Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02835 and LRK53_RS02840 are separated by 95 nucleotides LRK53_RS02840 and LRK53_RS02845 are separated by 13 nucleotides LRK53_RS02845 and LRK53_RS02850 are separated by 336 nucleotides
LRK53_RS02835: LRK53_RS02835 - ATP-dependent RNA helicase DbpA, at 657,460 to 658,839
_RS02835
LRK53_RS02840: LRK53_RS02840 - dihydroneopterin aldolase, at 658,935 to 659,291
_RS02840
LRK53_RS02845: LRK53_RS02845 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 659,305 to 659,793
_RS02845
LRK53_RS02850: LRK53_RS02850 - response regulator, at 660,130 to 660,564
_RS02850
Position (kb)
658
659
660 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 658.043 kb on + strand, within LRK53_RS02835 at 658.043 kb on + strand, within LRK53_RS02835 at 658.283 kb on + strand, within LRK53_RS02835 at 658.283 kb on + strand, within LRK53_RS02835 at 658.283 kb on + strand, within LRK53_RS02835 at 658.284 kb on - strand, within LRK53_RS02835 at 658.284 kb on - strand, within LRK53_RS02835 at 658.775 kb on + strand at 658.907 kb on + strand at 658.907 kb on + strand at 658.907 kb on + strand at 658.907 kb on + strand at 659.290 kb on - strand at 659.318 kb on - strand at 659.353 kb on + strand at 659.417 kb on - strand, within LRK53_RS02845 at 659.596 kb on + strand, within LRK53_RS02845 at 659.597 kb on - strand, within LRK53_RS02845 at 659.597 kb on - strand, within LRK53_RS02845 at 659.597 kb on - strand, within LRK53_RS02845 at 659.597 kb on - strand, within LRK53_RS02845 at 660.217 kb on + strand, within LRK53_RS02850 at 660.217 kb on + strand, within LRK53_RS02850 at 660.218 kb on - strand, within LRK53_RS02850
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 658,043 + LRK53_RS02835 0.42 -0.6 658,043 + LRK53_RS02835 0.42 -0.6 658,283 + LRK53_RS02835 0.60 -0.0 658,283 + LRK53_RS02835 0.60 +0.6 658,283 + LRK53_RS02835 0.60 -1.0 658,284 - LRK53_RS02835 0.60 -0.8 658,284 - LRK53_RS02835 0.60 -0.2 658,775 + -1.6 658,907 + -1.2 658,907 + +0.6 658,907 + -1.2 658,907 + -1.3 659,290 - -1.8 659,318 - -1.8 659,353 + -1.8 659,417 - LRK53_RS02845 0.23 +2.0 659,596 + LRK53_RS02845 0.60 -1.1 659,597 - LRK53_RS02845 0.60 -1.9 659,597 - LRK53_RS02845 0.60 -1.0 659,597 - LRK53_RS02845 0.60 -1.0 659,597 - LRK53_RS02845 0.60 -1.7 660,217 + LRK53_RS02850 0.20 +1.0 660,217 + LRK53_RS02850 0.20 +2.0 660,218 - LRK53_RS02850 0.20 +0.4
Or see this region's nucleotide sequence