Experiment: defined media with D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05780 and LRK53_RS05785 are separated by 15 nucleotides LRK53_RS05785 and LRK53_RS05790 are separated by 9 nucleotides LRK53_RS05790 and LRK53_RS05795 overlap by 4 nucleotides LRK53_RS05795 and LRK53_RS05800 overlap by 4 nucleotides
LRK53_RS05780: LRK53_RS05780 - type II secretion system protein N, at 1,343,374 to 1,344,162
_RS05780
LRK53_RS05785: LRK53_RS05785 - inositol-phosphate phosphatase, at 1,344,178 to 1,344,966
_RS05785
LRK53_RS05790: LRK53_RS05790 - ADP compounds hydrolase NudE, at 1,344,976 to 1,345,533
_RS05790
LRK53_RS05795: LRK53_RS05795 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 1,345,530 to 1,346,351
_RS05795
LRK53_RS05800: LRK53_RS05800 - nucleoside triphosphate pyrophosphohydrolase, at 1,346,348 to 1,347,160
_RS05800
Position (kb) 1344
1345
1346 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.253 kb on + strand at 1344.256 kb on + strand at 1344.257 kb on - strand, within LRK53_RS05785 at 1344.257 kb on - strand, within LRK53_RS05785 at 1344.257 kb on - strand, within LRK53_RS05785 at 1344.257 kb on - strand, within LRK53_RS05785 at 1344.379 kb on + strand, within LRK53_RS05785 at 1344.379 kb on + strand, within LRK53_RS05785 at 1344.379 kb on + strand, within LRK53_RS05785 at 1344.379 kb on + strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.380 kb on - strand, within LRK53_RS05785 at 1344.527 kb on + strand, within LRK53_RS05785 at 1344.527 kb on + strand, within LRK53_RS05785 at 1344.544 kb on + strand, within LRK53_RS05785 at 1344.545 kb on - strand, within LRK53_RS05785 at 1344.748 kb on + strand, within LRK53_RS05785 at 1344.749 kb on - strand, within LRK53_RS05785 at 1344.749 kb on - strand, within LRK53_RS05785 at 1344.964 kb on + strand at 1344.964 kb on + strand at 1344.965 kb on - strand at 1344.987 kb on - strand at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.081 kb on + strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.082 kb on - strand, within LRK53_RS05790 at 1345.165 kb on + strand, within LRK53_RS05790 at 1345.186 kb on + strand, within LRK53_RS05790 at 1345.187 kb on - strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.324 kb on + strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.325 kb on - strand, within LRK53_RS05790 at 1345.504 kb on + strand at 1345.504 kb on + strand at 1345.505 kb on - strand at 1345.550 kb on + strand at 1345.550 kb on + strand at 1345.551 kb on - strand at 1345.551 kb on - strand at 1345.551 kb on - strand at 1345.620 kb on + strand, within LRK53_RS05795 at 1345.620 kb on + strand, within LRK53_RS05795 at 1345.620 kb on + strand, within LRK53_RS05795 at 1345.620 kb on + strand, within LRK53_RS05795 at 1345.621 kb on - strand, within LRK53_RS05795 at 1345.621 kb on - strand, within LRK53_RS05795 at 1345.621 kb on - strand, within LRK53_RS05795 at 1345.795 kb on - strand, within LRK53_RS05795 at 1345.821 kb on - strand, within LRK53_RS05795 at 1345.821 kb on - strand, within LRK53_RS05795 at 1345.821 kb on - strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.929 kb on + strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.930 kb on - strand, within LRK53_RS05795 at 1345.997 kb on + strand, within LRK53_RS05795 at 1345.997 kb on + strand, within LRK53_RS05795 at 1345.997 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.091 kb on + strand, within LRK53_RS05795 at 1346.092 kb on - strand, within LRK53_RS05795 at 1346.092 kb on - strand, within LRK53_RS05795 at 1346.092 kb on - strand, within LRK53_RS05795 at 1346.092 kb on - strand, within LRK53_RS05795 at 1346.101 kb on + strand, within LRK53_RS05795 at 1346.101 kb on + strand, within LRK53_RS05795 at 1346.101 kb on + strand, within LRK53_RS05795 at 1346.102 kb on - strand, within LRK53_RS05795 at 1346.157 kb on + strand, within LRK53_RS05795 at 1346.157 kb on + strand, within LRK53_RS05795 at 1346.157 kb on + strand, within LRK53_RS05795 at 1346.157 kb on + strand, within LRK53_RS05795 at 1346.157 kb on + strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.158 kb on - strand, within LRK53_RS05795 at 1346.262 kb on + strand, within LRK53_RS05795 at 1346.263 kb on - strand, within LRK53_RS05795 at 1346.331 kb on - strand at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.465 kb on + strand, within LRK53_RS05800 at 1346.466 kb on - strand, within LRK53_RS05800 at 1346.466 kb on - strand, within LRK53_RS05800 at 1346.466 kb on - strand, within LRK53_RS05800 at 1346.483 kb on + strand, within LRK53_RS05800 at 1346.483 kb on + strand, within LRK53_RS05800 at 1346.483 kb on + strand, within LRK53_RS05800 at 1346.483 kb on + strand, within LRK53_RS05800 at 1346.483 kb on + strand, within LRK53_RS05800 at 1346.484 kb on - strand, within LRK53_RS05800 at 1346.484 kb on - strand, within LRK53_RS05800 at 1346.484 kb on - strand, within LRK53_RS05800 at 1346.484 kb on - strand, within LRK53_RS05800
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with D-Glucose remove 1,344,253 + -2.7 1,344,253 + -1.1 1,344,253 + -2.3 1,344,253 + -0.7 1,344,253 + -2.0 1,344,253 + -1.1 1,344,253 + -2.1 1,344,253 + -2.7 1,344,253 + -3.5 1,344,256 + -4.2 1,344,257 - LRK53_RS05785 0.10 -0.1 1,344,257 - LRK53_RS05785 0.10 -2.1 1,344,257 - LRK53_RS05785 0.10 -1.4 1,344,257 - LRK53_RS05785 0.10 -1.7 1,344,379 + LRK53_RS05785 0.25 +0.9 1,344,379 + LRK53_RS05785 0.25 -0.7 1,344,379 + LRK53_RS05785 0.25 -1.1 1,344,379 + LRK53_RS05785 0.25 -1.1 1,344,380 - LRK53_RS05785 0.26 -2.5 1,344,380 - LRK53_RS05785 0.26 -1.9 1,344,380 - LRK53_RS05785 0.26 -1.3 1,344,380 - LRK53_RS05785 0.26 +0.9 1,344,380 - LRK53_RS05785 0.26 -2.6 1,344,380 - LRK53_RS05785 0.26 -1.9 1,344,380 - LRK53_RS05785 0.26 -2.0 1,344,527 + LRK53_RS05785 0.44 -0.7 1,344,527 + LRK53_RS05785 0.44 -0.7 1,344,544 + LRK53_RS05785 0.46 -0.7 1,344,545 - LRK53_RS05785 0.47 -0.7 1,344,748 + LRK53_RS05785 0.72 -1.1 1,344,749 - LRK53_RS05785 0.72 -1.1 1,344,749 - LRK53_RS05785 0.72 -1.7 1,344,964 + -0.7 1,344,964 + -1.1 1,344,965 - +0.5 1,344,987 - -1.1 1,345,081 + LRK53_RS05790 0.19 -1.4 1,345,081 + LRK53_RS05790 0.19 -1.3 1,345,081 + LRK53_RS05790 0.19 -0.7 1,345,081 + LRK53_RS05790 0.19 -0.7 1,345,081 + LRK53_RS05790 0.19 -0.7 1,345,081 + LRK53_RS05790 0.19 +0.6 1,345,081 + LRK53_RS05790 0.19 +0.9 1,345,081 + LRK53_RS05790 0.19 -1.1 1,345,081 + LRK53_RS05790 0.19 -0.4 1,345,081 + LRK53_RS05790 0.19 +0.9 1,345,081 + LRK53_RS05790 0.19 -0.1 1,345,081 + LRK53_RS05790 0.19 -0.7 1,345,081 + LRK53_RS05790 0.19 -0.1 1,345,082 - LRK53_RS05790 0.19 +0.9 1,345,082 - LRK53_RS05790 0.19 -0.1 1,345,082 - LRK53_RS05790 0.19 -1.7 1,345,082 - LRK53_RS05790 0.19 -1.1 1,345,082 - LRK53_RS05790 0.19 -0.9 1,345,082 - LRK53_RS05790 0.19 -3.0 1,345,082 - LRK53_RS05790 0.19 -1.5 1,345,082 - LRK53_RS05790 0.19 -1.9 1,345,165 + LRK53_RS05790 0.34 -0.7 1,345,186 + LRK53_RS05790 0.38 -0.4 1,345,187 - LRK53_RS05790 0.38 -1.3 1,345,324 + LRK53_RS05790 0.62 +0.9 1,345,324 + LRK53_RS05790 0.62 -0.1 1,345,324 + LRK53_RS05790 0.62 +0.9 1,345,324 + LRK53_RS05790 0.62 -0.4 1,345,324 + LRK53_RS05790 0.62 -0.1 1,345,324 + LRK53_RS05790 0.62 -0.2 1,345,324 + LRK53_RS05790 0.62 -1.1 1,345,324 + LRK53_RS05790 0.62 -0.1 1,345,325 - LRK53_RS05790 0.63 -1.1 1,345,325 - LRK53_RS05790 0.63 -1.4 1,345,325 - LRK53_RS05790 0.63 -0.1 1,345,325 - LRK53_RS05790 0.63 +0.3 1,345,325 - LRK53_RS05790 0.63 +0.9 1,345,325 - LRK53_RS05790 0.63 -1.9 1,345,325 - LRK53_RS05790 0.63 -0.2 1,345,325 - LRK53_RS05790 0.63 -1.1 1,345,325 - LRK53_RS05790 0.63 -1.1 1,345,325 - LRK53_RS05790 0.63 -1.1 1,345,325 - LRK53_RS05790 0.63 +0.3 1,345,325 - LRK53_RS05790 0.63 +0.3 1,345,504 + -1.7 1,345,504 + -1.1 1,345,505 - +0.5 1,345,550 + -0.7 1,345,550 + -0.1 1,345,551 - -2.0 1,345,551 - -0.7 1,345,551 - -0.3 1,345,620 + LRK53_RS05795 0.11 -0.1 1,345,620 + LRK53_RS05795 0.11 -0.7 1,345,620 + LRK53_RS05795 0.11 -1.9 1,345,620 + LRK53_RS05795 0.11 +1.9 1,345,621 - LRK53_RS05795 0.11 -0.4 1,345,621 - LRK53_RS05795 0.11 -2.3 1,345,621 - LRK53_RS05795 0.11 -4.4 1,345,795 - LRK53_RS05795 0.32 -2.5 1,345,821 - LRK53_RS05795 0.35 -1.1 1,345,821 - LRK53_RS05795 0.35 -1.1 1,345,821 - LRK53_RS05795 0.35 -2.1 1,345,929 + LRK53_RS05795 0.49 -2.3 1,345,929 + LRK53_RS05795 0.49 -2.5 1,345,929 + LRK53_RS05795 0.49 -1.4 1,345,929 + LRK53_RS05795 0.49 -0.1 1,345,929 + LRK53_RS05795 0.49 +2.5 1,345,929 + LRK53_RS05795 0.49 -1.1 1,345,929 + LRK53_RS05795 0.49 -3.0 1,345,929 + LRK53_RS05795 0.49 -0.7 1,345,929 + LRK53_RS05795 0.49 -1.4 1,345,929 + LRK53_RS05795 0.49 -2.3 1,345,929 + LRK53_RS05795 0.49 -1.6 1,345,929 + LRK53_RS05795 0.49 -2.3 1,345,929 + LRK53_RS05795 0.49 -1.9 1,345,929 + LRK53_RS05795 0.49 -1.0 1,345,930 - LRK53_RS05795 0.49 -0.8 1,345,930 - LRK53_RS05795 0.49 -0.9 1,345,930 - LRK53_RS05795 0.49 -3.1 1,345,930 - LRK53_RS05795 0.49 -1.1 1,345,930 - LRK53_RS05795 0.49 -0.1 1,345,930 - LRK53_RS05795 0.49 -2.9 1,345,930 - LRK53_RS05795 0.49 -1.9 1,345,930 - LRK53_RS05795 0.49 -1.4 1,345,930 - LRK53_RS05795 0.49 -1.6 1,345,930 - LRK53_RS05795 0.49 +0.9 1,345,930 - LRK53_RS05795 0.49 -3.7 1,345,930 - LRK53_RS05795 0.49 -1.9 1,345,930 - LRK53_RS05795 0.49 -2.7 1,345,930 - LRK53_RS05795 0.49 -1.4 1,345,997 + LRK53_RS05795 0.57 -2.6 1,345,997 + LRK53_RS05795 0.57 -1.3 1,345,997 + LRK53_RS05795 0.57 -3.3 1,346,091 + LRK53_RS05795 0.68 -2.4 1,346,091 + LRK53_RS05795 0.68 -2.6 1,346,091 + LRK53_RS05795 0.68 -1.9 1,346,091 + LRK53_RS05795 0.68 -1.7 1,346,091 + LRK53_RS05795 0.68 -2.6 1,346,091 + LRK53_RS05795 0.68 -1.6 1,346,092 - LRK53_RS05795 0.68 -1.8 1,346,092 - LRK53_RS05795 0.68 -2.8 1,346,092 - LRK53_RS05795 0.68 -0.1 1,346,092 - LRK53_RS05795 0.68 -2.1 1,346,101 + LRK53_RS05795 0.69 -0.1 1,346,101 + LRK53_RS05795 0.69 +0.2 1,346,101 + LRK53_RS05795 0.69 -0.4 1,346,102 - LRK53_RS05795 0.70 +0.5 1,346,157 + LRK53_RS05795 0.76 -2.1 1,346,157 + LRK53_RS05795 0.76 +0.9 1,346,157 + LRK53_RS05795 0.76 -1.6 1,346,157 + LRK53_RS05795 0.76 -1.1 1,346,157 + LRK53_RS05795 0.76 -2.2 1,346,158 - LRK53_RS05795 0.76 -1.4 1,346,158 - LRK53_RS05795 0.76 -1.7 1,346,158 - LRK53_RS05795 0.76 +0.3 1,346,158 - LRK53_RS05795 0.76 -0.7 1,346,158 - LRK53_RS05795 0.76 -0.7 1,346,158 - LRK53_RS05795 0.76 -2.7 1,346,158 - LRK53_RS05795 0.76 +0.5 1,346,262 + LRK53_RS05795 0.89 -0.7 1,346,263 - LRK53_RS05795 0.89 -1.3 1,346,331 - -1.4 1,346,465 + LRK53_RS05800 0.14 -0.7 1,346,465 + LRK53_RS05800 0.14 -0.3 1,346,465 + LRK53_RS05800 0.14 +0.9 1,346,465 + LRK53_RS05800 0.14 -2.6 1,346,465 + LRK53_RS05800 0.14 +1.9 1,346,465 + LRK53_RS05800 0.14 -1.9 1,346,465 + LRK53_RS05800 0.14 -1.1 1,346,465 + LRK53_RS05800 0.14 -1.1 1,346,465 + LRK53_RS05800 0.14 +0.3 1,346,465 + LRK53_RS05800 0.14 -0.2 1,346,466 - LRK53_RS05800 0.15 -0.8 1,346,466 - LRK53_RS05800 0.15 -1.4 1,346,466 - LRK53_RS05800 0.15 -0.7 1,346,483 + LRK53_RS05800 0.17 -2.1 1,346,483 + LRK53_RS05800 0.17 -1.7 1,346,483 + LRK53_RS05800 0.17 -0.7 1,346,483 + LRK53_RS05800 0.17 +0.9 1,346,483 + LRK53_RS05800 0.17 +0.9 1,346,484 - LRK53_RS05800 0.17 -0.1 1,346,484 - LRK53_RS05800 0.17 -0.1 1,346,484 - LRK53_RS05800 0.17 +0.9 1,346,484 - LRK53_RS05800 0.17 +1.9
Or see this region's nucleotide sequence