Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and ynjH are separated by 235 nucleotides ynjH and nudG overlap by 35 nucleotides nudG and ynjF are separated by 86 nucleotides ynjF and ynjE are separated by 8 nucleotides
JDDGAC_01415: gdhA - NADP-specific glutamate dehydrogenase, at 246,013 to 247,356
gdhA
JDDGAC_01420: ynjH - Uncharacterized protein YnjH, at 247,592 to 247,864
ynjH
JDDGAC_01425: nudG - CTP pyrophosphohydrolase, at 247,830 to 248,237
nudG
JDDGAC_01430: ynjF - Inner membrane protein YnjF, at 248,324 to 248,944
ynjF
JDDGAC_01435: ynjE - thiosulfate sulfurtransferase YnjE, at 248,953 to 250,260
ynjE
Position (kb) 247
248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 246.997 kb on - strand, within gdhA at 247.264 kb on + strand at 247.265 kb on - strand at 247.363 kb on - strand at 247.373 kb on + strand at 247.373 kb on + strand at 247.375 kb on + strand at 247.376 kb on - strand at 247.403 kb on - strand at 247.403 kb on - strand at 247.430 kb on + strand at 247.432 kb on + strand at 247.433 kb on - strand at 247.453 kb on - strand at 247.486 kb on - strand at 247.562 kb on - strand at 247.633 kb on - strand, within ynjH at 247.674 kb on + strand, within ynjH at 247.674 kb on + strand, within ynjH at 247.675 kb on - strand, within ynjH at 247.778 kb on - strand, within ynjH at 247.791 kb on + strand, within ynjH at 247.831 kb on + strand at 247.831 kb on + strand at 248.042 kb on - strand, within nudG at 248.042 kb on - strand, within nudG at 248.127 kb on - strand, within nudG at 248.275 kb on + strand at 248.306 kb on + strand at 248.328 kb on + strand at 248.623 kb on + strand, within ynjF at 248.768 kb on + strand, within ynjF at 248.771 kb on + strand, within ynjF at 248.808 kb on + strand, within ynjF at 248.955 kb on - strand at 248.955 kb on - strand at 249.071 kb on + strand at 249.110 kb on + strand, within ynjE at 249.138 kb on + strand, within ynjE at 249.157 kb on - strand, within ynjE at 249.165 kb on + strand, within ynjE
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 246,997 - gdhA JDDGAC_01415 0.73 +0.5 247,264 + -0.9 247,265 - +0.9 247,363 - -1.2 247,373 + +1.2 247,373 + +0.8 247,375 + -0.1 247,376 - -0.3 247,403 - -0.4 247,403 - +0.5 247,430 + -1.2 247,432 + +0.6 247,433 - +0.6 247,453 - +0.8 247,486 - +0.3 247,562 - -1.5 247,633 - ynjH JDDGAC_01420 0.15 +0.2 247,674 + ynjH JDDGAC_01420 0.30 -1.7 247,674 + ynjH JDDGAC_01420 0.30 -1.7 247,675 - ynjH JDDGAC_01420 0.30 +0.2 247,778 - ynjH JDDGAC_01420 0.68 -0.6 247,791 + ynjH JDDGAC_01420 0.73 -0.4 247,831 + +1.0 247,831 + -0.9 248,042 - nudG JDDGAC_01425 0.52 -2.0 248,042 - nudG JDDGAC_01425 0.52 -1.2 248,127 - nudG JDDGAC_01425 0.73 +0.1 248,275 + -0.0 248,306 + +0.4 248,328 + -1.3 248,623 + ynjF JDDGAC_01430 0.48 +0.3 248,768 + ynjF JDDGAC_01430 0.71 +0.8 248,771 + ynjF JDDGAC_01430 0.72 -0.2 248,808 + ynjF JDDGAC_01430 0.78 +0.4 248,955 - +0.3 248,955 - -0.4 249,071 + +0.5 249,110 + ynjE JDDGAC_01435 0.12 -0.7 249,138 + ynjE JDDGAC_01435 0.14 -2.8 249,157 - ynjE JDDGAC_01435 0.16 -0.6 249,165 + ynjE JDDGAC_01435 0.16 +1.9
Or see this region's nucleotide sequence