Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16945 and LRK53_RS16950 overlap by 4 nucleotides LRK53_RS16950 and LRK53_RS16955 are separated by 57 nucleotides LRK53_RS16955 and LRK53_RS16960 are separated by 113 nucleotides
LRK53_RS16945: LRK53_RS16945 - serine/threonine-protein kinase, at 3,720,334 to 3,723,633
_RS16945
LRK53_RS16950: LRK53_RS16950 - sensor histidine kinase, at 3,723,630 to 3,724,361
_RS16950
LRK53_RS16955: LRK53_RS16955 - response regulator transcription factor, at 3,724,419 to 3,725,069
_RS16955
LRK53_RS16960: LRK53_RS16960 - 2OG-Fe(II) oxygenase, at 3,725,183 to 3,725,908
_RS16960
Position (kb)
3723
3724
3725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.713 kb on + strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.714 kb on - strand, within LRK53_RS16945 at 3722.724 kb on + strand, within LRK53_RS16945 at 3722.724 kb on + strand, within LRK53_RS16945 at 3722.725 kb on - strand, within LRK53_RS16945 at 3722.725 kb on - strand, within LRK53_RS16945 at 3722.725 kb on - strand, within LRK53_RS16945 at 3722.725 kb on - strand, within LRK53_RS16945 at 3723.091 kb on - strand, within LRK53_RS16945 at 3723.395 kb on + strand at 3723.395 kb on + strand at 3723.403 kb on + strand at 3723.624 kb on + strand at 3723.624 kb on + strand at 3723.624 kb on + strand at 3723.625 kb on - strand at 3723.625 kb on - strand at 3723.625 kb on - strand at 3724.147 kb on + strand, within LRK53_RS16950 at 3724.147 kb on + strand, within LRK53_RS16950 at 3724.147 kb on + strand, within LRK53_RS16950 at 3724.148 kb on - strand, within LRK53_RS16950 at 3724.148 kb on - strand, within LRK53_RS16950 at 3724.148 kb on - strand, within LRK53_RS16950 at 3724.148 kb on - strand, within LRK53_RS16950 at 3724.643 kb on - strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.695 kb on + strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.696 kb on - strand, within LRK53_RS16955 at 3724.731 kb on + strand, within LRK53_RS16955 at 3724.732 kb on - strand, within LRK53_RS16955 at 3724.775 kb on - strand, within LRK53_RS16955 at 3724.872 kb on + strand, within LRK53_RS16955 at 3724.996 kb on + strand, within LRK53_RS16955 at 3724.997 kb on - strand, within LRK53_RS16955 at 3724.997 kb on - strand, within LRK53_RS16955 at 3724.997 kb on - strand, within LRK53_RS16955 at 3724.997 kb on - strand, within LRK53_RS16955 at 3725.021 kb on + strand at 3725.041 kb on - strand at 3725.041 kb on - strand at 3725.041 kb on - strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.067 kb on + strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.068 kb on - strand at 3725.072 kb on - strand at 3725.097 kb on + strand at 3725.097 kb on + strand at 3725.234 kb on + strand at 3725.234 kb on + strand at 3725.234 kb on + strand at 3725.234 kb on + strand at 3725.235 kb on - strand at 3725.235 kb on - strand at 3725.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 3,722,713 + LRK53_RS16945 0.72 -1.6 3,722,713 + LRK53_RS16945 0.72 -2.4 3,722,713 + LRK53_RS16945 0.72 -0.1 3,722,713 + LRK53_RS16945 0.72 -0.2 3,722,713 + LRK53_RS16945 0.72 +0.6 3,722,713 + LRK53_RS16945 0.72 +0.2 3,722,713 + LRK53_RS16945 0.72 -0.5 3,722,713 + LRK53_RS16945 0.72 +0.7 3,722,713 + LRK53_RS16945 0.72 -0.6 3,722,713 + LRK53_RS16945 0.72 +2.2 3,722,713 + LRK53_RS16945 0.72 -1.1 3,722,714 - LRK53_RS16945 0.72 -1.2 3,722,714 - LRK53_RS16945 0.72 -1.0 3,722,714 - LRK53_RS16945 0.72 -0.8 3,722,714 - LRK53_RS16945 0.72 -0.4 3,722,714 - LRK53_RS16945 0.72 -0.4 3,722,714 - LRK53_RS16945 0.72 -0.2 3,722,714 - LRK53_RS16945 0.72 +0.1 3,722,724 + LRK53_RS16945 0.72 +0.6 3,722,724 + LRK53_RS16945 0.72 -0.6 3,722,725 - LRK53_RS16945 0.72 -1.1 3,722,725 - LRK53_RS16945 0.72 -0.8 3,722,725 - LRK53_RS16945 0.72 -2.4 3,722,725 - LRK53_RS16945 0.72 +0.4 3,723,091 - LRK53_RS16945 0.84 -1.0 3,723,395 + -1.4 3,723,395 + -0.1 3,723,403 + +0.6 3,723,624 + +1.2 3,723,624 + -0.6 3,723,624 + -0.5 3,723,625 - +2.2 3,723,625 - -1.6 3,723,625 - -1.0 3,724,147 + LRK53_RS16950 0.71 -1.3 3,724,147 + LRK53_RS16950 0.71 -1.2 3,724,147 + LRK53_RS16950 0.71 -0.1 3,724,148 - LRK53_RS16950 0.71 +0.2 3,724,148 - LRK53_RS16950 0.71 -0.8 3,724,148 - LRK53_RS16950 0.71 +0.2 3,724,148 - LRK53_RS16950 0.71 -1.7 3,724,643 - LRK53_RS16955 0.34 +0.2 3,724,695 + LRK53_RS16955 0.42 +1.2 3,724,695 + LRK53_RS16955 0.42 -1.3 3,724,695 + LRK53_RS16955 0.42 +2.2 3,724,695 + LRK53_RS16955 0.42 -1.8 3,724,695 + LRK53_RS16955 0.42 -1.2 3,724,695 + LRK53_RS16955 0.42 +2.2 3,724,695 + LRK53_RS16955 0.42 -0.0 3,724,695 + LRK53_RS16955 0.42 +0.2 3,724,695 + LRK53_RS16955 0.42 -0.0 3,724,695 + LRK53_RS16955 0.42 -1.1 3,724,695 + LRK53_RS16955 0.42 -0.3 3,724,696 - LRK53_RS16955 0.43 -1.2 3,724,696 - LRK53_RS16955 0.43 +0.2 3,724,696 - LRK53_RS16955 0.43 -0.4 3,724,696 - LRK53_RS16955 0.43 +0.2 3,724,696 - LRK53_RS16955 0.43 -1.2 3,724,696 - LRK53_RS16955 0.43 +1.2 3,724,696 - LRK53_RS16955 0.43 +0.2 3,724,696 - LRK53_RS16955 0.43 -0.4 3,724,696 - LRK53_RS16955 0.43 -2.4 3,724,696 - LRK53_RS16955 0.43 +0.8 3,724,696 - LRK53_RS16955 0.43 -1.4 3,724,731 + LRK53_RS16955 0.48 -0.8 3,724,732 - LRK53_RS16955 0.48 -0.3 3,724,775 - LRK53_RS16955 0.55 -0.2 3,724,872 + LRK53_RS16955 0.70 -0.7 3,724,996 + LRK53_RS16955 0.89 +2.2 3,724,997 - LRK53_RS16955 0.89 +1.2 3,724,997 - LRK53_RS16955 0.89 -0.2 3,724,997 - LRK53_RS16955 0.89 +0.2 3,724,997 - LRK53_RS16955 0.89 +0.2 3,725,021 + -2.2 3,725,041 - -0.8 3,725,041 - -0.4 3,725,041 - +0.0 3,725,067 + -1.6 3,725,067 + -0.4 3,725,067 + -1.2 3,725,067 + -0.8 3,725,067 + +1.2 3,725,067 + -1.1 3,725,067 + +1.2 3,725,067 + -1.2 3,725,067 + -0.3 3,725,067 + -0.4 3,725,068 - -2.3 3,725,068 - +0.6 3,725,068 - +1.2 3,725,068 - -1.2 3,725,068 - -2.8 3,725,068 - +2.2 3,725,068 - -2.3 3,725,068 - -0.8 3,725,068 - -0.4 3,725,072 - -0.3 3,725,097 + -0.5 3,725,097 + -0.2 3,725,234 + +0.6 3,725,234 + -1.8 3,725,234 + -0.8 3,725,234 + -0.2 3,725,235 - -0.6 3,725,235 - -2.0 3,725,235 - -0.3
Or see this region's nucleotide sequence