Experiment: defined media with D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06835 and LRK53_RS06840 are separated by 71 nucleotides LRK53_RS06840 and LRK53_RS06845 are separated by 159 nucleotides LRK53_RS06845 and LRK53_RS06850 are separated by 158 nucleotides
LRK53_RS06835: LRK53_RS06835 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 1,543,409 to 1,544,455
_RS06835
LRK53_RS06840: LRK53_RS06840 - tRNA guanosine(34) transglycosylase Tgt, at 1,544,527 to 1,545,654
_RS06840
LRK53_RS06845: LRK53_RS06845 - preprotein translocase subunit YajC, at 1,545,814 to 1,546,146
_RS06845
LRK53_RS06850: LRK53_RS06850 - protein translocase subunit SecD, at 1,546,305 to 1,548,188
_RS06850
Position (kb)
1544
1545
1546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1543.613 kb on + strand, within LRK53_RS06835 at 1543.613 kb on + strand, within LRK53_RS06835 at 1543.614 kb on - strand, within LRK53_RS06835 at 1543.614 kb on - strand, within LRK53_RS06835 at 1543.856 kb on + strand, within LRK53_RS06835 at 1543.856 kb on + strand, within LRK53_RS06835 at 1543.856 kb on + strand, within LRK53_RS06835 at 1543.856 kb on + strand, within LRK53_RS06835 at 1543.856 kb on + strand, within LRK53_RS06835 at 1543.857 kb on - strand, within LRK53_RS06835 at 1543.857 kb on - strand, within LRK53_RS06835 at 1543.896 kb on - strand, within LRK53_RS06835 at 1543.896 kb on - strand, within LRK53_RS06835 at 1544.006 kb on + strand, within LRK53_RS06835 at 1544.006 kb on + strand, within LRK53_RS06835 at 1544.007 kb on - strand, within LRK53_RS06835 at 1544.007 kb on - strand, within LRK53_RS06835 at 1544.007 kb on - strand, within LRK53_RS06835 at 1544.402 kb on + strand at 1544.403 kb on - strand at 1544.403 kb on - strand at 1544.446 kb on + strand at 1544.518 kb on - strand at 1544.639 kb on - strand at 1544.639 kb on - strand at 1544.706 kb on + strand, within LRK53_RS06840 at 1544.706 kb on + strand, within LRK53_RS06840 at 1544.707 kb on - strand, within LRK53_RS06840 at 1544.707 kb on - strand, within LRK53_RS06840 at 1545.106 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.406 kb on + strand, within LRK53_RS06840 at 1545.407 kb on - strand, within LRK53_RS06840 at 1545.407 kb on - strand, within LRK53_RS06840 at 1545.407 kb on - strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.448 kb on + strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.449 kb on - strand, within LRK53_RS06840 at 1545.478 kb on + strand, within LRK53_RS06840 at 1545.478 kb on + strand, within LRK53_RS06840 at 1545.479 kb on - strand, within LRK53_RS06840 at 1545.547 kb on + strand at 1545.550 kb on + strand at 1545.551 kb on - strand at 1545.622 kb on + strand at 1545.622 kb on + strand at 1545.652 kb on + strand at 1545.653 kb on - strand at 1545.745 kb on + strand at 1545.760 kb on + strand at 1545.795 kb on + strand at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.878 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1545.919 kb on + strand, within LRK53_RS06845 at 1546.181 kb on + strand at 1546.181 kb on + strand at 1546.181 kb on + strand at 1546.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with D-Glucose remove 1,543,613 + LRK53_RS06835 0.19 +0.4 1,543,613 + LRK53_RS06835 0.19 -1.7 1,543,614 - LRK53_RS06835 0.20 -0.9 1,543,614 - LRK53_RS06835 0.20 +1.4 1,543,856 + LRK53_RS06835 0.43 -1.8 1,543,856 + LRK53_RS06835 0.43 -0.3 1,543,856 + LRK53_RS06835 0.43 -0.1 1,543,856 + LRK53_RS06835 0.43 -0.3 1,543,856 + LRK53_RS06835 0.43 +0.9 1,543,857 - LRK53_RS06835 0.43 -1.7 1,543,857 - LRK53_RS06835 0.43 +0.9 1,543,896 - LRK53_RS06835 0.47 -1.1 1,543,896 - LRK53_RS06835 0.47 -0.7 1,544,006 + LRK53_RS06835 0.57 +0.9 1,544,006 + LRK53_RS06835 0.57 -0.1 1,544,007 - LRK53_RS06835 0.57 +1.9 1,544,007 - LRK53_RS06835 0.57 -1.7 1,544,007 - LRK53_RS06835 0.57 +0.1 1,544,402 + -0.6 1,544,403 - -1.3 1,544,403 - +0.9 1,544,446 + -0.7 1,544,518 - -0.7 1,544,639 - -0.7 1,544,639 - -0.6 1,544,706 + LRK53_RS06840 0.16 -2.1 1,544,706 + LRK53_RS06840 0.16 +0.9 1,544,707 - LRK53_RS06840 0.16 +1.9 1,544,707 - LRK53_RS06840 0.16 +0.5 1,545,106 + LRK53_RS06840 0.51 +0.6 1,545,406 + LRK53_RS06840 0.78 -0.7 1,545,406 + LRK53_RS06840 0.78 -1.3 1,545,406 + LRK53_RS06840 0.78 -1.1 1,545,406 + LRK53_RS06840 0.78 -0.1 1,545,407 - LRK53_RS06840 0.78 +0.4 1,545,407 - LRK53_RS06840 0.78 -1.5 1,545,407 - LRK53_RS06840 0.78 -3.2 1,545,448 + LRK53_RS06840 0.82 -0.7 1,545,448 + LRK53_RS06840 0.82 +0.9 1,545,448 + LRK53_RS06840 0.82 -2.0 1,545,448 + LRK53_RS06840 0.82 +0.9 1,545,449 - LRK53_RS06840 0.82 -1.1 1,545,449 - LRK53_RS06840 0.82 -0.1 1,545,478 + LRK53_RS06840 0.84 -1.6 1,545,478 + LRK53_RS06840 0.84 -0.1 1,545,479 - LRK53_RS06840 0.84 -0.7 1,545,547 + +0.9 1,545,550 + +0.6 1,545,551 - -0.7 1,545,622 + +0.3 1,545,622 + +0.9 1,545,652 + -2.0 1,545,653 - +0.9 1,545,745 + -0.7 1,545,760 + -0.7 1,545,795 + -1.7 1,545,878 + LRK53_RS06845 0.19 +0.0 1,545,878 + LRK53_RS06845 0.19 +0.2 1,545,878 + LRK53_RS06845 0.19 -0.8 1,545,878 + LRK53_RS06845 0.19 -0.5 1,545,878 + LRK53_RS06845 0.19 -2.0 1,545,878 + LRK53_RS06845 0.19 +0.9 1,545,878 + LRK53_RS06845 0.19 -0.7 1,545,919 + LRK53_RS06845 0.32 +0.3 1,545,919 + LRK53_RS06845 0.32 -0.5 1,545,919 + LRK53_RS06845 0.32 -0.1 1,545,919 + LRK53_RS06845 0.32 -0.6 1,545,919 + LRK53_RS06845 0.32 +0.9 1,545,919 + LRK53_RS06845 0.32 -0.7 1,545,919 + LRK53_RS06845 0.32 -0.1 1,545,919 + LRK53_RS06845 0.32 -0.9 1,545,919 + LRK53_RS06845 0.32 +1.2 1,545,919 + LRK53_RS06845 0.32 +0.3 1,545,919 + LRK53_RS06845 0.32 -1.7 1,545,919 + LRK53_RS06845 0.32 -1.1 1,545,919 + LRK53_RS06845 0.32 -0.1 1,545,919 + LRK53_RS06845 0.32 -0.1 1,545,919 + LRK53_RS06845 0.32 -0.7 1,546,181 + -0.5 1,546,181 + -0.1 1,546,181 + -1.0 1,546,232 + -0.9
Or see this region's nucleotide sequence