Strain Fitness in Escherichia coli ECRC98 around JDDGAC_04935

Experiment: WM_Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjM and JDDGAC_04930 overlap by 1 nucleotidesJDDGAC_04930 and pspE are separated by 209 nucleotidespspE and pspD are separated by 74 nucleotidespspD and pspC are separated by 8 nucleotidespspC and pspB overlap by 1 nucleotidespspB and pspA are separated by 53 nucleotides JDDGAC_04925: ycjM - Glucosylglycerate phosphorylase, at 858,205 to 859,509 ycjM JDDGAC_04930: JDDGAC_04930 - Alpha-amylase, at 859,509 to 859,883 _04930 JDDGAC_04935: pspE - thiosulfate sulfurtransferase PspE, at 860,093 to 860,407 pspE JDDGAC_04940: pspD - phage shock protein PspD, at 860,482 to 860,703 pspD JDDGAC_04945: pspC - envelope stress response membrane protein PspC, at 860,712 to 861,071 pspC JDDGAC_04950: pspB - envelope stress response membrane protein PspB, at 861,071 to 861,295 pspB JDDGAC_04955: pspA - phage shock protein PspA, at 861,349 to 862,017 pspA Position (kb) 860 861Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 859.185 kb on + strand, within ycjMat 860.022 kb on + strandat 860.022 kb on + strandat 860.155 kb on - strand, within pspEat 860.282 kb on - strand, within pspEat 860.314 kb on - strand, within pspEat 860.410 kb on - strandat 860.410 kb on - strandat 860.421 kb on + strandat 860.509 kb on - strand, within pspDat 860.703 kb on + strandat 860.761 kb on + strand, within pspCat 860.762 kb on - strand, within pspCat 860.762 kb on - strand, within pspCat 860.902 kb on + strand, within pspCat 860.902 kb on + strand, within pspCat 860.903 kb on - strand, within pspCat 860.903 kb on - strand, within pspCat 860.977 kb on - strand, within pspCat 860.987 kb on - strand, within pspCat 860.987 kb on - strand, within pspCat 861.295 kb on + strandat 861.300 kb on - strandat 861.351 kb on - strandat 861.351 kb on - strandat 861.380 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas58
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859,185 + ycjM JDDGAC_04925 0.75 -1.3
860,022 + +2.4
860,022 + -0.0
860,155 - pspE JDDGAC_04935 0.20 -0.3
860,282 - pspE JDDGAC_04935 0.60 -2.1
860,314 - pspE JDDGAC_04935 0.70 -1.6
860,410 - -2.1
860,410 - +0.6
860,421 + +1.1
860,509 - pspD JDDGAC_04940 0.12 +1.1
860,703 + -0.4
860,761 + pspC JDDGAC_04945 0.14 -0.6
860,762 - pspC JDDGAC_04945 0.14 +0.3
860,762 - pspC JDDGAC_04945 0.14 +1.2
860,902 + pspC JDDGAC_04945 0.53 +0.4
860,902 + pspC JDDGAC_04945 0.53 +0.7
860,903 - pspC JDDGAC_04945 0.53 +0.8
860,903 - pspC JDDGAC_04945 0.53 +0.1
860,977 - pspC JDDGAC_04945 0.74 -1.1
860,987 - pspC JDDGAC_04945 0.76 -0.3
860,987 - pspC JDDGAC_04945 0.76 +0.1
861,295 + +0.9
861,300 - +0.0
861,351 - -0.8
861,351 - -3.3
861,380 - -0.9

Or see this region's nucleotide sequence