Experiment: WM_Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt garD and garP are separated by 374 nucleotides garP and garL are separated by 15 nucleotides garL and garR are separated by 29 nucleotides
JDDGAC_21985: garD - galactarate dehydratase, at 4,201,996 to 4,203,567
garD
JDDGAC_21990: garP - galactarate/glucarate/glycerate transporter GarP, at 4,203,942 to 4,205,276
garP
JDDGAC_21995: garL - 2-dehydro-3-deoxyglucarate aldolase, at 4,205,292 to 4,206,062
garL
JDDGAC_22000: garR - 2-hydroxy-3-oxopropionate reductase, at 4,206,092 to 4,206,982
garR
Position (kb)
4203
4204
4205
4206 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4202.944 kb on + strand, within garD at 4202.944 kb on + strand, within garD at 4202.945 kb on - strand, within garD at 4202.962 kb on - strand, within garD at 4202.998 kb on + strand, within garD at 4202.998 kb on + strand, within garD at 4202.998 kb on + strand, within garD at 4202.998 kb on + strand, within garD at 4202.999 kb on - strand, within garD at 4202.999 kb on - strand, within garD at 4202.999 kb on - strand, within garD at 4203.112 kb on + strand, within garD at 4203.112 kb on + strand, within garD at 4203.113 kb on - strand, within garD at 4203.113 kb on - strand, within garD at 4203.116 kb on + strand, within garD at 4203.117 kb on - strand, within garD at 4203.292 kb on + strand, within garD at 4203.293 kb on - strand, within garD at 4203.369 kb on + strand, within garD at 4203.433 kb on + strand at 4203.889 kb on + strand at 4203.994 kb on + strand at 4204.064 kb on + strand at 4204.090 kb on + strand, within garP at 4204.203 kb on + strand, within garP at 4204.204 kb on - strand, within garP at 4204.253 kb on + strand, within garP at 4204.254 kb on - strand, within garP at 4204.346 kb on + strand, within garP at 4204.413 kb on + strand, within garP at 4204.562 kb on + strand, within garP at 4204.562 kb on + strand, within garP at 4204.562 kb on + strand, within garP at 4204.562 kb on + strand, within garP at 4204.563 kb on - strand, within garP at 4204.563 kb on - strand, within garP at 4204.563 kb on - strand, within garP at 4204.563 kb on - strand, within garP at 4204.587 kb on - strand, within garP at 4204.665 kb on + strand, within garP at 4204.666 kb on - strand, within garP at 4204.787 kb on + strand, within garP at 4204.913 kb on + strand, within garP at 4204.914 kb on - strand, within garP at 4204.914 kb on - strand, within garP at 4204.960 kb on + strand, within garP at 4204.964 kb on + strand, within garP at 4204.965 kb on - strand, within garP at 4204.968 kb on + strand, within garP at 4204.968 kb on + strand, within garP at 4204.968 kb on + strand, within garP at 4204.969 kb on - strand, within garP at 4204.969 kb on - strand, within garP at 4205.158 kb on + strand at 4205.253 kb on - strand at 4205.253 kb on - strand at 4205.259 kb on - strand at 4205.274 kb on + strand at 4205.284 kb on + strand at 4205.284 kb on + strand at 4205.285 kb on - strand at 4205.385 kb on - strand, within garL at 4205.454 kb on - strand, within garL at 4205.510 kb on - strand, within garL at 4205.537 kb on - strand, within garL at 4205.540 kb on + strand, within garL at 4205.544 kb on - strand, within garL at 4205.682 kb on - strand, within garL at 4205.747 kb on - strand, within garL at 4205.769 kb on - strand, within garL at 4205.781 kb on - strand, within garL at 4205.781 kb on - strand, within garL at 4205.781 kb on - strand, within garL at 4205.843 kb on - strand, within garL at 4205.876 kb on - strand, within garL at 4205.876 kb on - strand, within garL at 4206.091 kb on + strand at 4206.091 kb on + strand at 4206.091 kb on + strand at 4206.092 kb on - strand at 4206.112 kb on + strand at 4206.145 kb on + strand at 4206.203 kb on - strand, within garR at 4206.203 kb on - strand, within garR
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas61 remove 4,202,944 + garD JDDGAC_21985 0.60 +1.5 4,202,944 + garD JDDGAC_21985 0.60 -0.5 4,202,945 - garD JDDGAC_21985 0.60 +0.5 4,202,962 - garD JDDGAC_21985 0.61 -2.2 4,202,998 + garD JDDGAC_21985 0.64 -1.8 4,202,998 + garD JDDGAC_21985 0.64 +0.7 4,202,998 + garD JDDGAC_21985 0.64 +0.1 4,202,998 + garD JDDGAC_21985 0.64 +0.4 4,202,999 - garD JDDGAC_21985 0.64 -0.5 4,202,999 - garD JDDGAC_21985 0.64 +0.5 4,202,999 - garD JDDGAC_21985 0.64 +0.6 4,203,112 + garD JDDGAC_21985 0.71 -0.7 4,203,112 + garD JDDGAC_21985 0.71 +0.6 4,203,113 - garD JDDGAC_21985 0.71 -0.8 4,203,113 - garD JDDGAC_21985 0.71 -0.1 4,203,116 + garD JDDGAC_21985 0.71 +0.1 4,203,117 - garD JDDGAC_21985 0.71 -0.5 4,203,292 + garD JDDGAC_21985 0.82 +0.0 4,203,293 - garD JDDGAC_21985 0.83 +0.3 4,203,369 + garD JDDGAC_21985 0.87 -0.8 4,203,433 + -1.2 4,203,889 + +0.9 4,203,994 + +0.9 4,204,064 + -0.4 4,204,090 + garP JDDGAC_21990 0.11 +0.5 4,204,203 + garP JDDGAC_21990 0.20 -0.5 4,204,204 - garP JDDGAC_21990 0.20 -0.0 4,204,253 + garP JDDGAC_21990 0.23 +0.0 4,204,254 - garP JDDGAC_21990 0.23 -0.6 4,204,346 + garP JDDGAC_21990 0.30 -1.1 4,204,413 + garP JDDGAC_21990 0.35 -1.7 4,204,562 + garP JDDGAC_21990 0.46 +0.2 4,204,562 + garP JDDGAC_21990 0.46 +0.3 4,204,562 + garP JDDGAC_21990 0.46 -0.7 4,204,562 + garP JDDGAC_21990 0.46 -0.5 4,204,563 - garP JDDGAC_21990 0.47 -1.4 4,204,563 - garP JDDGAC_21990 0.47 -1.8 4,204,563 - garP JDDGAC_21990 0.47 -1.6 4,204,563 - garP JDDGAC_21990 0.47 -0.8 4,204,587 - garP JDDGAC_21990 0.48 -2.3 4,204,665 + garP JDDGAC_21990 0.54 -0.1 4,204,666 - garP JDDGAC_21990 0.54 -0.2 4,204,787 + garP JDDGAC_21990 0.63 -0.7 4,204,913 + garP JDDGAC_21990 0.73 -0.3 4,204,914 - garP JDDGAC_21990 0.73 -2.1 4,204,914 - garP JDDGAC_21990 0.73 -0.4 4,204,960 + garP JDDGAC_21990 0.76 -0.0 4,204,964 + garP JDDGAC_21990 0.77 -1.5 4,204,965 - garP JDDGAC_21990 0.77 -0.1 4,204,968 + garP JDDGAC_21990 0.77 +1.5 4,204,968 + garP JDDGAC_21990 0.77 -0.4 4,204,968 + garP JDDGAC_21990 0.77 +0.7 4,204,969 - garP JDDGAC_21990 0.77 -1.0 4,204,969 - garP JDDGAC_21990 0.77 -0.2 4,205,158 + -1.8 4,205,253 - -0.0 4,205,253 - -0.0 4,205,259 - -0.1 4,205,274 + -0.2 4,205,284 + +0.7 4,205,284 + -0.4 4,205,285 - -0.2 4,205,385 - garL JDDGAC_21995 0.12 +2.2 4,205,454 - garL JDDGAC_21995 0.21 +0.5 4,205,510 - garL JDDGAC_21995 0.28 -1.2 4,205,537 - garL JDDGAC_21995 0.32 +0.8 4,205,540 + garL JDDGAC_21995 0.32 -0.1 4,205,544 - garL JDDGAC_21995 0.33 -1.4 4,205,682 - garL JDDGAC_21995 0.51 +2.0 4,205,747 - garL JDDGAC_21995 0.59 +0.2 4,205,769 - garL JDDGAC_21995 0.62 -1.4 4,205,781 - garL JDDGAC_21995 0.63 +0.3 4,205,781 - garL JDDGAC_21995 0.63 -0.4 4,205,781 - garL JDDGAC_21995 0.63 +0.8 4,205,843 - garL JDDGAC_21995 0.71 +0.4 4,205,876 - garL JDDGAC_21995 0.76 +0.1 4,205,876 - garL JDDGAC_21995 0.76 -1.3 4,206,091 + -1.7 4,206,091 + +0.1 4,206,091 + +0.0 4,206,092 - +0.1 4,206,112 + -1.6 4,206,145 + +0.4 4,206,203 - garR JDDGAC_22000 0.12 -0.9 4,206,203 - garR JDDGAC_22000 0.12 -0.0
Or see this region's nucleotide sequence