Strain Fitness in Escherichia coli ECRC98 around JDDGAC_06130

Experiment: WM_Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfA and hemA are separated by 41 nucleotideshemA and lolB are separated by 213 nucleotideslolB and ispE overlap by 1 nucleotides JDDGAC_06125: prfA - peptide chain release factor 1, at 1,065,572 to 1,066,654 prfA JDDGAC_06130: hemA - glutamyl-tRNA reductase, at 1,066,696 to 1,067,952 hemA JDDGAC_06135: lolB - lipoprotein insertase outer membrane protein LolB, at 1,068,166 to 1,068,789 lolB JDDGAC_06140: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 1,068,789 to 1,069,640 ispE Position (kb) 1066 1067 1068Strain fitness (log2 ratio) -3 -2 -1 0 1at 1066.052 kb on - strand, within prfAat 1066.141 kb on - strand, within prfAat 1066.692 kb on - strandat 1066.692 kb on - strandat 1066.692 kb on - strandat 1066.692 kb on - strandat 1067.011 kb on - strand, within hemAat 1067.787 kb on - strand, within hemAat 1068.123 kb on + strandat 1068.132 kb on + strandat 1068.343 kb on + strand, within lolBat 1068.650 kb on - strand, within lolB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas58
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1,066,052 - prfA JDDGAC_06125 0.44 -2.6
1,066,141 - prfA JDDGAC_06125 0.53 -1.8
1,066,692 - +1.4
1,066,692 - -1.0
1,066,692 - +0.8
1,066,692 - +0.9
1,067,011 - hemA JDDGAC_06130 0.25 -1.7
1,067,787 - hemA JDDGAC_06130 0.87 -2.3
1,068,123 + -0.5
1,068,132 + -0.4
1,068,343 + lolB JDDGAC_06135 0.28 -1.2
1,068,650 - lolB JDDGAC_06135 0.78 -3.4

Or see this region's nucleotide sequence