Experiment: WM_Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uxaB and tam are separated by 277 nucleotides tam and lsrG are separated by 56 nucleotides lsrG and lsrF are separated by 23 nucleotides lsrF and lsrB are separated by 26 nucleotides
JDDGAC_03640: uxaB - tagaturonate reductase, at 605,932 to 607,383
uxaB
JDDGAC_03645: tam - trans-aconitate 2-methyltransferase, at 607,661 to 607,927
tam
JDDGAC_03650: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 607,984 to 608,274
lsrG
JDDGAC_03655: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 608,298 to 609,173
lsrF
JDDGAC_03660: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 609,200 to 610,222
lsrB
Position (kb) 607
608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 607.384 kb on + strand at 607.576 kb on - strand at 607.650 kb on + strand at 607.827 kb on + strand, within tam at 607.828 kb on - strand, within tam at 607.902 kb on + strand at 607.905 kb on - strand at 608.108 kb on + strand, within lsrG at 608.318 kb on - strand at 608.327 kb on - strand at 608.558 kb on - strand, within lsrF at 608.617 kb on + strand, within lsrF at 608.920 kb on - strand, within lsrF at 608.923 kb on - strand, within lsrF at 608.956 kb on - strand, within lsrF at 608.956 kb on - strand, within lsrF at 609.153 kb on - strand at 609.191 kb on + strand at 609.211 kb on - strand at 609.258 kb on - strand at 609.258 kb on - strand at 609.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas58 remove 607,384 + +1.2 607,576 - -1.2 607,650 + -0.1 607,827 + tam JDDGAC_03645 0.62 -1.0 607,828 - tam JDDGAC_03645 0.63 +0.4 607,902 + +0.6 607,905 - +0.7 608,108 + lsrG JDDGAC_03650 0.43 -1.2 608,318 - -2.4 608,327 - -1.9 608,558 - lsrF JDDGAC_03655 0.30 -0.0 608,617 + lsrF JDDGAC_03655 0.36 +0.5 608,920 - lsrF JDDGAC_03655 0.71 -1.6 608,923 - lsrF JDDGAC_03655 0.71 +1.1 608,956 - lsrF JDDGAC_03655 0.75 +0.4 608,956 - lsrF JDDGAC_03655 0.75 -1.1 609,153 - -0.2 609,191 + -0.1 609,211 - -0.7 609,258 - -3.0 609,258 - -0.0 609,258 - +0.5
Or see this region's nucleotide sequence