Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03100 and LRK53_RS03105 overlap by 4 nucleotides LRK53_RS03105 and LRK53_RS03110 are separated by 157 nucleotides LRK53_RS03110 and LRK53_RS03115 overlap by 4 nucleotides LRK53_RS03115 and LRK53_RS03120 overlap by 4 nucleotides
LRK53_RS03100: LRK53_RS03100 - 3-isopropylmalate dehydrogenase, at 724,267 to 725,364
_RS03100
LRK53_RS03105: LRK53_RS03105 - 3-isopropylmalate dehydratase small subunit, at 725,361 to 726,005
_RS03105
LRK53_RS03110: LRK53_RS03110 - 3-isopropylmalate dehydratase large subunit, at 726,163 to 727,575
_RS03110
LRK53_RS03115: LRK53_RS03115 - branched-chain amino acid transaminase, at 727,572 to 728,573
_RS03115
LRK53_RS03120: LRK53_RS03120 - 2-isopropylmalate synthase, at 728,570 to 730,204
_RS03120
Position (kb) 726
727
728 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 725.353 kb on - strand at 725.370 kb on + strand at 725.482 kb on + strand, within LRK53_RS03105 at 725.483 kb on - strand, within LRK53_RS03105 at 725.483 kb on - strand, within LRK53_RS03105 at 725.483 kb on - strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.572 kb on + strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.573 kb on - strand, within LRK53_RS03105 at 725.728 kb on + strand, within LRK53_RS03105 at 725.729 kb on - strand, within LRK53_RS03105 at 725.729 kb on - strand, within LRK53_RS03105 at 725.729 kb on - strand, within LRK53_RS03105 at 726.155 kb on - strand at 726.185 kb on + strand at 726.186 kb on - strand at 726.186 kb on - strand at 726.186 kb on - strand at 726.186 kb on - strand at 726.186 kb on - strand at 726.605 kb on + strand, within LRK53_RS03110 at 726.605 kb on + strand, within LRK53_RS03110 at 726.605 kb on + strand, within LRK53_RS03110 at 726.605 kb on + strand, within LRK53_RS03110 at 726.605 kb on + strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.606 kb on - strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.689 kb on + strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.690 kb on - strand, within LRK53_RS03110 at 726.740 kb on + strand, within LRK53_RS03110 at 726.741 kb on - strand, within LRK53_RS03110 at 726.950 kb on + strand, within LRK53_RS03110 at 726.951 kb on - strand, within LRK53_RS03110 at 726.951 kb on - strand, within LRK53_RS03110 at 727.337 kb on + strand, within LRK53_RS03110 at 727.337 kb on + strand, within LRK53_RS03110 at 727.338 kb on - strand, within LRK53_RS03110 at 727.493 kb on + strand at 727.493 kb on + strand at 727.493 kb on + strand at 727.494 kb on - strand at 727.494 kb on - strand at 727.494 kb on - strand at 727.494 kb on - strand at 727.684 kb on + strand, within LRK53_RS03115 at 727.684 kb on + strand, within LRK53_RS03115 at 727.697 kb on + strand, within LRK53_RS03115 at 727.843 kb on + strand, within LRK53_RS03115 at 727.843 kb on + strand, within LRK53_RS03115 at 727.843 kb on + strand, within LRK53_RS03115 at 727.844 kb on - strand, within LRK53_RS03115 at 727.844 kb on - strand, within LRK53_RS03115 at 727.844 kb on - strand, within LRK53_RS03115 at 727.844 kb on - strand, within LRK53_RS03115 at 728.047 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.092 kb on + strand, within LRK53_RS03115 at 728.093 kb on - strand, within LRK53_RS03115 at 728.093 kb on - strand, within LRK53_RS03115 at 728.093 kb on - strand, within LRK53_RS03115 at 728.093 kb on - strand, within LRK53_RS03115 at 728.093 kb on - strand, within LRK53_RS03115 at 728.194 kb on + strand, within LRK53_RS03115 at 728.194 kb on + strand, within LRK53_RS03115 at 728.194 kb on + strand, within LRK53_RS03115 at 728.194 kb on + strand, within LRK53_RS03115 at 728.195 kb on - strand, within LRK53_RS03115 at 728.195 kb on - strand, within LRK53_RS03115 at 728.195 kb on - strand, within LRK53_RS03115 at 728.195 kb on - strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.296 kb on + strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.297 kb on - strand, within LRK53_RS03115 at 728.362 kb on + strand, within LRK53_RS03115 at 728.362 kb on + strand, within LRK53_RS03115 at 728.363 kb on - strand, within LRK53_RS03115 at 728.363 kb on - strand, within LRK53_RS03115 at 728.363 kb on - strand, within LRK53_RS03115 at 728.380 kb on + strand, within LRK53_RS03115 at 728.380 kb on + strand, within LRK53_RS03115 at 728.380 kb on + strand, within LRK53_RS03115 at 728.381 kb on - strand, within LRK53_RS03115 at 728.381 kb on - strand, within LRK53_RS03115 at 728.432 kb on - strand, within LRK53_RS03115 at 728.455 kb on + strand, within LRK53_RS03115 at 728.456 kb on - strand, within LRK53_RS03115 at 728.488 kb on + strand at 728.489 kb on - strand at 728.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 725,353 - -4.0 725,370 + -1.0 725,482 + LRK53_RS03105 0.19 -0.0 725,483 - LRK53_RS03105 0.19 -1.0 725,483 - LRK53_RS03105 0.19 -1.4 725,483 - LRK53_RS03105 0.19 +1.0 725,572 + LRK53_RS03105 0.33 -2.2 725,572 + LRK53_RS03105 0.33 -1.8 725,572 + LRK53_RS03105 0.33 +1.0 725,572 + LRK53_RS03105 0.33 -2.2 725,572 + LRK53_RS03105 0.33 -4.9 725,572 + LRK53_RS03105 0.33 -1.6 725,572 + LRK53_RS03105 0.33 -1.4 725,572 + LRK53_RS03105 0.33 +1.0 725,572 + LRK53_RS03105 0.33 -1.6 725,572 + LRK53_RS03105 0.33 -0.0 725,572 + LRK53_RS03105 0.33 -1.8 725,572 + LRK53_RS03105 0.33 -2.5 725,572 + LRK53_RS03105 0.33 -2.6 725,572 + LRK53_RS03105 0.33 +1.0 725,573 - LRK53_RS03105 0.33 -1.6 725,573 - LRK53_RS03105 0.33 -5.4 725,573 - LRK53_RS03105 0.33 -3.6 725,573 - LRK53_RS03105 0.33 -1.4 725,573 - LRK53_RS03105 0.33 -1.6 725,573 - LRK53_RS03105 0.33 -1.0 725,573 - LRK53_RS03105 0.33 -3.4 725,573 - LRK53_RS03105 0.33 -0.0 725,573 - LRK53_RS03105 0.33 -0.6 725,573 - LRK53_RS03105 0.33 -2.7 725,573 - LRK53_RS03105 0.33 -0.6 725,573 - LRK53_RS03105 0.33 -1.0 725,573 - LRK53_RS03105 0.33 -3.2 725,573 - LRK53_RS03105 0.33 -3.5 725,573 - LRK53_RS03105 0.33 -2.2 725,573 - LRK53_RS03105 0.33 -1.6 725,573 - LRK53_RS03105 0.33 -3.2 725,573 - LRK53_RS03105 0.33 -3.0 725,573 - LRK53_RS03105 0.33 -1.4 725,573 - LRK53_RS03105 0.33 -1.6 725,573 - LRK53_RS03105 0.33 -1.8 725,573 - LRK53_RS03105 0.33 -3.5 725,573 - LRK53_RS03105 0.33 -0.0 725,728 + LRK53_RS03105 0.57 -2.8 725,729 - LRK53_RS03105 0.57 -1.0 725,729 - LRK53_RS03105 0.57 -3.0 725,729 - LRK53_RS03105 0.57 -3.7 726,155 - -1.2 726,185 + -0.6 726,186 - -1.0 726,186 - +1.5 726,186 - +0.5 726,186 - -1.4 726,186 - -1.6 726,605 + LRK53_RS03110 0.31 -0.0 726,605 + LRK53_RS03110 0.31 -1.6 726,605 + LRK53_RS03110 0.31 -0.6 726,605 + LRK53_RS03110 0.31 -1.8 726,605 + LRK53_RS03110 0.31 -1.0 726,606 - LRK53_RS03110 0.31 -1.4 726,606 - LRK53_RS03110 0.31 -2.5 726,606 - LRK53_RS03110 0.31 -1.0 726,606 - LRK53_RS03110 0.31 -0.6 726,606 - LRK53_RS03110 0.31 -1.0 726,606 - LRK53_RS03110 0.31 -0.0 726,606 - LRK53_RS03110 0.31 -2.4 726,606 - LRK53_RS03110 0.31 -0.0 726,606 - LRK53_RS03110 0.31 -1.0 726,689 + LRK53_RS03110 0.37 -1.0 726,689 + LRK53_RS03110 0.37 -3.4 726,689 + LRK53_RS03110 0.37 -1.6 726,689 + LRK53_RS03110 0.37 -0.6 726,689 + LRK53_RS03110 0.37 +1.0 726,689 + LRK53_RS03110 0.37 -4.0 726,689 + LRK53_RS03110 0.37 -1.4 726,689 + LRK53_RS03110 0.37 -1.0 726,690 - LRK53_RS03110 0.37 -1.6 726,690 - LRK53_RS03110 0.37 -1.4 726,690 - LRK53_RS03110 0.37 -0.0 726,690 - LRK53_RS03110 0.37 -1.8 726,690 - LRK53_RS03110 0.37 -2.4 726,690 - LRK53_RS03110 0.37 -2.0 726,690 - LRK53_RS03110 0.37 -0.0 726,690 - LRK53_RS03110 0.37 -0.0 726,740 + LRK53_RS03110 0.41 +1.0 726,741 - LRK53_RS03110 0.41 -1.4 726,950 + LRK53_RS03110 0.56 -2.2 726,951 - LRK53_RS03110 0.56 -1.4 726,951 - LRK53_RS03110 0.56 -0.0 727,337 + LRK53_RS03110 0.83 -1.8 727,337 + LRK53_RS03110 0.83 -2.5 727,338 - LRK53_RS03110 0.83 -2.5 727,493 + -3.8 727,493 + -2.5 727,493 + -3.4 727,494 - -3.5 727,494 - -2.4 727,494 - +1.0 727,494 - +1.0 727,684 + LRK53_RS03115 0.11 -2.8 727,684 + LRK53_RS03115 0.11 -3.5 727,697 + LRK53_RS03115 0.12 +1.0 727,843 + LRK53_RS03115 0.27 -0.0 727,843 + LRK53_RS03115 0.27 -2.5 727,843 + LRK53_RS03115 0.27 -1.4 727,844 - LRK53_RS03115 0.27 +1.0 727,844 - LRK53_RS03115 0.27 -1.5 727,844 - LRK53_RS03115 0.27 +0.4 727,844 - LRK53_RS03115 0.27 -1.6 728,047 + LRK53_RS03115 0.47 -0.6 728,092 + LRK53_RS03115 0.52 -1.0 728,092 + LRK53_RS03115 0.52 -4.4 728,092 + LRK53_RS03115 0.52 -2.4 728,092 + LRK53_RS03115 0.52 -1.6 728,092 + LRK53_RS03115 0.52 -2.4 728,092 + LRK53_RS03115 0.52 -0.6 728,093 - LRK53_RS03115 0.52 -1.0 728,093 - LRK53_RS03115 0.52 -0.0 728,093 - LRK53_RS03115 0.52 -4.2 728,093 - LRK53_RS03115 0.52 -0.0 728,093 - LRK53_RS03115 0.52 -1.0 728,194 + LRK53_RS03115 0.62 -1.0 728,194 + LRK53_RS03115 0.62 -0.0 728,194 + LRK53_RS03115 0.62 -2.7 728,194 + LRK53_RS03115 0.62 -2.0 728,195 - LRK53_RS03115 0.62 -1.4 728,195 - LRK53_RS03115 0.62 -3.9 728,195 - LRK53_RS03115 0.62 -1.0 728,195 - LRK53_RS03115 0.62 -3.3 728,296 + LRK53_RS03115 0.72 -1.6 728,296 + LRK53_RS03115 0.72 +1.0 728,296 + LRK53_RS03115 0.72 -4.0 728,296 + LRK53_RS03115 0.72 -3.3 728,296 + LRK53_RS03115 0.72 -0.0 728,296 + LRK53_RS03115 0.72 -1.6 728,296 + LRK53_RS03115 0.72 -0.0 728,297 - LRK53_RS03115 0.72 -0.0 728,297 - LRK53_RS03115 0.72 -0.6 728,297 - LRK53_RS03115 0.72 -0.6 728,297 - LRK53_RS03115 0.72 -0.6 728,297 - LRK53_RS03115 0.72 -0.0 728,297 - LRK53_RS03115 0.72 -1.4 728,362 + LRK53_RS03115 0.79 -1.4 728,362 + LRK53_RS03115 0.79 -3.4 728,363 - LRK53_RS03115 0.79 -1.8 728,363 - LRK53_RS03115 0.79 -4.2 728,363 - LRK53_RS03115 0.79 -3.2 728,380 + LRK53_RS03115 0.81 -0.0 728,380 + LRK53_RS03115 0.81 +1.0 728,380 + LRK53_RS03115 0.81 +1.0 728,381 - LRK53_RS03115 0.81 -0.0 728,381 - LRK53_RS03115 0.81 -1.4 728,432 - LRK53_RS03115 0.86 -5.1 728,455 + LRK53_RS03115 0.88 +1.0 728,456 - LRK53_RS03115 0.88 -1.0 728,488 + -2.4 728,489 - -2.4 728,489 - -2.8
Or see this region's nucleotide sequence