Strain Fitness in Escherichia coli ECRC98 around JDDGAC_03775

Experiment: WM_Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpqqL and gadB are separated by 361 nucleotidesgadB and gadC are separated by 155 nucleotides JDDGAC_03770: pqqL - putative zinc protease PqqL, at 642,761 to 645,544 pqqL JDDGAC_03775: gadB - glutamate decarboxylase, at 645,906 to 647,306 gadB JDDGAC_03780: gadC - acid resistance gamma-aminobutyrate antiporter GadC, at 647,462 to 648,997 gadC Position (kb) 645 646 647 648Strain fitness (log2 ratio) -2 -1 0 1 2at 644.915 kb on + strand, within pqqLat 645.224 kb on - strand, within pqqLat 645.389 kb on - strandat 645.848 kb on + strandat 645.971 kb on + strandat 646.138 kb on + strand, within gadBat 646.992 kb on + strand, within gadBat 646.992 kb on + strand, within gadBat 647.273 kb on + strandat 647.300 kb on + strandat 647.300 kb on + strandat 647.442 kb on + strandat 647.467 kb on + strandat 647.472 kb on + strandat 647.704 kb on + strand, within gadCat 647.969 kb on - strand, within gadCat 648.103 kb on + strand, within gadCat 648.104 kb on - strand, within gadCat 648.173 kb on - strand, within gadC

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas58
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644,915 + pqqL JDDGAC_03770 0.77 -1.1
645,224 - pqqL JDDGAC_03770 0.88 -2.0
645,389 - +1.0
645,848 + -2.3
645,971 + -2.1
646,138 + gadB JDDGAC_03775 0.17 -2.4
646,992 + gadB JDDGAC_03775 0.78 -0.7
646,992 + gadB JDDGAC_03775 0.78 -2.3
647,273 + -0.3
647,300 + -1.3
647,300 + +0.4
647,442 + -1.7
647,467 + -2.3
647,472 + -2.4
647,704 + gadC JDDGAC_03780 0.16 +2.0
647,969 - gadC JDDGAC_03780 0.33 +0.5
648,103 + gadC JDDGAC_03780 0.42 -1.4
648,104 - gadC JDDGAC_03780 0.42 +1.0
648,173 - gadC JDDGAC_03780 0.46 -2.0

Or see this region's nucleotide sequence