Experiment: Control_ECRC98_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudB and JDDGAC_00830 are separated by 28 nucleotides JDDGAC_00830 and JDDGAC_00835 overlap by 1 nucleotides JDDGAC_00835 and ruvC overlap by 1 nucleotides ruvC and JDDGAC_00845 are separated by 1 nucleotides JDDGAC_00845 and yobI are separated by 70 nucleotides yobI and ruvA are separated by 138 nucleotides
JDDGAC_00825: nudB - dihydroneopterin triphosphate diphosphatase, at 138,620 to 139,129
nudB
JDDGAC_00830: JDDGAC_00830 - putative transcriptional regulatory protein YebC, at 139,158 to 139,898
_00830
JDDGAC_00835: JDDGAC_00835 - Crossover junction endodeoxyribonuclease RuvC, at 139,898 to 139,933
_00835
JDDGAC_00840: ruvC - crossover junction endodeoxyribonuclease RuvC, at 139,933 to 140,454
ruvC
JDDGAC_00845: JDDGAC_00845 - Protein, at 140,456 to 141,058
_00845
JDDGAC_00850: yobI - Uncharacterized protein YobI, at 141,129 to 141,194
yobI
JDDGAC_00855: ruvA - Holliday junction branch migration protein RuvA, at 141,333 to 141,944
ruvA
Position (kb) 139
140
141 Strain fitness (log2 ratio) -2
-1
0
1
2 at 138.936 kb on + strand, within nudB at 139.117 kb on + strand at 139.142 kb on - strand at 139.172 kb on + strand at 139.197 kb on - strand at 139.416 kb on + strand, within JDDGAC_00830 at 139.650 kb on + strand, within JDDGAC_00830 at 139.674 kb on + strand, within JDDGAC_00830 at 139.674 kb on + strand, within JDDGAC_00830 at 139.997 kb on + strand, within ruvC at 140.033 kb on + strand, within ruvC at 140.055 kb on - strand, within ruvC at 140.252 kb on + strand, within ruvC at 140.267 kb on + strand, within ruvC at 140.267 kb on + strand, within ruvC at 140.271 kb on + strand, within ruvC at 140.452 kb on + strand at 141.003 kb on - strand at 141.190 kb on + strand at 141.275 kb on + strand at 141.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC98_WM remove 138,936 + nudB JDDGAC_00825 0.62 -0.9 139,117 + +0.1 139,142 - -1.0 139,172 + +0.1 139,197 - +0.1 139,416 + JDDGAC_00830 0.35 +0.5 139,650 + JDDGAC_00830 0.66 -0.0 139,674 + JDDGAC_00830 0.70 +1.8 139,674 + JDDGAC_00830 0.70 +0.6 139,997 + ruvC JDDGAC_00840 0.12 -0.5 140,033 + ruvC JDDGAC_00840 0.19 -1.9 140,055 - ruvC JDDGAC_00840 0.23 -0.0 140,252 + ruvC JDDGAC_00840 0.61 -1.4 140,267 + ruvC JDDGAC_00840 0.64 -2.5 140,267 + ruvC JDDGAC_00840 0.64 +1.9 140,271 + ruvC JDDGAC_00840 0.65 -1.9 140,452 + -0.4 141,003 - -0.3 141,190 + -0.6 141,275 + -0.5 141,295 + +2.1
Or see this region's nucleotide sequence