82 -0.6 900,903 - acnA JDDGAC_05120 0.82 +1.7 900,903 - acnA JDDGAC_05120 0.82 +2.0 900,935 - acnA JDDGAC_05120 0.84 +0.6 901,206 + -0.9 901,304 + -1.3 901,304 + +1.1 901,305 - -0.3 901,344 + +0.7 901,631 - +0.4 901,631 - +1.0 901,636 - -0.7 901,654 - +1.0 901,654 - -0.2 901,681 + +1.0 901,746 - +0.5 901,746 - -0.5 901,805 - JDDGAC_05135 0.36 -0.3 901,805 - JDDGAC_05135 0.36 +1.1 901,867 - JDDGAC_05135 0.73 -0.8 901,912 - -1.0 901,912 - -1.0 901,912 - -0.3 901,912 - +0.9 901,912 - +1.4 901,912 - -1.2 901,916 - +0.7 902,057 - -0.1 902,237 - -0.5 902,248 + +0.5 902,267 - -1.6 902,324 + -2.4
Or see this region's nucleotide sequence