Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaA and Ser_trna are separated by 425 nucleotides Ser_trna and JDDGAC_08430 are separated by 104 nucleotides
JDDGAC_08420: hyaA - hydrogenase 1 small subunit, at 1,481,666 to 1,482,784
hyaA
JDDGAC_08425: Ser_trna - tRNA-Ser(tga), at 1,483,210 to 1,483,297
_trna
JDDGAC_08430: JDDGAC_08430 - hypothetical protein, at 1,483,402 to 1,483,584
_08430
Position (kb)
1483
1484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1482.425 kb on + strand, within hyaA at 1482.425 kb on + strand, within hyaA at 1482.425 kb on + strand, within hyaA at 1482.438 kb on + strand, within hyaA at 1482.439 kb on - strand, within hyaA at 1482.439 kb on - strand, within hyaA at 1482.439 kb on - strand, within hyaA at 1482.451 kb on - strand, within hyaA at 1482.451 kb on - strand, within hyaA at 1482.451 kb on - strand, within hyaA at 1482.451 kb on - strand, within hyaA at 1482.684 kb on - strand at 1482.709 kb on + strand at 1482.831 kb on + strand at 1483.000 kb on - strand at 1483.006 kb on - strand at 1483.068 kb on - strand at 1483.121 kb on + strand at 1483.300 kb on + strand at 1483.361 kb on - strand at 1483.510 kb on + strand, within JDDGAC_08430 at 1483.540 kb on + strand, within JDDGAC_08430 at 1483.560 kb on + strand, within JDDGAC_08430 at 1483.635 kb on + strand at 1483.650 kb on + strand at 1483.740 kb on + strand at 1483.741 kb on - strand at 1483.898 kb on - strand at 1483.943 kb on + strand at 1483.955 kb on + strand at 1483.956 kb on - strand at 1483.956 kb on - strand at 1483.957 kb on + strand at 1483.957 kb on + strand at 1484.060 kb on + strand at 1484.061 kb on - strand at 1484.085 kb on - strand at 1484.115 kb on + strand at 1484.116 kb on - strand at 1484.118 kb on - strand at 1484.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 1,482,425 + hyaA JDDGAC_08420 0.68 -0.2 1,482,425 + hyaA JDDGAC_08420 0.68 -0.6 1,482,425 + hyaA JDDGAC_08420 0.68 -0.1 1,482,438 + hyaA JDDGAC_08420 0.69 -0.5 1,482,439 - hyaA JDDGAC_08420 0.69 +0.7 1,482,439 - hyaA JDDGAC_08420 0.69 -0.3 1,482,439 - hyaA JDDGAC_08420 0.69 +0.5 1,482,451 - hyaA JDDGAC_08420 0.70 -3.0 1,482,451 - hyaA JDDGAC_08420 0.70 +0.0 1,482,451 - hyaA JDDGAC_08420 0.70 -0.2 1,482,451 - hyaA JDDGAC_08420 0.70 -2.3 1,482,684 - -0.3 1,482,709 + +1.0 1,482,831 + -0.2 1,483,000 - +0.7 1,483,006 - +1.4 1,483,068 - +0.1 1,483,121 + -1.2 1,483,300 + +0.5 1,483,361 - +3.3 1,483,510 + JDDGAC_08430 0.59 -1.2 1,483,540 + JDDGAC_08430 0.75 -0.9 1,483,560 + JDDGAC_08430 0.86 -3.0 1,483,635 + -0.3 1,483,650 + +0.4 1,483,740 + +1.7 1,483,741 - +1.3 1,483,898 - +0.9 1,483,943 + +1.3 1,483,955 + -2.2 1,483,956 - +1.7 1,483,956 - +0.3 1,483,957 + +0.2 1,483,957 + +0.8 1,484,060 + +0.2 1,484,061 - +0.9 1,484,085 - -0.8 1,484,115 + -0.2 1,484,116 - +1.4 1,484,118 - +1.3 1,484,168 - -0.1
Or see this region's nucleotide sequence