Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03070 and LRK53_RS03075 are separated by 227 nucleotides LRK53_RS03075 and LRK53_RS03080 overlap by 4 nucleotides LRK53_RS03080 and LRK53_RS03085 are separated by 166 nucleotides
LRK53_RS03070: LRK53_RS03070 - GGDEF domain-containing protein, at 715,847 to 716,983
_RS03070
LRK53_RS03075: LRK53_RS03075 - threonine ammonia-lyase, biosynthetic, at 717,211 to 718,761
_RS03075
LRK53_RS03080: LRK53_RS03080 - acetolactate synthase small subunit, at 718,758 to 719,006
_RS03080
LRK53_RS03085: LRK53_RS03085 - biosynthetic-type acetolactate synthase large subunit, at 719,173 to 721,029
_RS03085
Position (kb)
717
718
719 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 716.366 kb on + strand, within LRK53_RS03070 at 716.366 kb on + strand, within LRK53_RS03070 at 716.367 kb on - strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.375 kb on + strand, within LRK53_RS03070 at 716.376 kb on - strand, within LRK53_RS03070 at 716.376 kb on - strand, within LRK53_RS03070 at 716.376 kb on - strand, within LRK53_RS03070 at 716.376 kb on - strand, within LRK53_RS03070 at 716.376 kb on - strand, within LRK53_RS03070 at 716.423 kb on + strand, within LRK53_RS03070 at 716.423 kb on + strand, within LRK53_RS03070 at 716.423 kb on + strand, within LRK53_RS03070 at 716.424 kb on - strand, within LRK53_RS03070 at 716.424 kb on - strand, within LRK53_RS03070 at 716.587 kb on + strand, within LRK53_RS03070 at 716.587 kb on + strand, within LRK53_RS03070 at 716.587 kb on + strand, within LRK53_RS03070 at 716.587 kb on + strand, within LRK53_RS03070 at 716.588 kb on - strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.591 kb on + strand, within LRK53_RS03070 at 716.592 kb on - strand, within LRK53_RS03070 at 716.592 kb on - strand, within LRK53_RS03070 at 716.592 kb on - strand, within LRK53_RS03070 at 716.592 kb on - strand, within LRK53_RS03070 at 716.592 kb on - strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.603 kb on + strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.604 kb on - strand, within LRK53_RS03070 at 716.838 kb on - strand, within LRK53_RS03070 at 716.876 kb on + strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.877 kb on - strand at 716.879 kb on + strand at 716.879 kb on + strand at 716.880 kb on - strand at 716.880 kb on - strand at 716.995 kb on + strand at 716.996 kb on - strand at 717.452 kb on + strand, within LRK53_RS03075 at 717.453 kb on - strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.650 kb on + strand, within LRK53_RS03075 at 717.651 kb on - strand, within LRK53_RS03075 at 717.651 kb on - strand, within LRK53_RS03075 at 717.651 kb on - strand, within LRK53_RS03075 at 717.651 kb on - strand, within LRK53_RS03075 at 717.857 kb on + strand, within LRK53_RS03075 at 717.857 kb on + strand, within LRK53_RS03075 at 717.858 kb on - strand, within LRK53_RS03075 at 717.858 kb on - strand, within LRK53_RS03075 at 717.980 kb on + strand, within LRK53_RS03075 at 717.980 kb on + strand, within LRK53_RS03075 at 717.980 kb on + strand, within LRK53_RS03075 at 717.980 kb on + strand, within LRK53_RS03075 at 717.980 kb on + strand, within LRK53_RS03075 at 717.981 kb on - strand, within LRK53_RS03075 at 717.981 kb on - strand, within LRK53_RS03075 at 717.981 kb on - strand, within LRK53_RS03075 at 717.981 kb on - strand, within LRK53_RS03075 at 717.981 kb on - strand, within LRK53_RS03075 at 718.352 kb on + strand, within LRK53_RS03075 at 718.352 kb on + strand, within LRK53_RS03075 at 718.353 kb on - strand, within LRK53_RS03075 at 718.353 kb on - strand, within LRK53_RS03075 at 718.565 kb on + strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.566 kb on - strand, within LRK53_RS03075 at 718.688 kb on + strand at 718.688 kb on + strand at 718.688 kb on + strand at 718.688 kb on + strand at 718.689 kb on - strand at 718.689 kb on - strand at 718.689 kb on - strand at 718.924 kb on + strand, within LRK53_RS03080 at 718.925 kb on - strand, within LRK53_RS03080 at 719.113 kb on - strand at 719.255 kb on + strand at 719.255 kb on + strand at 719.256 kb on - strand at 719.256 kb on - strand at 719.283 kb on - strand at 719.477 kb on + strand, within LRK53_RS03085 at 719.477 kb on + strand, within LRK53_RS03085 at 719.477 kb on + strand, within LRK53_RS03085 at 719.477 kb on + strand, within LRK53_RS03085 at 719.477 kb on + strand, within LRK53_RS03085 at 719.477 kb on + strand, within LRK53_RS03085 at 719.478 kb on - strand, within LRK53_RS03085 at 719.478 kb on - strand, within LRK53_RS03085 at 719.478 kb on - strand, within LRK53_RS03085 at 719.550 kb on - strand, within LRK53_RS03085 at 719.550 kb on - strand, within LRK53_RS03085 at 719.726 kb on + strand, within LRK53_RS03085 at 719.726 kb on + strand, within LRK53_RS03085 at 719.726 kb on + strand, within LRK53_RS03085 at 719.726 kb on + strand, within LRK53_RS03085 at 719.726 kb on + strand, within LRK53_RS03085 at 719.727 kb on - strand, within LRK53_RS03085 at 719.727 kb on - strand, within LRK53_RS03085 at 719.727 kb on - strand, within LRK53_RS03085 at 719.727 kb on - strand, within LRK53_RS03085
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 716,366 + LRK53_RS03070 0.46 -1.0 716,366 + LRK53_RS03070 0.46 -1.7 716,367 - LRK53_RS03070 0.46 -1.5 716,375 + LRK53_RS03070 0.46 -1.1 716,375 + LRK53_RS03070 0.46 -0.4 716,375 + LRK53_RS03070 0.46 -0.6 716,375 + LRK53_RS03070 0.46 -0.7 716,375 + LRK53_RS03070 0.46 +1.0 716,375 + LRK53_RS03070 0.46 +1.2 716,375 + LRK53_RS03070 0.46 +0.1 716,375 + LRK53_RS03070 0.46 -1.0 716,375 + LRK53_RS03070 0.46 -0.9 716,375 + LRK53_RS03070 0.46 -1.0 716,375 + LRK53_RS03070 0.46 -0.2 716,376 - LRK53_RS03070 0.47 -0.5 716,376 - LRK53_RS03070 0.47 -1.8 716,376 - LRK53_RS03070 0.47 -1.5 716,376 - LRK53_RS03070 0.47 +2.6 716,376 - LRK53_RS03070 0.47 -1.8 716,423 + LRK53_RS03070 0.51 -0.3 716,423 + LRK53_RS03070 0.51 -0.2 716,423 + LRK53_RS03070 0.51 +0.5 716,424 - LRK53_RS03070 0.51 +1.0 716,424 - LRK53_RS03070 0.51 -1.0 716,587 + LRK53_RS03070 0.65 -1.3 716,587 + LRK53_RS03070 0.65 +0.5 716,587 + LRK53_RS03070 0.65 -0.6 716,587 + LRK53_RS03070 0.65 -0.6 716,588 - LRK53_RS03070 0.65 -1.1 716,591 + LRK53_RS03070 0.65 -0.0 716,591 + LRK53_RS03070 0.65 +1.0 716,591 + LRK53_RS03070 0.65 +0.3 716,591 + LRK53_RS03070 0.65 +0.4 716,591 + LRK53_RS03070 0.65 -1.6 716,591 + LRK53_RS03070 0.65 +1.0 716,592 - LRK53_RS03070 0.66 -0.0 716,592 - LRK53_RS03070 0.66 -0.1 716,592 - LRK53_RS03070 0.66 -0.6 716,592 - LRK53_RS03070 0.66 -0.2 716,592 - LRK53_RS03070 0.66 -0.5 716,603 + LRK53_RS03070 0.66 -2.5 716,603 + LRK53_RS03070 0.66 -0.6 716,603 + LRK53_RS03070 0.66 -0.3 716,603 + LRK53_RS03070 0.66 -1.8 716,603 + LRK53_RS03070 0.66 +1.0 716,603 + LRK53_RS03070 0.66 +0.6 716,604 - LRK53_RS03070 0.67 -0.6 716,604 - LRK53_RS03070 0.67 +0.4 716,604 - LRK53_RS03070 0.67 +0.4 716,604 - LRK53_RS03070 0.67 -2.5 716,604 - LRK53_RS03070 0.67 -0.6 716,604 - LRK53_RS03070 0.67 -1.0 716,838 - LRK53_RS03070 0.87 -0.8 716,876 + -0.3 716,877 - +0.6 716,877 - -1.3 716,877 - +1.0 716,877 - -0.6 716,877 - -1.0 716,877 - -0.8 716,877 - -1.2 716,879 + -1.0 716,879 + -1.2 716,880 - -1.6 716,880 - -0.6 716,995 + -1.3 716,996 - +0.3 717,452 + LRK53_RS03075 0.16 -1.3 717,453 - LRK53_RS03075 0.16 -0.6 717,650 + LRK53_RS03075 0.28 -0.0 717,650 + LRK53_RS03075 0.28 -1.3 717,650 + LRK53_RS03075 0.28 -2.3 717,650 + LRK53_RS03075 0.28 -1.3 717,650 + LRK53_RS03075 0.28 +2.0 717,650 + LRK53_RS03075 0.28 -0.0 717,651 - LRK53_RS03075 0.28 -0.6 717,651 - LRK53_RS03075 0.28 -3.6 717,651 - LRK53_RS03075 0.28 -2.2 717,651 - LRK53_RS03075 0.28 +1.0 717,857 + LRK53_RS03075 0.42 -2.6 717,857 + LRK53_RS03075 0.42 -1.6 717,858 - LRK53_RS03075 0.42 -1.6 717,858 - LRK53_RS03075 0.42 -0.9 717,980 + LRK53_RS03075 0.50 -1.2 717,980 + LRK53_RS03075 0.50 -1.8 717,980 + LRK53_RS03075 0.50 -0.6 717,980 + LRK53_RS03075 0.50 -0.0 717,980 + LRK53_RS03075 0.50 -0.0 717,981 - LRK53_RS03075 0.50 -3.3 717,981 - LRK53_RS03075 0.50 -1.0 717,981 - LRK53_RS03075 0.50 -1.3 717,981 - LRK53_RS03075 0.50 -0.0 717,981 - LRK53_RS03075 0.50 -3.7 718,352 + LRK53_RS03075 0.74 -1.3 718,352 + LRK53_RS03075 0.74 -1.8 718,353 - LRK53_RS03075 0.74 -1.8 718,353 - LRK53_RS03075 0.74 +0.4 718,565 + LRK53_RS03075 0.87 -1.6 718,566 - LRK53_RS03075 0.87 -2.2 718,566 - LRK53_RS03075 0.87 -0.6 718,566 - LRK53_RS03075 0.87 -0.6 718,566 - LRK53_RS03075 0.87 -2.9 718,566 - LRK53_RS03075 0.87 -0.6 718,566 - LRK53_RS03075 0.87 -2.3 718,566 - LRK53_RS03075 0.87 -0.0 718,688 + -1.3 718,688 + -1.3 718,688 + -2.2 718,688 + -2.9 718,689 - -3.1 718,689 - -2.3 718,689 - -1.5 718,924 + LRK53_RS03080 0.67 -2.0 718,925 - LRK53_RS03080 0.67 -0.0 719,113 - -1.2 719,255 + -0.0 719,255 + -2.3 719,256 - -0.0 719,256 - -2.7 719,283 - -3.2 719,477 + LRK53_RS03085 0.16 -0.6 719,477 + LRK53_RS03085 0.16 -2.0 719,477 + LRK53_RS03085 0.16 -1.0 719,477 + LRK53_RS03085 0.16 -1.0 719,477 + LRK53_RS03085 0.16 -0.6 719,477 + LRK53_RS03085 0.16 +1.0 719,478 - LRK53_RS03085 0.16 -0.6 719,478 - LRK53_RS03085 0.16 +1.0 719,478 - LRK53_RS03085 0.16 -1.6 719,550 - LRK53_RS03085 0.20 +1.0 719,550 - LRK53_RS03085 0.20 -1.0 719,726 + LRK53_RS03085 0.30 -1.0 719,726 + LRK53_RS03085 0.30 -3.8 719,726 + LRK53_RS03085 0.30 -2.6 719,726 + LRK53_RS03085 0.30 -1.0 719,726 + LRK53_RS03085 0.30 -1.6 719,727 - LRK53_RS03085 0.30 -2.3 719,727 - LRK53_RS03085 0.30 -1.6 719,727 - LRK53_RS03085 0.30 -0.0 719,727 - LRK53_RS03085 0.30 -3.3
Or see this region's nucleotide sequence