Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11515 and LRK53_RS11520 are separated by 44 nucleotides LRK53_RS11520 and LRK53_RS11525 are separated by 33 nucleotides LRK53_RS11525 and LRK53_RS11530 are separated by 71 nucleotides
LRK53_RS11515: LRK53_RS11515 - phosphoglucosamine mutase, at 2,561,017 to 2,562,369
_RS11515
LRK53_RS11520: LRK53_RS11520 - dihydropteroate synthase, at 2,562,414 to 2,563,328
_RS11520
LRK53_RS11525: LRK53_RS11525 - oligoribonuclease, at 2,563,362 to 2,563,928
_RS11525
LRK53_RS11530: LRK53_RS11530 - exodeoxyribonuclease I, at 2,564,000 to 2,565,454
_RS11530
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.322 kb on + strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.323 kb on - strand at 2563.954 kb on - strand at 2564.024 kb on + strand at 2564.024 kb on + strand at 2564.024 kb on + strand at 2564.024 kb on + strand at 2564.025 kb on - strand at 2564.025 kb on - strand at 2564.126 kb on + strand at 2564.126 kb on + strand at 2564.126 kb on + strand at 2564.126 kb on + strand at 2564.126 kb on + strand at 2564.126 kb on + strand at 2564.127 kb on - strand at 2564.127 kb on - strand at 2564.127 kb on - strand at 2564.127 kb on - strand at 2564.282 kb on + strand, within LRK53_RS11530 at 2564.282 kb on + strand, within LRK53_RS11530 at 2564.283 kb on - strand, within LRK53_RS11530 at 2564.328 kb on - strand, within LRK53_RS11530 at 2564.328 kb on - strand, within LRK53_RS11530
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 2,563,322 + +0.1 2,563,322 + -0.8 2,563,322 + -0.2 2,563,322 + -0.2 2,563,322 + -0.8 2,563,322 + +0.2 2,563,322 + -0.4 2,563,322 + +0.8 2,563,322 + +0.2 2,563,322 + -0.9 2,563,322 + -0.8 2,563,322 + +0.8 2,563,323 - -1.5 2,563,323 - -1.7 2,563,323 - +0.2 2,563,323 - -0.8 2,563,323 - -0.9 2,563,323 - -2.1 2,563,323 - -1.8 2,563,323 - -0.8 2,563,323 - -0.4 2,563,323 - +0.8 2,563,323 - -1.6 2,563,323 - +0.8 2,563,323 - -1.8 2,563,323 - -0.3 2,563,323 - -3.0 2,563,323 - +0.8 2,563,323 - -0.8 2,563,323 - -1.2 2,563,323 - -1.3 2,563,323 - -1.5 2,563,323 - -0.2 2,563,323 - -2.5 2,563,323 - +0.2 2,563,323 - -1.2 2,563,323 - -1.1 2,563,323 - -0.2 2,563,954 - -1.2 2,564,024 + +0.8 2,564,024 + -1.2 2,564,024 + -1.2 2,564,024 + -0.7 2,564,025 - -1.0 2,564,025 - -0.6 2,564,126 + -1.5 2,564,126 + -1.2 2,564,126 + +0.5 2,564,126 + -1.8 2,564,126 + -0.9 2,564,126 + -0.2 2,564,127 - -0.2 2,564,127 - -1.5 2,564,127 - -1.1 2,564,127 - -0.8 2,564,282 + LRK53_RS11530 0.19 -0.8 2,564,282 + LRK53_RS11530 0.19 +1.8 2,564,283 - LRK53_RS11530 0.19 -0.5 2,564,328 - LRK53_RS11530 0.23 -0.5 2,564,328 - LRK53_RS11530 0.23 +0.8
Or see this region's nucleotide sequence