Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_28890 and JDDGAC_28895 overlap by 10 nucleotides JDDGAC_28895 and Ile2_trna are separated by 313 nucleotides Ile2_trna and Arg_trna are separated by 1 nucleotides Arg_trna and Arg_trna are separated by 13 nucleotides Arg_trna and stxA2c are separated by 92 nucleotides
JDDGAC_28890: JDDGAC_28890 - Serine/threonine-protein phosphatase, at 5,523,724 to 5,524,395
_28890
JDDGAC_28895: JDDGAC_28895 - antiterminator, at 5,524,386 to 5,524,874
_28895
JDDGAC_28900: Ile2_trna - tRNA-Ile2(cat), at 5,525,188 to 5,525,263
_trna
JDDGAC_28905: Arg_trna - tRNA-Arg(tcg), at 5,525,265 to 5,525,341
_trna
JDDGAC_28910: Arg_trna - tRNA-Arg(tct), at 5,525,355 to 5,525,431
_trna
JDDGAC_28915: stxA2c - Shiga toxin Stx2c subunit A, at 5,525,524 to 5,526,483
stxA2c
Position (kb) 5525
5526 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5524.321 kb on + strand, within JDDGAC_28890 at 5524.394 kb on - strand at 5524.403 kb on - strand at 5524.502 kb on - strand, within JDDGAC_28895 at 5524.541 kb on + strand, within JDDGAC_28895 at 5524.541 kb on + strand, within JDDGAC_28895 at 5524.560 kb on - strand, within JDDGAC_28895 at 5524.608 kb on + strand, within JDDGAC_28895 at 5524.609 kb on - strand, within JDDGAC_28895 at 5524.644 kb on + strand, within JDDGAC_28895 at 5524.683 kb on + strand, within JDDGAC_28895 at 5524.686 kb on + strand, within JDDGAC_28895 at 5524.687 kb on - strand, within JDDGAC_28895 at 5524.687 kb on - strand, within JDDGAC_28895 at 5524.868 kb on - strand at 5524.872 kb on + strand at 5524.976 kb on + strand at 5525.078 kb on + strand at 5525.177 kb on - strand at 5525.286 kb on - strand, within Arg_trna at 5525.350 kb on - strand at 5525.363 kb on - strand, within Arg_trna at 5525.363 kb on - strand, within Arg_trna at 5525.534 kb on + strand at 5525.541 kb on + strand at 5525.585 kb on - strand at 5525.626 kb on + strand, within stxA2c at 5525.630 kb on + strand, within stxA2c at 5525.643 kb on + strand, within stxA2c at 5525.644 kb on - strand, within stxA2c at 5525.681 kb on + strand, within stxA2c at 5525.681 kb on + strand, within stxA2c at 5525.682 kb on - strand, within stxA2c at 5525.682 kb on - strand, within stxA2c at 5525.768 kb on - strand, within stxA2c at 5525.819 kb on - strand, within stxA2c at 5525.819 kb on - strand, within stxA2c at 5525.880 kb on + strand, within stxA2c at 5525.882 kb on + strand, within stxA2c at 5525.883 kb on - strand, within stxA2c at 5525.930 kb on - strand, within stxA2c at 5525.931 kb on + strand, within stxA2c at 5525.932 kb on - strand, within stxA2c at 5526.031 kb on - strand, within stxA2c at 5526.033 kb on + strand, within stxA2c at 5526.033 kb on + strand, within stxA2c at 5526.110 kb on + strand, within stxA2c at 5526.268 kb on - strand, within stxA2c at 5526.300 kb on + strand, within stxA2c
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 5,524,321 + JDDGAC_28890 0.89 -0.5 5,524,394 - +1.4 5,524,403 - -2.2 5,524,502 - JDDGAC_28895 0.24 -1.8 5,524,541 + JDDGAC_28895 0.32 +0.4 5,524,541 + JDDGAC_28895 0.32 -0.5 5,524,560 - JDDGAC_28895 0.36 -0.2 5,524,608 + JDDGAC_28895 0.45 -0.5 5,524,609 - JDDGAC_28895 0.46 +0.4 5,524,644 + JDDGAC_28895 0.53 -2.0 5,524,683 + JDDGAC_28895 0.61 +0.4 5,524,686 + JDDGAC_28895 0.61 -0.1 5,524,687 - JDDGAC_28895 0.62 -1.1 5,524,687 - JDDGAC_28895 0.62 +1.8 5,524,868 - -0.2 5,524,872 + -0.8 5,524,976 + -0.8 5,525,078 + -1.6 5,525,177 - -0.6 5,525,286 - Arg_trna JDDGAC_28905 0.27 -1.3 5,525,350 - -1.4 5,525,363 - Arg_trna JDDGAC_28910 0.10 -0.1 5,525,363 - Arg_trna JDDGAC_28910 0.10 -2.4 5,525,534 + +0.6 5,525,541 + +1.5 5,525,585 - -1.5 5,525,626 + stxA2c JDDGAC_28915 0.11 -2.7 5,525,630 + stxA2c JDDGAC_28915 0.11 +1.6 5,525,643 + stxA2c JDDGAC_28915 0.12 +0.7 5,525,644 - stxA2c JDDGAC_28915 0.12 -0.0 5,525,681 + stxA2c JDDGAC_28915 0.16 -1.0 5,525,681 + stxA2c JDDGAC_28915 0.16 +0.9 5,525,682 - stxA2c JDDGAC_28915 0.16 -0.5 5,525,682 - stxA2c JDDGAC_28915 0.16 -0.1 5,525,768 - stxA2c JDDGAC_28915 0.25 -0.1 5,525,819 - stxA2c JDDGAC_28915 0.31 +1.9 5,525,819 - stxA2c JDDGAC_28915 0.31 +1.1 5,525,880 + stxA2c JDDGAC_28915 0.37 +1.5 5,525,882 + stxA2c JDDGAC_28915 0.37 +1.4 5,525,883 - stxA2c JDDGAC_28915 0.37 -1.2 5,525,930 - stxA2c JDDGAC_28915 0.42 -1.0 5,525,931 + stxA2c JDDGAC_28915 0.42 -0.3 5,525,932 - stxA2c JDDGAC_28915 0.42 -1.2 5,526,031 - stxA2c JDDGAC_28915 0.53 +0.5 5,526,033 + stxA2c JDDGAC_28915 0.53 -0.2 5,526,033 + stxA2c JDDGAC_28915 0.53 -1.6 5,526,110 + stxA2c JDDGAC_28915 0.61 +0.9 5,526,268 - stxA2c JDDGAC_28915 0.78 +1.2 5,526,300 + stxA2c JDDGAC_28915 0.81 -0.0
Or see this region's nucleotide sequence