Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08495 and LRK53_RS08500 are separated by 22 nucleotides LRK53_RS08500 and LRK53_RS08505 are separated by 47 nucleotides LRK53_RS08505 and LRK53_RS08510 overlap by 4 nucleotides LRK53_RS08510 and LRK53_RS08515 are separated by 165 nucleotides
LRK53_RS08495: LRK53_RS08495 - caspase family protein, at 1,907,277 to 1,908,281
_RS08495
LRK53_RS08500: LRK53_RS08500 - acireductone synthase, at 1,908,304 to 1,908,990
_RS08500
LRK53_RS08505: LRK53_RS08505 - acireductone dioxygenase, at 1,909,038 to 1,909,589
_RS08505
LRK53_RS08510: LRK53_RS08510 - methylthioribulose 1-phosphate dehydratase, at 1,909,586 to 1,910,236
_RS08510
LRK53_RS08515: LRK53_RS08515 - high-potential iron-sulfur protein, at 1,910,402 to 1,910,734
_RS08515
Position (kb)
1909
1910 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.079 kb on + strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.080 kb on - strand, within LRK53_RS08495 at 1908.275 kb on + strand at 1908.275 kb on + strand at 1908.275 kb on + strand at 1908.275 kb on + strand at 1908.275 kb on + strand at 1908.275 kb on + strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1908.276 kb on - strand at 1909.039 kb on + strand at 1909.039 kb on + strand at 1909.039 kb on + strand at 1909.039 kb on + strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.040 kb on - strand at 1909.141 kb on + strand, within LRK53_RS08505 at 1909.141 kb on + strand, within LRK53_RS08505 at 1909.141 kb on + strand, within LRK53_RS08505 at 1909.142 kb on - strand, within LRK53_RS08505 at 1909.142 kb on - strand, within LRK53_RS08505 at 1909.142 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.223 kb on - strand, within LRK53_RS08505 at 1909.379 kb on - strand, within LRK53_RS08505 at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.575 kb on - strand at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.686 kb on + strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.687 kb on - strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.710 kb on + strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.711 kb on - strand, within LRK53_RS08510 at 1909.825 kb on - strand, within LRK53_RS08510 at 1909.956 kb on + strand, within LRK53_RS08510 at 1909.956 kb on + strand, within LRK53_RS08510 at 1909.956 kb on + strand, within LRK53_RS08510 at 1909.956 kb on + strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1909.957 kb on - strand, within LRK53_RS08510 at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.266 kb on + strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.267 kb on - strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.268 kb on + strand at 1910.269 kb on - strand at 1910.269 kb on - strand at 1910.269 kb on - strand at 1910.269 kb on - strand at 1910.269 kb on - strand at 1910.296 kb on + strand at 1910.297 kb on - strand at 1910.343 kb on + strand at 1910.343 kb on + strand at 1910.343 kb on + strand at 1910.344 kb on - strand at 1910.344 kb on - strand at 1910.419 kb on + strand at 1910.419 kb on + strand at 1910.419 kb on + strand at 1910.420 kb on - strand at 1910.420 kb on - strand at 1910.420 kb on - strand at 1910.420 kb on - strand at 1910.561 kb on + strand, within LRK53_RS08515 at 1910.561 kb on + strand, within LRK53_RS08515 at 1910.562 kb on - strand, within LRK53_RS08515 at 1910.562 kb on - strand, within LRK53_RS08515 at 1910.562 kb on - strand, within LRK53_RS08515
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 1,908,079 + LRK53_RS08495 0.80 -0.7 1,908,079 + LRK53_RS08495 0.80 -0.2 1,908,079 + LRK53_RS08495 0.80 +0.3 1,908,079 + LRK53_RS08495 0.80 -1.1 1,908,079 + LRK53_RS08495 0.80 -0.1 1,908,079 + LRK53_RS08495 0.80 -0.2 1,908,080 - LRK53_RS08495 0.80 -0.8 1,908,080 - LRK53_RS08495 0.80 +0.0 1,908,080 - LRK53_RS08495 0.80 -1.6 1,908,080 - LRK53_RS08495 0.80 -0.8 1,908,080 - LRK53_RS08495 0.80 -1.2 1,908,080 - LRK53_RS08495 0.80 -0.6 1,908,080 - LRK53_RS08495 0.80 -0.7 1,908,275 + -0.6 1,908,275 + -0.8 1,908,275 + +0.3 1,908,275 + -0.6 1,908,275 + +0.8 1,908,275 + -0.7 1,908,276 - +0.8 1,908,276 - +1.8 1,908,276 - -1.9 1,908,276 - +1.8 1,908,276 - -1.0 1,908,276 - -0.6 1,908,276 - -0.9 1,908,276 - -0.7 1,908,276 - -2.2 1,908,276 - -0.4 1,908,276 - -0.2 1,908,276 - -0.2 1,909,039 + -0.8 1,909,039 + +0.8 1,909,039 + -0.2 1,909,039 + +0.2 1,909,040 - -2.4 1,909,040 - -0.2 1,909,040 - -1.6 1,909,040 - +0.8 1,909,040 - -0.2 1,909,040 - -2.9 1,909,040 - +0.8 1,909,141 + LRK53_RS08505 0.19 -4.2 1,909,141 + LRK53_RS08505 0.19 +0.8 1,909,141 + LRK53_RS08505 0.19 -0.2 1,909,142 - LRK53_RS08505 0.19 +0.8 1,909,142 - LRK53_RS08505 0.19 -0.8 1,909,142 - LRK53_RS08505 0.19 -1.7 1,909,223 - LRK53_RS08505 0.34 -2.7 1,909,223 - LRK53_RS08505 0.34 -1.6 1,909,223 - LRK53_RS08505 0.34 +0.8 1,909,223 - LRK53_RS08505 0.34 -1.2 1,909,223 - LRK53_RS08505 0.34 -2.0 1,909,223 - LRK53_RS08505 0.34 -2.4 1,909,379 - LRK53_RS08505 0.62 -3.1 1,909,575 - -0.8 1,909,575 - -1.6 1,909,575 - -0.9 1,909,575 - -2.4 1,909,575 - -3.3 1,909,575 - -0.9 1,909,575 - -0.8 1,909,686 + LRK53_RS08510 0.15 -0.6 1,909,686 + LRK53_RS08510 0.15 -0.2 1,909,686 + LRK53_RS08510 0.15 -2.0 1,909,686 + LRK53_RS08510 0.15 -1.8 1,909,686 + LRK53_RS08510 0.15 -0.8 1,909,686 + LRK53_RS08510 0.15 -0.8 1,909,686 + LRK53_RS08510 0.15 -2.2 1,909,686 + LRK53_RS08510 0.15 -1.3 1,909,686 + LRK53_RS08510 0.15 -1.8 1,909,686 + LRK53_RS08510 0.15 -0.7 1,909,686 + LRK53_RS08510 0.15 -0.8 1,909,686 + LRK53_RS08510 0.15 +0.8 1,909,686 + LRK53_RS08510 0.15 -0.6 1,909,686 + LRK53_RS08510 0.15 -0.8 1,909,686 + LRK53_RS08510 0.15 -1.6 1,909,686 + LRK53_RS08510 0.15 -2.8 1,909,686 + LRK53_RS08510 0.15 -1.6 1,909,687 - LRK53_RS08510 0.16 -1.9 1,909,687 - LRK53_RS08510 0.16 -2.7 1,909,687 - LRK53_RS08510 0.16 -0.2 1,909,687 - LRK53_RS08510 0.16 -0.8 1,909,687 - LRK53_RS08510 0.16 -1.9 1,909,687 - LRK53_RS08510 0.16 -0.8 1,909,687 - LRK53_RS08510 0.16 -1.6 1,909,687 - LRK53_RS08510 0.16 -0.6 1,909,687 - LRK53_RS08510 0.16 -0.6 1,909,687 - LRK53_RS08510 0.16 -2.1 1,909,687 - LRK53_RS08510 0.16 -2.2 1,909,687 - LRK53_RS08510 0.16 -1.1 1,909,687 - LRK53_RS08510 0.16 +0.3 1,909,687 - LRK53_RS08510 0.16 -2.2 1,909,687 - LRK53_RS08510 0.16 -2.4 1,909,687 - LRK53_RS08510 0.16 -1.0 1,909,687 - LRK53_RS08510 0.16 +0.8 1,909,687 - LRK53_RS08510 0.16 -2.7 1,909,687 - LRK53_RS08510 0.16 -1.7 1,909,687 - LRK53_RS08510 0.16 -1.9 1,909,687 - LRK53_RS08510 0.16 -0.2 1,909,687 - LRK53_RS08510 0.16 -0.2 1,909,687 - LRK53_RS08510 0.16 +0.8 1,909,710 + LRK53_RS08510 0.19 -0.8 1,909,710 + LRK53_RS08510 0.19 -2.0 1,909,710 + LRK53_RS08510 0.19 -2.0 1,909,710 + LRK53_RS08510 0.19 +0.8 1,909,710 + LRK53_RS08510 0.19 +0.8 1,909,710 + LRK53_RS08510 0.19 -2.3 1,909,711 - LRK53_RS08510 0.19 -1.8 1,909,711 - LRK53_RS08510 0.19 -0.8 1,909,711 - LRK53_RS08510 0.19 -2.2 1,909,711 - LRK53_RS08510 0.19 +0.0 1,909,711 - LRK53_RS08510 0.19 -2.2 1,909,711 - LRK53_RS08510 0.19 -2.0 1,909,825 - LRK53_RS08510 0.37 -2.4 1,909,956 + LRK53_RS08510 0.57 -0.8 1,909,956 + LRK53_RS08510 0.57 -0.2 1,909,956 + LRK53_RS08510 0.57 -1.9 1,909,956 + LRK53_RS08510 0.57 -0.7 1,909,957 - LRK53_RS08510 0.57 -2.2 1,909,957 - LRK53_RS08510 0.57 -2.8 1,909,957 - LRK53_RS08510 0.57 -0.8 1,909,957 - LRK53_RS08510 0.57 +0.4 1,909,957 - LRK53_RS08510 0.57 -1.6 1,909,957 - LRK53_RS08510 0.57 -1.9 1,909,957 - LRK53_RS08510 0.57 -1.8 1,909,957 - LRK53_RS08510 0.57 +0.8 1,909,957 - LRK53_RS08510 0.57 -0.6 1,910,266 + -1.8 1,910,266 + -2.0 1,910,266 + -1.2 1,910,266 + -1.2 1,910,266 + -1.0 1,910,266 + -1.4 1,910,266 + +0.2 1,910,267 - +0.8 1,910,267 - -1.2 1,910,267 - -1.2 1,910,267 - +0.2 1,910,267 - +0.8 1,910,267 - -1.5 1,910,267 - -0.0 1,910,267 - -0.7 1,910,268 + -0.8 1,910,268 + +1.8 1,910,268 + +0.2 1,910,268 + -0.4 1,910,268 + -0.2 1,910,268 + -0.7 1,910,268 + +1.8 1,910,268 + -0.7 1,910,268 + -1.6 1,910,268 + -0.5 1,910,268 + -0.2 1,910,268 + +0.2 1,910,268 + -1.8 1,910,268 + -0.4 1,910,268 + -0.2 1,910,269 - -0.8 1,910,269 - +1.3 1,910,269 - -0.5 1,910,269 - -0.1 1,910,269 - -0.5 1,910,296 + -0.6 1,910,297 - -0.1 1,910,343 + -0.2 1,910,343 + -1.8 1,910,343 + +0.2 1,910,344 - -0.8 1,910,344 - +0.3 1,910,419 + +0.4 1,910,419 + -0.5 1,910,419 + -1.8 1,910,420 - -0.2 1,910,420 - -0.6 1,910,420 - -0.8 1,910,420 - +0.8 1,910,561 + LRK53_RS08515 0.48 -1.4 1,910,561 + LRK53_RS08515 0.48 +0.2 1,910,562 - LRK53_RS08515 0.48 -0.2 1,910,562 - LRK53_RS08515 0.48 +0.8 1,910,562 - LRK53_RS08515 0.48 -0.7
Or see this region's nucleotide sequence