Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18740 and LRK53_RS18745 are separated by 393 nucleotides LRK53_RS18745 and LRK53_RS18750 overlap by 20 nucleotides LRK53_RS18750 and LRK53_RS18755 are separated by 284 nucleotides LRK53_RS18755 and LRK53_RS18760 are separated by 59 nucleotides
LRK53_RS18740: LRK53_RS18740 - hypothetical protein, at 139,311 to 140,813
_RS18740
LRK53_RS18745: LRK53_RS18745 - hypothetical protein, at 141,207 to 141,458
_RS18745
LRK53_RS18750: LRK53_RS18750 - hypothetical protein, at 141,439 to 141,864
_RS18750
LRK53_RS18755: LRK53_RS18755 - hypothetical protein, at 142,149 to 142,334
_RS18755
LRK53_RS18760: LRK53_RS18760 - hypothetical protein, at 142,394 to 142,768
_RS18760
Position (kb)
141
142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 140.313 kb on + strand, within LRK53_RS18740 at 140.314 kb on - strand, within LRK53_RS18740 at 140.359 kb on + strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.440 kb on + strand, within LRK53_RS18740 at 140.440 kb on + strand, within LRK53_RS18740 at 140.441 kb on - strand, within LRK53_RS18740 at 140.462 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.486 kb on - strand, within LRK53_RS18740 at 140.735 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.820 kb on + strand at 140.820 kb on + strand at 140.821 kb on - strand at 140.821 kb on - strand at 140.821 kb on - strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.885 kb on - strand at 140.885 kb on - strand at 140.957 kb on + strand at 140.957 kb on + strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.428 kb on - strand, within LRK53_RS18745 at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.754 kb on + strand, within LRK53_RS18750 at 141.754 kb on + strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.772 kb on + strand, within LRK53_RS18750 at 141.772 kb on + strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.871 kb on - strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.896 kb on + strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.897 kb on - strand at 141.932 kb on - strand at 141.932 kb on - strand at 142.049 kb on + strand at 142.117 kb on + strand at 142.118 kb on - strand at 142.314 kb on + strand, within LRK53_RS18755 at 142.314 kb on + strand, within LRK53_RS18755 at 142.314 kb on + strand, within LRK53_RS18755 at 142.314 kb on + strand, within LRK53_RS18755 at 142.315 kb on - strand, within LRK53_RS18755 at 142.315 kb on - strand, within LRK53_RS18755 at 142.376 kb on - strand at 142.426 kb on - strand at 142.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 140,313 + LRK53_RS18740 0.67 +0.5 140,314 - LRK53_RS18740 0.67 -0.7 140,359 + LRK53_RS18740 0.70 +0.1 140,360 - LRK53_RS18740 0.70 -0.5 140,360 - LRK53_RS18740 0.70 -1.7 140,360 - LRK53_RS18740 0.70 -0.2 140,440 + LRK53_RS18740 0.75 +0.5 140,440 + LRK53_RS18740 0.75 -1.1 140,441 - LRK53_RS18740 0.75 -1.0 140,462 + LRK53_RS18740 0.77 -0.1 140,481 + LRK53_RS18740 0.78 -1.7 140,481 + LRK53_RS18740 0.78 -0.1 140,481 + LRK53_RS18740 0.78 -0.6 140,481 + LRK53_RS18740 0.78 -0.8 140,482 - LRK53_RS18740 0.78 -0.8 140,482 - LRK53_RS18740 0.78 -0.5 140,482 - LRK53_RS18740 0.78 +0.4 140,485 + LRK53_RS18740 0.78 -0.8 140,485 + LRK53_RS18740 0.78 -2.3 140,485 + LRK53_RS18740 0.78 -1.0 140,485 + LRK53_RS18740 0.78 +0.5 140,485 + LRK53_RS18740 0.78 -1.5 140,485 + LRK53_RS18740 0.78 -2.7 140,486 - LRK53_RS18740 0.78 -1.1 140,735 + -1.0 140,796 + +0.5 140,796 + -1.5 140,796 + -1.4 140,796 + +0.7 140,796 + -0.8 140,796 + -0.5 140,797 - +0.5 140,797 - -1.2 140,797 - -1.1 140,797 - -0.5 140,797 - -1.5 140,807 + +0.5 140,807 + -1.1 140,807 + -0.3 140,807 + -2.2 140,807 + -0.5 140,807 + -1.1 140,807 + -1.1 140,807 + +0.5 140,808 - +0.5 140,808 - -1.3 140,808 - -1.3 140,808 - -2.0 140,808 - -1.1 140,808 - +0.2 140,820 + -0.4 140,820 + +0.1 140,821 - -0.1 140,821 - -1.4 140,821 - -1.7 140,884 + -0.1 140,884 + +0.3 140,884 + -1.1 140,884 + -1.1 140,885 - -0.1 140,885 - -1.9 140,957 + -0.2 140,957 + -0.5 140,958 - +0.0 140,958 - +0.5 140,958 - +1.5 140,958 - +0.2 140,958 - -1.1 140,958 - -1.2 140,958 - -1.0 140,958 - -0.5 140,960 - +1.1 140,960 - -0.4 140,960 - +0.2 140,960 - +0.5 140,960 - -0.3 140,960 - -1.0 141,289 + LRK53_RS18745 0.33 -0.8 141,289 + LRK53_RS18745 0.33 +0.0 141,289 + LRK53_RS18745 0.33 +1.5 141,289 + LRK53_RS18745 0.33 -1.3 141,289 + LRK53_RS18745 0.33 -0.5 141,290 - LRK53_RS18745 0.33 -0.5 141,290 - LRK53_RS18745 0.33 +1.0 141,290 - LRK53_RS18745 0.33 +1.2 141,290 - LRK53_RS18745 0.33 -1.3 141,290 - LRK53_RS18745 0.33 -0.8 141,427 + LRK53_RS18745 0.87 +0.2 141,427 + LRK53_RS18745 0.87 -0.8 141,427 + LRK53_RS18745 0.87 +1.3 141,428 - LRK53_RS18745 0.88 -1.0 141,445 + -0.3 141,445 + -1.8 141,445 + -0.8 141,445 + -0.6 141,445 + -0.5 141,445 + +0.3 141,445 + +0.1 141,445 + -1.5 141,445 + -1.4 141,446 - -1.1 141,446 - -1.6 141,446 - -0.3 141,446 - -1.1 141,679 + LRK53_RS18750 0.56 -0.8 141,679 + LRK53_RS18750 0.56 -0.1 141,679 + LRK53_RS18750 0.56 +1.1 141,679 + LRK53_RS18750 0.56 -0.9 141,680 - LRK53_RS18750 0.57 -0.8 141,680 - LRK53_RS18750 0.57 -0.8 141,680 - LRK53_RS18750 0.57 +1.5 141,680 - LRK53_RS18750 0.57 -1.2 141,680 - LRK53_RS18750 0.57 +0.4 141,680 - LRK53_RS18750 0.57 -1.1 141,733 + LRK53_RS18750 0.69 +0.3 141,733 + LRK53_RS18750 0.69 -0.8 141,733 + LRK53_RS18750 0.69 -1.7 141,733 + LRK53_RS18750 0.69 -0.1 141,733 + LRK53_RS18750 0.69 -0.7 141,733 + LRK53_RS18750 0.69 -1.1 141,733 + LRK53_RS18750 0.69 -0.3 141,734 - LRK53_RS18750 0.69 -1.8 141,734 - LRK53_RS18750 0.69 -0.9 141,734 - LRK53_RS18750 0.69 -0.8 141,734 - LRK53_RS18750 0.69 -0.3 141,754 + LRK53_RS18750 0.74 +1.5 141,754 + LRK53_RS18750 0.74 +0.2 141,755 - LRK53_RS18750 0.74 +0.1 141,755 - LRK53_RS18750 0.74 -0.4 141,755 - LRK53_RS18750 0.74 -2.0 141,772 + LRK53_RS18750 0.78 +0.9 141,772 + LRK53_RS18750 0.78 -1.5 141,773 - LRK53_RS18750 0.78 +0.0 141,773 - LRK53_RS18750 0.78 -1.5 141,773 - LRK53_RS18750 0.78 +0.1 141,773 - LRK53_RS18750 0.78 +0.5 141,871 - -1.1 141,896 + +0.3 141,896 + -0.5 141,896 + -0.2 141,896 + +1.0 141,896 + +0.5 141,896 + -0.3 141,896 + -0.8 141,896 + +0.5 141,896 + -0.3 141,896 + -0.5 141,896 + -0.9 141,896 + -0.5 141,896 + -0.1 141,896 + -0.5 141,896 + -1.0 141,897 - -0.4 141,897 - -0.1 141,897 - +0.9 141,897 - -0.3 141,897 - -0.9 141,897 - -0.8 141,897 - -2.3 141,897 - -0.1 141,897 - +0.9 141,897 - -0.7 141,897 - -0.5 141,897 - -0.3 141,897 - -0.5 141,897 - -1.3 141,897 - -1.0 141,897 - -1.5 141,897 - -0.6 141,897 - -2.3 141,897 - -1.3 141,932 - +0.9 141,932 - +0.9 142,049 + +2.5 142,117 + -0.4 142,118 - -1.5 142,314 + LRK53_RS18755 0.89 -1.0 142,314 + LRK53_RS18755 0.89 +1.5 142,314 + LRK53_RS18755 0.89 +1.1 142,314 + LRK53_RS18755 0.89 -0.4 142,315 - LRK53_RS18755 0.89 -0.6 142,315 - LRK53_RS18755 0.89 -1.2 142,376 - -0.1 142,426 - +1.7 142,426 - -1.1
Or see this region's nucleotide sequence