Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03155 and LRK53_RS03160 are separated by 164 nucleotides LRK53_RS03160 and LRK53_RS03165 overlap by 4 nucleotides
LRK53_RS03155: LRK53_RS03155 - threonine synthase, at 736,927 to 738,234
_RS03155
LRK53_RS03160: LRK53_RS03160 - homoserine kinase, at 738,399 to 739,397
_RS03160
LRK53_RS03165: LRK53_RS03165 - homoserine dehydrogenase, at 739,394 to 740,545
_RS03165
Position (kb) 738
739
740 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 737.410 kb on + strand, within LRK53_RS03155 at 737.410 kb on + strand, within LRK53_RS03155 at 737.410 kb on + strand, within LRK53_RS03155 at 737.411 kb on - strand, within LRK53_RS03155 at 737.411 kb on - strand, within LRK53_RS03155 at 737.540 kb on + strand, within LRK53_RS03155 at 737.541 kb on - strand, within LRK53_RS03155 at 737.541 kb on - strand, within LRK53_RS03155 at 737.543 kb on + strand, within LRK53_RS03155 at 737.543 kb on + strand, within LRK53_RS03155 at 737.543 kb on + strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 737.544 kb on - strand, within LRK53_RS03155 at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.134 kb on + strand at 738.135 kb on - strand at 738.135 kb on - strand at 738.135 kb on - strand at 738.135 kb on - strand at 738.135 kb on - strand at 738.135 kb on - strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.234 kb on + strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.235 kb on - strand at 738.315 kb on + strand at 738.315 kb on + strand at 738.315 kb on + strand at 738.370 kb on + strand at 738.371 kb on - strand at 738.391 kb on + strand at 738.392 kb on - strand at 738.547 kb on + strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.976 kb on + strand, within LRK53_RS03160 at 738.976 kb on + strand, within LRK53_RS03160 at 738.977 kb on - strand, within LRK53_RS03160 at 739.308 kb on + strand at 739.309 kb on - strand at 739.383 kb on + strand at 739.383 kb on + strand at 739.498 kb on + strand at 739.507 kb on - strand at 739.864 kb on - strand, within LRK53_RS03165 at 739.864 kb on - strand, within LRK53_RS03165 at 739.864 kb on - strand, within LRK53_RS03165 at 740.044 kb on - strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.164 kb on - strand, within LRK53_RS03165
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 737,410 + LRK53_RS03155 0.37 -3.4 737,410 + LRK53_RS03155 0.37 +1.0 737,410 + LRK53_RS03155 0.37 -2.9 737,411 - LRK53_RS03155 0.37 +1.0 737,411 - LRK53_RS03155 0.37 +1.0 737,540 + LRK53_RS03155 0.47 -0.0 737,541 - LRK53_RS03155 0.47 -4.8 737,541 - LRK53_RS03155 0.47 -1.0 737,543 + LRK53_RS03155 0.47 -1.0 737,543 + LRK53_RS03155 0.47 -3.7 737,543 + LRK53_RS03155 0.47 +1.0 737,544 - LRK53_RS03155 0.47 +1.0 737,544 - LRK53_RS03155 0.47 -1.6 737,544 - LRK53_RS03155 0.47 -2.9 737,544 - LRK53_RS03155 0.47 -3.6 737,544 - LRK53_RS03155 0.47 +1.0 737,544 - LRK53_RS03155 0.47 -2.3 737,544 - LRK53_RS03155 0.47 -0.0 737,544 - LRK53_RS03155 0.47 -2.2 738,134 + -0.0 738,134 + -2.2 738,134 + -1.8 738,134 + -1.3 738,134 + -3.0 738,134 + -0.6 738,134 + -0.0 738,134 + -2.8 738,135 - -1.6 738,135 - -1.3 738,135 - -0.6 738,135 - -0.6 738,135 - -1.6 738,135 - -0.6 738,234 + -1.5 738,234 + -0.6 738,234 + +2.0 738,234 + -0.3 738,234 + +0.6 738,234 + -1.0 738,234 + -0.0 738,234 + -0.0 738,234 + -0.0 738,234 + -1.8 738,234 + -1.5 738,234 + +1.0 738,234 + -1.1 738,234 + -2.3 738,234 + -1.3 738,234 + -0.0 738,235 - -0.5 738,235 - -1.0 738,235 - +0.4 738,235 - -2.9 738,235 - -0.0 738,235 - -0.3 738,235 - -0.2 738,235 - -0.0 738,235 - -1.4 738,235 - +2.0 738,235 - +0.4 738,235 - -1.1 738,235 - -2.0 738,235 - -1.8 738,235 - -1.0 738,235 - -0.0 738,235 - -2.2 738,235 - -0.0 738,235 - -0.1 738,235 - +0.4 738,315 + -0.8 738,315 + -1.6 738,315 + -0.0 738,370 + -0.8 738,371 - +1.0 738,391 + -1.8 738,392 - -0.0 738,547 + LRK53_RS03160 0.15 -0.6 738,548 - LRK53_RS03160 0.15 -1.0 738,548 - LRK53_RS03160 0.15 -3.0 738,548 - LRK53_RS03160 0.15 -2.5 738,976 + LRK53_RS03160 0.58 -1.6 738,976 + LRK53_RS03160 0.58 -3.1 738,977 - LRK53_RS03160 0.58 -0.6 739,308 + -1.9 739,309 - -2.3 739,383 + +1.0 739,383 + -0.0 739,498 + -1.2 739,507 - -1.0 739,864 - LRK53_RS03165 0.41 -2.3 739,864 - LRK53_RS03165 0.41 -2.5 739,864 - LRK53_RS03165 0.41 -1.0 740,044 - LRK53_RS03165 0.56 -0.8 740,163 + LRK53_RS03165 0.67 -2.0 740,163 + LRK53_RS03165 0.67 -0.6 740,163 + LRK53_RS03165 0.67 -1.0 740,164 - LRK53_RS03165 0.67 -0.6
Or see this region's nucleotide sequence