Strain Fitness in Escherichia coli ECRC98 around JDDGAC_27715

Experiment: WM_Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_27700 and JDDGAC_27705 overlap by 28 nucleotidesJDDGAC_27705 and exoD overlap by 1 nucleotidesexoD and bet overlap by 4 nucleotidesbet and JDDGAC_27720 are separated by 5 nucleotidesJDDGAC_27720 and JDDGAC_27725 are separated by 75 nucleotides JDDGAC_27700: JDDGAC_27700 - DUF1382 domain-containing protein, at 5,321,939 to 5,322,130 _27700 JDDGAC_27705: JDDGAC_27705 - cruciferin, at 5,322,103 to 5,322,285 _27705 JDDGAC_27710: exoD - Putative uncharacterized protein ExoD, at 5,322,285 to 5,322,962 exoD JDDGAC_27715: bet - phage recombination protein Bet, at 5,322,959 to 5,323,744 bet JDDGAC_27720: JDDGAC_27720 - host-nuclease inhibitor protein Gam, at 5,323,750 to 5,324,046 _27720 JDDGAC_27725: JDDGAC_27725 - cell division inhibitor protein, at 5,324,122 to 5,324,328 _27725 Position (kb) 5322 5323 5324Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5322.130 kb on + strandat 5322.147 kb on + strand, within JDDGAC_27705at 5322.148 kb on - strand, within JDDGAC_27705at 5322.286 kb on + strandat 5322.304 kb on - strandat 5322.304 kb on - strandat 5322.304 kb on - strandat 5322.407 kb on - strand, within exoDat 5322.452 kb on - strand, within exoDat 5322.843 kb on + strand, within exoDat 5322.844 kb on - strand, within exoDat 5322.844 kb on - strand, within exoDat 5323.195 kb on - strand, within betat 5323.236 kb on + strand, within betat 5323.293 kb on - strand, within betat 5323.293 kb on - strand, within betat 5324.033 kb on + strandat 5324.033 kb on + strandat 5324.036 kb on + strandat 5324.102 kb on + strandat 5324.183 kb on + strand, within JDDGAC_27725at 5324.183 kb on + strand, within JDDGAC_27725at 5324.184 kb on - strand, within JDDGAC_27725at 5324.229 kb on + strand, within JDDGAC_27725at 5324.363 kb on + strandat 5324.365 kb on + strandat 5324.416 kb on + strandat 5324.417 kb on - strandat 5324.661 kb on + strandat 5324.662 kb on - strandat 5324.683 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas58
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5,322,130 + -0.7
5,322,147 + JDDGAC_27705 0.24 -1.6
5,322,148 - JDDGAC_27705 0.25 +1.4
5,322,286 + -1.8
5,322,304 - -4.1
5,322,304 - +0.7
5,322,304 - -0.8
5,322,407 - exoD JDDGAC_27710 0.18 -0.7
5,322,452 - exoD JDDGAC_27710 0.25 +1.5
5,322,843 + exoD JDDGAC_27710 0.82 +2.7
5,322,844 - exoD JDDGAC_27710 0.82 +1.1
5,322,844 - exoD JDDGAC_27710 0.82 -0.5
5,323,195 - bet JDDGAC_27715 0.30 +1.4
5,323,236 + bet JDDGAC_27715 0.35 -2.1
5,323,293 - bet JDDGAC_27715 0.42 -0.7
5,323,293 - bet JDDGAC_27715 0.42 -2.2
5,324,033 + -0.2
5,324,033 + +1.8
5,324,036 + +0.6
5,324,102 + -2.4
5,324,183 + JDDGAC_27725 0.29 +0.2
5,324,183 + JDDGAC_27725 0.29 -1.6
5,324,184 - JDDGAC_27725 0.30 +0.6
5,324,229 + JDDGAC_27725 0.52 +1.0
5,324,363 + -0.6
5,324,365 + +1.0
5,324,416 + +0.5
5,324,417 - +0.1
5,324,661 + -0.3
5,324,662 - +0.9
5,324,683 + -0.2

Or see this region's nucleotide sequence