Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phrB and ybgA overlap by 4 nucleotides ybgA and yafF are separated by 148 nucleotides
JDDGAC_10680: phrB - deoxyribodipyrimidine photo-lyase, at 1,907,773 to 1,909,191
phrB
JDDGAC_10685: ybgA - DUF1722 domain-containing protein, at 1,909,188 to 1,909,697
ybgA
JDDGAC_10690: yafF - ISAs1 family transposase, at 1,909,846 to 1,910,982
yafF
Position (kb)
1909
1910
1911 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1908.876 kb on - strand, within phrB at 1908.881 kb on + strand, within phrB at 1908.882 kb on - strand, within phrB at 1908.882 kb on - strand, within phrB at 1908.882 kb on - strand, within phrB at 1908.882 kb on - strand, within phrB at 1908.885 kb on + strand, within phrB at 1909.053 kb on + strand at 1909.053 kb on + strand at 1909.054 kb on - strand at 1909.057 kb on - strand at 1909.088 kb on + strand at 1909.088 kb on + strand at 1909.089 kb on - strand at 1909.143 kb on + strand at 1909.156 kb on + strand at 1909.156 kb on + strand at 1909.157 kb on - strand at 1909.191 kb on + strand at 1909.192 kb on - strand at 1909.214 kb on + strand at 1909.240 kb on + strand, within ybgA at 1909.264 kb on + strand, within ybgA at 1909.280 kb on + strand, within ybgA at 1909.280 kb on + strand, within ybgA at 1909.281 kb on - strand, within ybgA at 1909.282 kb on + strand, within ybgA at 1909.283 kb on - strand, within ybgA at 1909.283 kb on - strand, within ybgA at 1909.283 kb on - strand, within ybgA at 1909.283 kb on - strand, within ybgA at 1909.283 kb on - strand, within ybgA at 1909.579 kb on + strand, within ybgA at 1909.724 kb on + strand at 1909.731 kb on - strand at 1909.740 kb on + strand at 1909.759 kb on + strand at 1909.774 kb on - strand at 1909.785 kb on - strand at 1909.786 kb on + strand at 1909.811 kb on - strand at 1910.527 kb on - strand, within yafF at 1911.650 kb on - strand at 1911.794 kb on + strand at 1911.806 kb on + strand at 1911.863 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 1,908,876 - phrB JDDGAC_10680 0.78 +0.6 1,908,881 + phrB JDDGAC_10680 0.78 +0.4 1,908,882 - phrB JDDGAC_10680 0.78 +0.1 1,908,882 - phrB JDDGAC_10680 0.78 +1.3 1,908,882 - phrB JDDGAC_10680 0.78 -1.2 1,908,882 - phrB JDDGAC_10680 0.78 +0.5 1,908,885 + phrB JDDGAC_10680 0.78 +2.2 1,909,053 + -0.1 1,909,053 + +0.4 1,909,054 - -0.3 1,909,057 - +0.1 1,909,088 + +0.5 1,909,088 + +0.7 1,909,089 - +1.6 1,909,143 + -1.0 1,909,156 + +0.1 1,909,156 + +2.1 1,909,157 - -1.4 1,909,191 + +0.5 1,909,192 - -0.1 1,909,214 + -1.3 1,909,240 + ybgA JDDGAC_10685 0.10 +1.6 1,909,264 + ybgA JDDGAC_10685 0.15 +0.7 1,909,280 + ybgA JDDGAC_10685 0.18 +0.0 1,909,280 + ybgA JDDGAC_10685 0.18 -2.3 1,909,281 - ybgA JDDGAC_10685 0.18 +1.1 1,909,282 + ybgA JDDGAC_10685 0.18 -0.3 1,909,283 - ybgA JDDGAC_10685 0.19 +0.4 1,909,283 - ybgA JDDGAC_10685 0.19 +0.1 1,909,283 - ybgA JDDGAC_10685 0.19 -1.0 1,909,283 - ybgA JDDGAC_10685 0.19 -0.6 1,909,283 - ybgA JDDGAC_10685 0.19 +0.2 1,909,579 + ybgA JDDGAC_10685 0.77 +0.8 1,909,724 + -2.3 1,909,731 - -1.1 1,909,740 + -0.3 1,909,759 + -0.4 1,909,774 - -1.4 1,909,785 - -0.8 1,909,786 + +1.4 1,909,811 - -1.3 1,910,527 - yafF JDDGAC_10690 0.60 -1.4 1,911,650 - -0.4 1,911,794 + -1.8 1,911,806 + -1.3 1,911,863 + -0.2
Or see this region's nucleotide sequence