Strain Fitness in Escherichia coli ECRC98 around JDDGAC_24220

Experiment: WM_Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypD and hypC overlap by 1 nucleotideshypC and hypB overlap by 10 nucleotideshypB and hypA are separated by 3 nucleotides JDDGAC_24215: hypD - hydrogenase formation protein HypD, at 4,643,487 to 4,644,608 hypD JDDGAC_24220: hypC - hydrogenase 3 maturation protein HypC, at 4,644,608 to 4,644,880 hypC JDDGAC_24225: hypB - hydrogenase nickel incorporation protein HypB, at 4,644,871 to 4,645,743 hypB JDDGAC_24230: hypA - hydrogenase maturation nickel metallochaperone HypA, at 4,645,747 to 4,646,097 hypA Position (kb) 4644 4645Strain fitness (log2 ratio) -2 -1 0 1 2at 4643.615 kb on + strand, within hypDat 4643.859 kb on + strand, within hypDat 4643.914 kb on - strand, within hypDat 4643.978 kb on - strand, within hypDat 4643.999 kb on + strand, within hypDat 4644.000 kb on - strand, within hypDat 4644.000 kb on - strand, within hypDat 4644.003 kb on - strand, within hypDat 4644.003 kb on - strand, within hypDat 4644.080 kb on + strand, within hypDat 4644.080 kb on + strand, within hypDat 4644.081 kb on - strand, within hypDat 4644.083 kb on + strand, within hypDat 4644.083 kb on + strand, within hypDat 4644.083 kb on + strand, within hypDat 4644.084 kb on - strand, within hypDat 4644.101 kb on - strand, within hypDat 4644.523 kb on + strandat 4644.624 kb on + strandat 4644.624 kb on + strandat 4644.625 kb on - strandat 4644.704 kb on - strand, within hypCat 4644.704 kb on - strand, within hypCat 4644.707 kb on + strand, within hypCat 4644.707 kb on + strand, within hypCat 4645.062 kb on + strand, within hypBat 4645.063 kb on - strand, within hypBat 4645.153 kb on + strand, within hypBat 4645.154 kb on - strand, within hypBat 4645.614 kb on + strand, within hypBat 4645.615 kb on - strand, within hypBat 4645.665 kb on + strandat 4645.686 kb on + strandat 4645.706 kb on + strandat 4645.706 kb on + strandat 4645.706 kb on + strandat 4645.737 kb on + strandat 4645.738 kb on - strandat 4645.738 kb on - strandat 4645.759 kb on + strandat 4645.760 kb on - strandat 4645.765 kb on + strandat 4645.765 kb on + strandat 4645.804 kb on + strand, within hypAat 4645.804 kb on + strand, within hypAat 4645.863 kb on - strand, within hypAat 4645.863 kb on - strand, within hypA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas61
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4,643,615 + hypD JDDGAC_24215 0.11 -2.5
4,643,859 + hypD JDDGAC_24215 0.33 -1.5
4,643,914 - hypD JDDGAC_24215 0.38 +1.3
4,643,978 - hypD JDDGAC_24215 0.44 +0.4
4,643,999 + hypD JDDGAC_24215 0.46 -1.9
4,644,000 - hypD JDDGAC_24215 0.46 +0.4
4,644,000 - hypD JDDGAC_24215 0.46 -0.6
4,644,003 - hypD JDDGAC_24215 0.46 -0.1
4,644,003 - hypD JDDGAC_24215 0.46 -1.4
4,644,080 + hypD JDDGAC_24215 0.53 -1.1
4,644,080 + hypD JDDGAC_24215 0.53 +0.4
4,644,081 - hypD JDDGAC_24215 0.53 -0.7
4,644,083 + hypD JDDGAC_24215 0.53 -0.4
4,644,083 + hypD JDDGAC_24215 0.53 +0.6
4,644,083 + hypD JDDGAC_24215 0.53 +0.7
4,644,084 - hypD JDDGAC_24215 0.53 -2.7
4,644,101 - hypD JDDGAC_24215 0.55 +0.6
4,644,523 + +0.8
4,644,624 + -0.3
4,644,624 + +2.2
4,644,625 - +0.5
4,644,704 - hypC JDDGAC_24220 0.35 -1.4
4,644,704 - hypC JDDGAC_24220 0.35 -0.8
4,644,707 + hypC JDDGAC_24220 0.36 -2.0
4,644,707 + hypC JDDGAC_24220 0.36 -0.6
4,645,062 + hypB JDDGAC_24225 0.22 -0.4
4,645,063 - hypB JDDGAC_24225 0.22 +1.0
4,645,153 + hypB JDDGAC_24225 0.32 +0.9
4,645,154 - hypB JDDGAC_24225 0.32 -1.0
4,645,614 + hypB JDDGAC_24225 0.85 -0.3
4,645,615 - hypB JDDGAC_24225 0.85 +0.8
4,645,665 + +0.0
4,645,686 + +0.8
4,645,706 + +1.1
4,645,706 + -1.8
4,645,706 + -0.5
4,645,737 + +1.0
4,645,738 - +0.9
4,645,738 - +0.5
4,645,759 + +0.6
4,645,760 - -0.3
4,645,765 + +1.1
4,645,765 + +0.2
4,645,804 + hypA JDDGAC_24230 0.16 -0.5
4,645,804 + hypA JDDGAC_24230 0.16 +0.3
4,645,863 - hypA JDDGAC_24230 0.33 -0.2
4,645,863 - hypA JDDGAC_24230 0.33 +0.1

Or see this region's nucleotide sequence