Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_23420 and fliN overlap by 41 nucleotides fliN and escR overlap by 11 nucleotides escR and JDDGAC_23435 are separated by 10 nucleotides JDDGAC_23435 and sctT are separated by 1 nucleotides sctT and sctT are separated by 78 nucleotides
JDDGAC_23420: JDDGAC_23420 - type III secretion protein, at 4,490,816 to 4,491,385
_23420
JDDGAC_23425: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 4,491,345 to 4,492,331
fliN
JDDGAC_23430: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 4,492,321 to 4,492,986
escR
JDDGAC_23435: JDDGAC_23435 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 4,492,997 to 4,493,257
_23435
JDDGAC_23440: sctT - type III secretion system export apparatus subunit SctT, at 4,493,259 to 4,493,711
sctT
JDDGAC_23445: sctT - type III secretion system export apparatus subunit SctT, at 4,493,790 to 4,494,026
sctT
Position (kb) 4492
4493 Strain fitness (log2 ratio) -2
-1
0
1 at 4491.776 kb on + strand, within fliN at 4492.656 kb on + strand, within escR at 4492.853 kb on + strand, within escR at 4492.884 kb on - strand, within escR at 4492.955 kb on + strand at 4493.024 kb on - strand, within JDDGAC_23435 at 4493.043 kb on + strand, within JDDGAC_23435 at 4493.272 kb on + strand at 4493.289 kb on - strand at 4493.293 kb on + strand at 4493.304 kb on - strand at 4493.522 kb on - strand, within sctT at 4493.549 kb on + strand, within sctT at 4493.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 4,491,776 + fliN JDDGAC_23425 0.44 +0.2 4,492,656 + escR JDDGAC_23430 0.50 -0.7 4,492,853 + escR JDDGAC_23430 0.80 -2.5 4,492,884 - escR JDDGAC_23430 0.85 -0.9 4,492,955 + +0.8 4,493,024 - JDDGAC_23435 0.10 -1.1 4,493,043 + JDDGAC_23435 0.18 -1.0 4,493,272 + -0.6 4,493,289 - +0.0 4,493,293 + -0.0 4,493,304 - -0.6 4,493,522 - sctT JDDGAC_23440 0.58 +0.4 4,493,549 + sctT JDDGAC_23440 0.64 +0.6 4,493,808 + +0.1
Or see this region's nucleotide sequence