Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19320

Experiment: WM_Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaL and rfaC overlap by 4 nucleotidesrfaC and rfaF are separated by 3 nucleotidesrfaF and rfaD are separated by 9 nucleotides JDDGAC_19310: rfaL - O-antigen ligase RfaL, at 3,673,000 to 3,674,208 rfaL JDDGAC_19315: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 3,674,205 to 3,675,197 rfaC JDDGAC_19320: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 3,675,201 to 3,676,247 rfaF JDDGAC_19325: rfaD - ADP-glyceromanno-heptose 6-epimerase, at 3,676,257 to 3,677,189 rfaD Position (kb) 3675 3676 3677Strain fitness (log2 ratio) -1 0 1at 3674.239 kb on - strandat 3675.217 kb on - strandat 3675.217 kb on - strandat 3675.223 kb on - strandat 3675.389 kb on - strand, within rfaFat 3675.743 kb on - strand, within rfaFat 3675.830 kb on - strand, within rfaFat 3675.889 kb on - strand, within rfaFat 3675.889 kb on - strand, within rfaFat 3675.988 kb on - strand, within rfaFat 3676.742 kb on - strand, within rfaDat 3677.190 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas58
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3,674,239 - +1.1
3,675,217 - -0.4
3,675,217 - -1.1
3,675,223 - +0.8
3,675,389 - rfaF JDDGAC_19320 0.18 +0.7
3,675,743 - rfaF JDDGAC_19320 0.52 -0.8
3,675,830 - rfaF JDDGAC_19320 0.60 +1.7
3,675,889 - rfaF JDDGAC_19320 0.66 -0.3
3,675,889 - rfaF JDDGAC_19320 0.66 -0.1
3,675,988 - rfaF JDDGAC_19320 0.75 +0.7
3,676,742 - rfaD JDDGAC_19325 0.52 +1.7
3,677,190 - -0.3

Or see this region's nucleotide sequence