Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS19105 and LRK53_RS19110 are separated by 61 nucleotides LRK53_RS19110 and LRK53_RS19115 are separated by 349 nucleotides LRK53_RS19115 and LRK53_RS19120 are separated by 11 nucleotides LRK53_RS19120 and LRK53_RS19125 are separated by 109 nucleotides LRK53_RS19125 and LRK53_RS19130 are separated by 78 nucleotides
LRK53_RS19105: LRK53_RS19105 - hypothetical protein, at 207,001 to 207,444
_RS19105
LRK53_RS19110: LRK53_RS19110 - hypothetical protein, at 207,506 to 207,709
_RS19110
LRK53_RS19115: LRK53_RS19115 - hypothetical protein, at 208,059 to 208,373
_RS19115
LRK53_RS19120: LRK53_RS19120 - hypothetical protein, at 208,385 to 208,666
_RS19120
LRK53_RS19125: LRK53_RS19125 - GPI inositol-deacylase, at 208,776 to 209,438
_RS19125
LRK53_RS19130: LRK53_RS19130 - DNA polymerase III subunit beta, at 209,517 to 210,620
_RS19130
Position (kb) 208
209 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 207.473 kb on + strand at 207.474 kb on - strand at 207.659 kb on + strand, within LRK53_RS19110 at 207.659 kb on + strand, within LRK53_RS19110 at 207.659 kb on + strand, within LRK53_RS19110 at 207.660 kb on - strand, within LRK53_RS19110 at 207.796 kb on + strand at 207.796 kb on + strand at 207.797 kb on - strand at 207.856 kb on + strand at 207.856 kb on + strand at 207.856 kb on + strand at 207.856 kb on + strand at 207.856 kb on + strand at 207.857 kb on - strand at 207.857 kb on - strand at 207.857 kb on - strand at 207.857 kb on - strand at 207.857 kb on - strand at 207.857 kb on - strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.881 kb on + strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 207.882 kb on - strand at 208.103 kb on + strand, within LRK53_RS19115 at 208.374 kb on + strand at 208.374 kb on + strand at 208.374 kb on + strand at 208.374 kb on + strand at 208.374 kb on + strand at 208.375 kb on - strand at 208.375 kb on - strand at 208.375 kb on - strand at 208.430 kb on + strand, within LRK53_RS19120 at 208.430 kb on + strand, within LRK53_RS19120 at 208.430 kb on + strand, within LRK53_RS19120 at 208.431 kb on - strand, within LRK53_RS19120 at 208.431 kb on - strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.499 kb on + strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.500 kb on - strand, within LRK53_RS19120 at 208.723 kb on + strand at 208.723 kb on + strand at 208.723 kb on + strand at 208.723 kb on + strand at 208.724 kb on - strand at 208.724 kb on - strand at 208.724 kb on - strand at 208.730 kb on - strand at 208.730 kb on - strand at 208.743 kb on - strand at 208.782 kb on + strand at 208.782 kb on + strand at 208.782 kb on + strand at 208.782 kb on + strand at 208.816 kb on + strand at 208.816 kb on + strand at 208.816 kb on + strand at 208.817 kb on - strand at 208.817 kb on - strand at 208.832 kb on + strand at 208.832 kb on + strand at 208.832 kb on + strand at 208.832 kb on + strand at 208.832 kb on + strand at 208.832 kb on + strand at 208.833 kb on - strand at 208.833 kb on - strand at 208.833 kb on - strand at 208.833 kb on - strand at 208.903 kb on - strand, within LRK53_RS19125 at 208.995 kb on + strand, within LRK53_RS19125 at 208.995 kb on + strand, within LRK53_RS19125 at 208.996 kb on - strand, within LRK53_RS19125 at 208.996 kb on - strand, within LRK53_RS19125 at 209.050 kb on + strand, within LRK53_RS19125 at 209.175 kb on + strand, within LRK53_RS19125 at 209.382 kb on + strand at 209.382 kb on + strand at 209.382 kb on + strand at 209.383 kb on - strand at 209.383 kb on - strand at 209.484 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 207,473 + -0.5 207,474 - -1.4 207,659 + LRK53_RS19110 0.75 +0.1 207,659 + LRK53_RS19110 0.75 -0.3 207,659 + LRK53_RS19110 0.75 -2.4 207,660 - LRK53_RS19110 0.75 -0.6 207,796 + -0.2 207,796 + -0.6 207,797 - +0.8 207,856 + -0.9 207,856 + +2.4 207,856 + -0.6 207,856 + -0.3 207,856 + -1.6 207,857 - -0.6 207,857 - -1.7 207,857 - -1.4 207,857 - +0.4 207,857 - +0.4 207,857 - -1.2 207,881 + -0.2 207,881 + -0.3 207,881 + -1.9 207,881 + -0.2 207,881 + -0.2 207,881 + +0.4 207,881 + +0.1 207,881 + +1.4 207,881 + -1.3 207,881 + -0.7 207,882 - -1.7 207,882 - +2.0 207,882 - -1.4 207,882 - -0.9 207,882 - -0.6 207,882 - -2.8 207,882 - +0.4 207,882 - -0.6 207,882 - -0.6 207,882 - -2.3 207,882 - -0.3 207,882 - -1.4 207,882 - -0.6 208,103 + LRK53_RS19115 0.14 -0.4 208,374 + -1.4 208,374 + -0.0 208,374 + -0.2 208,374 + -1.2 208,374 + -1.1 208,375 - +0.4 208,375 - -0.2 208,375 - +0.7 208,430 + LRK53_RS19120 0.16 -0.9 208,430 + LRK53_RS19120 0.16 -0.0 208,430 + LRK53_RS19120 0.16 -1.2 208,431 - LRK53_RS19120 0.16 +0.4 208,431 - LRK53_RS19120 0.16 -1.4 208,499 + LRK53_RS19120 0.40 -0.2 208,499 + LRK53_RS19120 0.40 +0.8 208,499 + LRK53_RS19120 0.40 -0.2 208,499 + LRK53_RS19120 0.40 -1.6 208,499 + LRK53_RS19120 0.40 -0.9 208,499 + LRK53_RS19120 0.40 -1.9 208,499 + LRK53_RS19120 0.40 -0.4 208,499 + LRK53_RS19120 0.40 -1.9 208,499 + LRK53_RS19120 0.40 -0.8 208,499 + LRK53_RS19120 0.40 +0.4 208,500 - LRK53_RS19120 0.41 -2.3 208,500 - LRK53_RS19120 0.41 +0.6 208,500 - LRK53_RS19120 0.41 -2.7 208,500 - LRK53_RS19120 0.41 -2.0 208,500 - LRK53_RS19120 0.41 -1.6 208,500 - LRK53_RS19120 0.41 +0.4 208,500 - LRK53_RS19120 0.41 -2.0 208,500 - LRK53_RS19120 0.41 -0.5 208,500 - LRK53_RS19120 0.41 -0.2 208,500 - LRK53_RS19120 0.41 -1.6 208,500 - LRK53_RS19120 0.41 +0.4 208,500 - LRK53_RS19120 0.41 -0.2 208,500 - LRK53_RS19120 0.41 -0.7 208,723 + -2.2 208,723 + -1.6 208,723 + +0.4 208,723 + +1.4 208,724 - -0.8 208,724 - +1.4 208,724 - -0.9 208,730 - -1.7 208,730 - -0.2 208,743 - -0.2 208,782 + +0.1 208,782 + +0.6 208,782 + +0.4 208,782 + -0.9 208,816 + -0.9 208,816 + +0.8 208,816 + -1.8 208,817 - -0.6 208,817 - -1.7 208,832 + -0.6 208,832 + -0.8 208,832 + -1.0 208,832 + +0.1 208,832 + -0.6 208,832 + +0.6 208,833 - -0.2 208,833 - -1.6 208,833 - -0.5 208,833 - +1.4 208,903 - LRK53_RS19125 0.19 -0.6 208,995 + LRK53_RS19125 0.33 -0.5 208,995 + LRK53_RS19125 0.33 -1.8 208,996 - LRK53_RS19125 0.33 -0.5 208,996 - LRK53_RS19125 0.33 +0.3 209,050 + LRK53_RS19125 0.41 +0.2 209,175 + LRK53_RS19125 0.60 -1.9 209,382 + -0.3 209,382 + +0.4 209,382 + +0.4 209,383 - -0.2 209,383 - -0.7 209,484 - -0.9
Or see this region's nucleotide sequence