Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potA and potB overlap by 17 nucleotides potB and espK are separated by 363 nucleotides
JDDGAC_06740: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,173,055 to 1,174,191
potA
JDDGAC_06745: potB - spermidine/putrescine ABC transporter permease PotB, at 1,174,175 to 1,175,038
potB
JDDGAC_06750: espK - type III secretion system effector EspK, at 1,175,402 to 1,176,763
espK
Position (kb)
1174
1175
1176 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1173.483 kb on + strand, within potA at 1173.639 kb on + strand, within potA at 1173.882 kb on + strand, within potA at 1173.882 kb on + strand, within potA at 1173.885 kb on + strand, within potA at 1173.885 kb on + strand, within potA at 1173.889 kb on + strand, within potA at 1173.950 kb on + strand, within potA at 1173.951 kb on - strand, within potA at 1174.189 kb on + strand at 1174.189 kb on + strand at 1174.203 kb on + strand at 1174.331 kb on + strand, within potB at 1174.331 kb on + strand, within potB at 1174.355 kb on + strand, within potB at 1174.368 kb on + strand, within potB at 1174.369 kb on - strand, within potB at 1174.527 kb on - strand, within potB at 1174.559 kb on + strand, within potB at 1174.771 kb on + strand, within potB at 1174.841 kb on + strand, within potB at 1174.841 kb on + strand, within potB at 1174.899 kb on + strand, within potB at 1174.962 kb on + strand at 1175.286 kb on + strand at 1175.350 kb on + strand at 1175.359 kb on + strand at 1175.367 kb on - strand at 1175.529 kb on - strand at 1175.621 kb on - strand, within espK at 1175.635 kb on + strand, within espK at 1175.786 kb on + strand, within espK at 1175.786 kb on + strand, within espK at 1175.790 kb on + strand, within espK at 1175.895 kb on - strand, within espK at 1175.975 kb on - strand, within espK at 1175.977 kb on + strand, within espK at 1175.977 kb on + strand, within espK at 1175.978 kb on - strand, within espK
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 1,173,483 + potA JDDGAC_06740 0.38 +0.1 1,173,639 + potA JDDGAC_06740 0.51 +0.9 1,173,882 + potA JDDGAC_06740 0.73 -0.6 1,173,882 + potA JDDGAC_06740 0.73 +1.6 1,173,885 + potA JDDGAC_06740 0.73 -0.5 1,173,885 + potA JDDGAC_06740 0.73 +0.8 1,173,889 + potA JDDGAC_06740 0.73 +0.8 1,173,950 + potA JDDGAC_06740 0.79 +0.1 1,173,951 - potA JDDGAC_06740 0.79 +0.0 1,174,189 + -0.6 1,174,189 + +0.2 1,174,203 + +0.1 1,174,331 + potB JDDGAC_06745 0.18 -0.9 1,174,331 + potB JDDGAC_06745 0.18 +0.6 1,174,355 + potB JDDGAC_06745 0.21 -0.9 1,174,368 + potB JDDGAC_06745 0.22 -0.3 1,174,369 - potB JDDGAC_06745 0.22 -0.6 1,174,527 - potB JDDGAC_06745 0.41 -0.8 1,174,559 + potB JDDGAC_06745 0.44 -2.8 1,174,771 + potB JDDGAC_06745 0.69 -1.1 1,174,841 + potB JDDGAC_06745 0.77 +0.6 1,174,841 + potB JDDGAC_06745 0.77 -0.6 1,174,899 + potB JDDGAC_06745 0.84 -0.7 1,174,962 + -0.4 1,175,286 + -1.5 1,175,350 + -4.4 1,175,359 + +1.6 1,175,367 - +0.4 1,175,529 - -0.3 1,175,621 - espK JDDGAC_06750 0.16 -0.5 1,175,635 + espK JDDGAC_06750 0.17 +1.0 1,175,786 + espK JDDGAC_06750 0.28 -1.1 1,175,786 + espK JDDGAC_06750 0.28 +0.6 1,175,790 + espK JDDGAC_06750 0.28 -1.7 1,175,895 - espK JDDGAC_06750 0.36 -0.4 1,175,975 - espK JDDGAC_06750 0.42 +1.0 1,175,977 + espK JDDGAC_06750 0.42 +0.6 1,175,977 + espK JDDGAC_06750 0.42 +0.7 1,175,978 - espK JDDGAC_06750 0.42 +1.2
Or see this region's nucleotide sequence