Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt beeE and JDDGAC_29030 overlap by 4 nucleotides JDDGAC_29030 and JDDGAC_29035 overlap by 4 nucleotides JDDGAC_29035 and JDDGAC_29040 are separated by 9 nucleotides JDDGAC_29040 and JDDGAC_29045 overlap by 4 nucleotides
JDDGAC_29025: beeE - phage portal protein, at 5,539,506 to 5,540,870
beeE
JDDGAC_29030: JDDGAC_29030 - Putative prophage portal protein, at 5,540,867 to 5,542,090
_29030
JDDGAC_29035: JDDGAC_29035 - head-tail connector protein, at 5,542,087 to 5,542,413
_29035
JDDGAC_29040: JDDGAC_29040 - phage head closure protein, at 5,542,423 to 5,542,773
_29040
JDDGAC_29045: JDDGAC_29045 - phage portal protein, at 5,542,770 to 5,543,216
_29045
Position (kb) 5540
5541
5542
5543 Strain fitness (log2 ratio) -2
-1
0
1 at 5539.914 kb on - strand, within beeE at 5539.914 kb on - strand, within beeE at 5540.088 kb on + strand, within beeE at 5540.123 kb on + strand, within beeE at 5540.244 kb on + strand, within beeE at 5540.246 kb on + strand, within beeE at 5540.246 kb on + strand, within beeE at 5540.391 kb on + strand, within beeE at 5541.920 kb on + strand, within JDDGAC_29030 at 5541.964 kb on + strand, within JDDGAC_29030 at 5542.004 kb on - strand at 5542.222 kb on - strand, within JDDGAC_29035 at 5542.457 kb on + strand at 5542.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 5,539,914 - beeE JDDGAC_29025 0.30 +0.2 5,539,914 - beeE JDDGAC_29025 0.30 +0.9 5,540,088 + beeE JDDGAC_29025 0.43 -0.4 5,540,123 + beeE JDDGAC_29025 0.45 +0.5 5,540,244 + beeE JDDGAC_29025 0.54 +0.5 5,540,246 + beeE JDDGAC_29025 0.54 +1.1 5,540,246 + beeE JDDGAC_29025 0.54 +0.4 5,540,391 + beeE JDDGAC_29025 0.65 -0.1 5,541,920 + JDDGAC_29030 0.86 -0.6 5,541,964 + JDDGAC_29030 0.90 -1.9 5,542,004 - +1.2 5,542,222 - JDDGAC_29035 0.41 +0.3 5,542,457 + -2.2 5,542,457 + +0.5
Or see this region's nucleotide sequence