Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02005 and LRK53_RS02010 are separated by 301 nucleotides LRK53_RS02010 and LRK53_RS02015 are separated by 263 nucleotides
LRK53_RS02005: LRK53_RS02005 - DISARM system phospholipase D-like protein DrmC, at 472,169 to 474,790
_RS02005
LRK53_RS02010: LRK53_RS02010 - DNA repair protein RadC, at 475,092 to 475,577
_RS02010
LRK53_RS02015: LRK53_RS02015 - HEPN domain-containing protein, at 475,841 to 476,482
_RS02015
Position (kb) 475
476 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 474.292 kb on + strand, within LRK53_RS02005 at 474.292 kb on + strand, within LRK53_RS02005 at 474.292 kb on + strand, within LRK53_RS02005 at 474.292 kb on + strand, within LRK53_RS02005 at 474.292 kb on + strand, within LRK53_RS02005 at 474.293 kb on - strand, within LRK53_RS02005 at 474.293 kb on - strand, within LRK53_RS02005 at 474.300 kb on + strand, within LRK53_RS02005 at 474.301 kb on - strand, within LRK53_RS02005 at 474.301 kb on - strand, within LRK53_RS02005 at 474.301 kb on - strand, within LRK53_RS02005 at 474.366 kb on + strand, within LRK53_RS02005 at 474.366 kb on + strand, within LRK53_RS02005 at 474.366 kb on + strand, within LRK53_RS02005 at 474.366 kb on + strand, within LRK53_RS02005 at 474.367 kb on - strand, within LRK53_RS02005 at 474.367 kb on - strand, within LRK53_RS02005 at 474.367 kb on - strand, within LRK53_RS02005 at 474.367 kb on - strand, within LRK53_RS02005 at 474.367 kb on - strand, within LRK53_RS02005 at 474.399 kb on + strand, within LRK53_RS02005 at 474.400 kb on - strand, within LRK53_RS02005 at 474.413 kb on + strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.414 kb on - strand, within LRK53_RS02005 at 474.480 kb on + strand, within LRK53_RS02005 at 474.480 kb on + strand, within LRK53_RS02005 at 474.480 kb on + strand, within LRK53_RS02005 at 474.481 kb on - strand, within LRK53_RS02005 at 474.481 kb on - strand, within LRK53_RS02005 at 474.481 kb on - strand, within LRK53_RS02005 at 474.481 kb on - strand, within LRK53_RS02005 at 474.481 kb on - strand, within LRK53_RS02005 at 474.566 kb on + strand at 474.566 kb on + strand at 474.566 kb on + strand at 474.567 kb on - strand at 474.767 kb on - strand at 474.774 kb on + strand at 474.774 kb on + strand at 474.774 kb on + strand at 474.775 kb on - strand at 474.775 kb on - strand at 474.775 kb on - strand at 474.786 kb on + strand at 474.786 kb on + strand at 474.786 kb on + strand at 474.786 kb on + strand at 474.806 kb on + strand at 474.806 kb on + strand at 474.807 kb on - strand at 474.807 kb on - strand at 474.870 kb on + strand at 474.922 kb on + strand at 474.922 kb on + strand at 474.923 kb on - strand at 474.923 kb on - strand at 474.923 kb on - strand at 474.923 kb on - strand at 474.923 kb on - strand at 474.923 kb on - strand at 474.923 kb on - strand at 475.028 kb on + strand at 475.034 kb on + strand at 475.035 kb on - strand at 475.035 kb on - strand at 475.035 kb on - strand at 475.060 kb on + strand at 475.061 kb on - strand at 475.098 kb on - strand at 475.116 kb on + strand at 475.116 kb on + strand at 475.117 kb on - strand at 475.117 kb on - strand at 475.117 kb on - strand at 475.117 kb on - strand at 475.125 kb on + strand at 475.159 kb on + strand, within LRK53_RS02010 at 475.160 kb on - strand, within LRK53_RS02010 at 475.160 kb on - strand, within LRK53_RS02010 at 475.160 kb on - strand, within LRK53_RS02010 at 475.179 kb on - strand, within LRK53_RS02010 at 475.179 kb on - strand, within LRK53_RS02010 at 475.179 kb on - strand, within LRK53_RS02010 at 475.378 kb on + strand, within LRK53_RS02010 at 475.378 kb on + strand, within LRK53_RS02010 at 475.378 kb on + strand, within LRK53_RS02010 at 475.378 kb on + strand, within LRK53_RS02010 at 475.379 kb on - strand, within LRK53_RS02010 at 475.379 kb on - strand, within LRK53_RS02010 at 475.379 kb on - strand, within LRK53_RS02010 at 475.379 kb on - strand, within LRK53_RS02010 at 475.379 kb on - strand, within LRK53_RS02010 at 475.441 kb on + strand, within LRK53_RS02010 at 475.441 kb on + strand, within LRK53_RS02010 at 475.442 kb on - strand, within LRK53_RS02010 at 475.442 kb on - strand, within LRK53_RS02010 at 475.442 kb on - strand, within LRK53_RS02010 at 475.515 kb on - strand, within LRK53_RS02010 at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on + strand at 475.573 kb on - strand at 475.574 kb on - strand at 475.574 kb on - strand at 475.695 kb on + strand at 475.696 kb on - strand at 475.696 kb on - strand at 475.696 kb on - strand at 475.797 kb on + strand at 475.797 kb on + strand at 475.797 kb on + strand at 475.797 kb on + strand at 475.798 kb on - strand at 475.798 kb on - strand at 475.798 kb on - strand at 475.798 kb on - strand at 475.798 kb on - strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.806 kb on + strand at 475.807 kb on - strand at 475.807 kb on - strand at 475.807 kb on - strand at 475.807 kb on - strand at 475.807 kb on - strand at 475.823 kb on + strand at 475.823 kb on + strand at 475.823 kb on + strand at 475.824 kb on - strand at 475.824 kb on - strand at 475.824 kb on - strand at 475.976 kb on + strand, within LRK53_RS02015 at 475.976 kb on + strand, within LRK53_RS02015 at 475.976 kb on + strand, within LRK53_RS02015 at 475.976 kb on + strand, within LRK53_RS02015 at 475.977 kb on - strand, within LRK53_RS02015 at 475.977 kb on - strand, within LRK53_RS02015 at 475.977 kb on - strand, within LRK53_RS02015 at 475.978 kb on + strand, within LRK53_RS02015 at 475.978 kb on + strand, within LRK53_RS02015 at 475.978 kb on + strand, within LRK53_RS02015 at 475.978 kb on + strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 475.979 kb on - strand, within LRK53_RS02015 at 476.009 kb on + strand, within LRK53_RS02015 at 476.010 kb on - strand, within LRK53_RS02015 at 476.010 kb on - strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.052 kb on + strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.053 kb on - strand, within LRK53_RS02015 at 476.112 kb on + strand, within LRK53_RS02015 at 476.112 kb on + strand, within LRK53_RS02015 at 476.112 kb on + strand, within LRK53_RS02015 at 476.112 kb on + strand, within LRK53_RS02015 at 476.113 kb on - strand, within LRK53_RS02015 at 476.134 kb on + strand, within LRK53_RS02015 at 476.134 kb on + strand, within LRK53_RS02015 at 476.134 kb on + strand, within LRK53_RS02015 at 476.135 kb on - strand, within LRK53_RS02015 at 476.204 kb on - strand, within LRK53_RS02015 at 476.204 kb on - strand, within LRK53_RS02015 at 476.204 kb on - strand, within LRK53_RS02015 at 476.230 kb on - strand, within LRK53_RS02015 at 476.309 kb on + strand, within LRK53_RS02015 at 476.310 kb on - strand, within LRK53_RS02015 at 476.310 kb on - strand, within LRK53_RS02015 at 476.310 kb on - strand, within LRK53_RS02015 at 476.461 kb on + strand at 476.461 kb on + strand at 476.462 kb on - strand at 476.462 kb on - strand at 476.462 kb on - strand at 476.462 kb on - strand at 476.462 kb on - strand at 476.462 kb on - strand at 476.480 kb on + strand at 476.532 kb on + strand at 476.541 kb on + strand at 476.541 kb on + strand at 476.541 kb on + strand at 476.541 kb on + strand at 476.541 kb on + strand at 476.541 kb on + strand at 476.542 kb on - strand at 476.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 474,292 + LRK53_RS02005 0.81 -1.8 474,292 + LRK53_RS02005 0.81 -1.1 474,292 + LRK53_RS02005 0.81 -0.5 474,292 + LRK53_RS02005 0.81 -1.7 474,292 + LRK53_RS02005 0.81 +0.6 474,293 - LRK53_RS02005 0.81 -1.2 474,293 - LRK53_RS02005 0.81 +1.1 474,300 + LRK53_RS02005 0.81 -0.9 474,301 - LRK53_RS02005 0.81 -0.8 474,301 - LRK53_RS02005 0.81 +0.1 474,301 - LRK53_RS02005 0.81 +1.5 474,366 + LRK53_RS02005 0.84 -1.2 474,366 + LRK53_RS02005 0.84 -2.3 474,366 + LRK53_RS02005 0.84 -0.9 474,366 + LRK53_RS02005 0.84 -2.2 474,367 - LRK53_RS02005 0.84 +0.7 474,367 - LRK53_RS02005 0.84 -1.8 474,367 - LRK53_RS02005 0.84 +0.1 474,367 - LRK53_RS02005 0.84 -0.5 474,367 - LRK53_RS02005 0.84 -2.1 474,399 + LRK53_RS02005 0.85 -1.2 474,400 - LRK53_RS02005 0.85 +1.1 474,413 + LRK53_RS02005 0.86 +0.7 474,414 - LRK53_RS02005 0.86 -1.2 474,414 - LRK53_RS02005 0.86 -0.2 474,414 - LRK53_RS02005 0.86 +0.1 474,414 - LRK53_RS02005 0.86 -1.9 474,414 - LRK53_RS02005 0.86 -0.9 474,414 - LRK53_RS02005 0.86 +1.1 474,480 + LRK53_RS02005 0.88 -1.5 474,480 + LRK53_RS02005 0.88 -0.1 474,480 + LRK53_RS02005 0.88 -0.9 474,481 - LRK53_RS02005 0.88 -0.2 474,481 - LRK53_RS02005 0.88 -1.1 474,481 - LRK53_RS02005 0.88 -0.8 474,481 - LRK53_RS02005 0.88 -1.5 474,481 - LRK53_RS02005 0.88 -0.1 474,566 + +0.1 474,566 + -0.5 474,566 + -0.9 474,567 - +0.1 474,767 - -0.6 474,774 + -0.9 474,774 + -0.5 474,774 + -1.9 474,775 - -0.5 474,775 - -0.9 474,775 - +0.4 474,786 + -2.1 474,786 + +0.1 474,786 + +0.1 474,786 + -1.2 474,806 + +0.3 474,806 + -2.4 474,807 - -1.5 474,807 - +0.1 474,870 + -1.0 474,922 + +1.7 474,922 + -0.5 474,923 - -2.1 474,923 - +0.1 474,923 - -1.1 474,923 - -0.9 474,923 - +1.1 474,923 - -0.5 474,923 - -0.9 475,028 + +0.1 475,034 + -0.5 475,035 - -0.7 475,035 - -1.2 475,035 - -2.1 475,060 + +1.1 475,061 - -1.9 475,098 - +0.5 475,116 + -0.6 475,116 + +0.1 475,117 - -0.2 475,117 - +0.1 475,117 - -0.9 475,117 - +0.1 475,125 + -1.0 475,159 + LRK53_RS02010 0.14 -0.5 475,160 - LRK53_RS02010 0.14 +1.1 475,160 - LRK53_RS02010 0.14 +0.1 475,160 - LRK53_RS02010 0.14 -0.5 475,179 - LRK53_RS02010 0.18 -1.7 475,179 - LRK53_RS02010 0.18 -1.8 475,179 - LRK53_RS02010 0.18 -1.9 475,378 + LRK53_RS02010 0.59 -2.0 475,378 + LRK53_RS02010 0.59 -1.6 475,378 + LRK53_RS02010 0.59 -1.8 475,378 + LRK53_RS02010 0.59 -0.2 475,379 - LRK53_RS02010 0.59 -1.7 475,379 - LRK53_RS02010 0.59 -0.9 475,379 - LRK53_RS02010 0.59 +1.3 475,379 - LRK53_RS02010 0.59 -2.9 475,379 - LRK53_RS02010 0.59 +1.1 475,441 + LRK53_RS02010 0.72 -1.4 475,441 + LRK53_RS02010 0.72 +1.1 475,442 - LRK53_RS02010 0.72 -1.1 475,442 - LRK53_RS02010 0.72 -2.4 475,442 - LRK53_RS02010 0.72 +0.5 475,515 - LRK53_RS02010 0.87 +0.1 475,573 + -2.5 475,573 + -1.2 475,573 + -0.2 475,573 + -0.7 475,573 + -0.7 475,573 + +0.1 475,573 + -0.9 475,573 - -1.6 475,574 - +1.1 475,574 - -0.5 475,695 + -0.5 475,696 - -0.2 475,696 - -0.9 475,696 - -0.0 475,797 + -1.6 475,797 + -1.0 475,797 + -1.5 475,797 + +0.5 475,798 - -1.2 475,798 - -0.2 475,798 - -0.9 475,798 - -1.5 475,798 - -0.5 475,806 + -1.5 475,806 + -0.4 475,806 + +0.1 475,806 + -1.3 475,806 + +0.7 475,806 + -1.2 475,806 + +2.1 475,806 + +0.5 475,806 + -2.3 475,806 + +1.1 475,806 + -2.1 475,806 + +0.4 475,807 - -1.2 475,807 - -0.5 475,807 - -1.1 475,807 - -0.5 475,807 - -0.7 475,823 + -1.2 475,823 + -1.2 475,823 + +1.1 475,824 - +0.1 475,824 - -0.4 475,824 - -0.7 475,976 + LRK53_RS02015 0.21 -0.9 475,976 + LRK53_RS02015 0.21 -1.6 475,976 + LRK53_RS02015 0.21 +2.1 475,976 + LRK53_RS02015 0.21 -2.4 475,977 - LRK53_RS02015 0.21 -0.6 475,977 - LRK53_RS02015 0.21 -1.0 475,977 - LRK53_RS02015 0.21 +1.1 475,978 + LRK53_RS02015 0.21 +1.1 475,978 + LRK53_RS02015 0.21 +0.8 475,978 + LRK53_RS02015 0.21 -2.1 475,978 + LRK53_RS02015 0.21 +0.3 475,979 - LRK53_RS02015 0.21 -0.9 475,979 - LRK53_RS02015 0.21 -0.2 475,979 - LRK53_RS02015 0.21 -0.5 475,979 - LRK53_RS02015 0.21 +1.1 475,979 - LRK53_RS02015 0.21 -0.8 475,979 - LRK53_RS02015 0.21 -0.2 475,979 - LRK53_RS02015 0.21 -1.1 476,009 + LRK53_RS02015 0.26 -1.2 476,010 - LRK53_RS02015 0.26 +0.1 476,010 - LRK53_RS02015 0.26 -0.5 476,052 + LRK53_RS02015 0.33 +0.5 476,052 + LRK53_RS02015 0.33 +0.4 476,052 + LRK53_RS02015 0.33 +1.1 476,052 + LRK53_RS02015 0.33 -0.8 476,052 + LRK53_RS02015 0.33 -2.1 476,052 + LRK53_RS02015 0.33 -1.2 476,052 + LRK53_RS02015 0.33 -0.2 476,053 - LRK53_RS02015 0.33 -0.5 476,053 - LRK53_RS02015 0.33 -0.2 476,053 - LRK53_RS02015 0.33 +0.5 476,053 - LRK53_RS02015 0.33 -2.7 476,053 - LRK53_RS02015 0.33 -1.1 476,053 - LRK53_RS02015 0.33 +0.1 476,053 - LRK53_RS02015 0.33 +0.2 476,112 + LRK53_RS02015 0.42 +0.5 476,112 + LRK53_RS02015 0.42 -0.5 476,112 + LRK53_RS02015 0.42 -0.1 476,112 + LRK53_RS02015 0.42 +1.7 476,113 - LRK53_RS02015 0.42 -0.5 476,134 + LRK53_RS02015 0.46 -0.3 476,134 + LRK53_RS02015 0.46 -0.7 476,134 + LRK53_RS02015 0.46 +1.7 476,135 - LRK53_RS02015 0.46 -0.5 476,204 - LRK53_RS02015 0.57 -0.5 476,204 - LRK53_RS02015 0.57 -1.9 476,204 - LRK53_RS02015 0.57 -0.2 476,230 - LRK53_RS02015 0.61 +0.5 476,309 + LRK53_RS02015 0.73 -3.2 476,310 - LRK53_RS02015 0.73 -1.5 476,310 - LRK53_RS02015 0.73 -1.5 476,310 - LRK53_RS02015 0.73 -0.7 476,461 + +0.1 476,461 + -0.9 476,462 - -0.1 476,462 - -0.3 476,462 - +0.5 476,462 - +1.1 476,462 - -1.9 476,462 - -0.5 476,480 + +2.1 476,532 + -1.2 476,541 + -0.5 476,541 + -1.2 476,541 + -1.5 476,541 + -1.6 476,541 + -0.8 476,541 + -0.8 476,542 - +1.1 476,542 - -1.1
Or see this region's nucleotide sequence