Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and tnp-IS3 overlap by 1 nucleotides tnp-IS3 and JDDGAC_01920 are separated by 2 nucleotides JDDGAC_01920 and ydhR are separated by 125 nucleotides ydhR and Val_trna are separated by 107 nucleotides Val_trna and Val_trna are separated by 4 nucleotides
JDDGAC_01910: tnp-IS3 - IS3 family transposase, at 341,992 to 342,318
tnp-IS3
JDDGAC_01915: tnp-IS3 - IS3 family transposase, at 342,318 to 343,205
tnp-IS3
JDDGAC_01920: JDDGAC_01920 - FAD/NAD(P)-binding protein, at 343,208 to 343,711
_01920
JDDGAC_01925: ydhR - monooxygenase, at 343,837 to 344,142
ydhR
JDDGAC_01935: Val_trna - tRNA-Val(gac), at 344,250 to 344,326
_trna
JDDGAC_01940: Val_trna - tRNA-Val(gac), at 344,331 to 344,407
_trna
Position (kb) 343
344 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 342.757 kb on - strand, within tnp-IS3 at 343.373 kb on + strand, within JDDGAC_01920 at 343.515 kb on + strand, within JDDGAC_01920 at 343.627 kb on + strand, within JDDGAC_01920 at 343.720 kb on - strand at 343.757 kb on - strand at 343.983 kb on - strand, within ydhR at 344.570 kb on + strand at 344.659 kb on + strand at 344.667 kb on - strand at 344.672 kb on + strand at 344.672 kb on + strand at 344.673 kb on - strand at 344.673 kb on - strand at 344.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 342,757 - tnp-IS3 JDDGAC_01915 0.49 -1.7 343,373 + JDDGAC_01920 0.33 -0.7 343,515 + JDDGAC_01920 0.61 -1.5 343,627 + JDDGAC_01920 0.83 -0.2 343,720 - +1.6 343,757 - -1.1 343,983 - ydhR JDDGAC_01925 0.48 +0.3 344,570 + +2.1 344,659 + +0.6 344,667 - -1.7 344,672 + +2.2 344,672 + -0.9 344,673 - +0.5 344,673 - -1.3 344,682 + -2.8
Or see this region's nucleotide sequence