Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS01345 and LRK53_RS01350 are separated by 134 nucleotides LRK53_RS01350 and LRK53_RS01355 are separated by 145 nucleotides LRK53_RS01355 and LRK53_RS01360 overlap by 23 nucleotides LRK53_RS01360 and LRK53_RS01365 are separated by 190 nucleotides LRK53_RS01365 and LRK53_RS01370 are separated by 67 nucleotides
LRK53_RS01345: LRK53_RS01345 - DNA primase, at 329,918 to 331,711
_RS01345
LRK53_RS01350: LRK53_RS01350 - DUF1328 domain-containing protein, at 331,846 to 332,016
_RS01350
LRK53_RS01355: LRK53_RS01355 - hypothetical protein, at 332,162 to 332,476
_RS01355
LRK53_RS01360: LRK53_RS01360 - GatB/YqeY domain-containing protein, at 332,454 to 332,900
_RS01360
LRK53_RS01365: LRK53_RS01365 - 30S ribosomal protein S21, at 333,091 to 333,306
_RS01365
LRK53_RS01370: LRK53_RS01370 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 333,374 to 334,423
_RS01370
Position (kb) 332
333 Strain fitness (log2 ratio) -2
-1
0
1
2 at 331.711 kb on + strand at 331.711 kb on + strand at 331.711 kb on + strand at 331.711 kb on + strand at 331.711 kb on + strand at 331.712 kb on - strand at 331.712 kb on - strand at 331.712 kb on - strand at 331.720 kb on + strand at 331.720 kb on + strand at 331.720 kb on + strand at 331.725 kb on + strand at 331.725 kb on + strand at 331.847 kb on + strand at 331.847 kb on + strand at 331.847 kb on + strand at 331.847 kb on + strand at 331.848 kb on - strand at 331.848 kb on - strand at 331.848 kb on - strand at 331.848 kb on - strand at 331.848 kb on - strand at 331.848 kb on - strand at 331.848 kb on - strand at 332.073 kb on + strand at 332.073 kb on + strand at 332.265 kb on + strand, within LRK53_RS01355 at 332.265 kb on + strand, within LRK53_RS01355 at 332.266 kb on - strand, within LRK53_RS01355 at 332.339 kb on + strand, within LRK53_RS01355 at 332.339 kb on + strand, within LRK53_RS01355 at 332.339 kb on + strand, within LRK53_RS01355 at 332.340 kb on - strand, within LRK53_RS01355 at 332.340 kb on - strand, within LRK53_RS01355 at 332.340 kb on - strand, within LRK53_RS01355 at 332.340 kb on - strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.389 kb on + strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.390 kb on - strand, within LRK53_RS01355 at 332.635 kb on + strand, within LRK53_RS01360 at 332.636 kb on - strand, within LRK53_RS01360 at 332.701 kb on + strand, within LRK53_RS01360 at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.925 kb on + strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.926 kb on - strand at 332.934 kb on + strand at 332.935 kb on - strand at 333.095 kb on + strand at 333.095 kb on + strand at 333.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 331,711 + -0.6 331,711 + -1.9 331,711 + -1.0 331,711 + +0.5 331,711 + -0.5 331,712 - -0.2 331,712 - -1.7 331,712 - -0.5 331,720 + -1.2 331,720 + -0.7 331,720 + -0.4 331,725 + +0.1 331,725 + +0.1 331,847 + -1.0 331,847 + -0.9 331,847 + -0.5 331,847 + -1.2 331,848 - +0.1 331,848 - +1.1 331,848 - -0.9 331,848 - +1.1 331,848 - -0.9 331,848 - +0.1 331,848 - +0.1 332,073 + +1.1 332,073 + -1.2 332,265 + LRK53_RS01355 0.33 -0.5 332,265 + LRK53_RS01355 0.33 -1.4 332,266 - LRK53_RS01355 0.33 -0.7 332,339 + LRK53_RS01355 0.56 -0.9 332,339 + LRK53_RS01355 0.56 -1.7 332,339 + LRK53_RS01355 0.56 -2.2 332,340 - LRK53_RS01355 0.57 +0.4 332,340 - LRK53_RS01355 0.57 +0.1 332,340 - LRK53_RS01355 0.57 -1.2 332,340 - LRK53_RS01355 0.57 -0.7 332,389 + LRK53_RS01355 0.72 -1.7 332,389 + LRK53_RS01355 0.72 -1.1 332,389 + LRK53_RS01355 0.72 -1.7 332,389 + LRK53_RS01355 0.72 -0.5 332,389 + LRK53_RS01355 0.72 -0.5 332,389 + LRK53_RS01355 0.72 +0.1 332,389 + LRK53_RS01355 0.72 -0.5 332,389 + LRK53_RS01355 0.72 +2.1 332,389 + LRK53_RS01355 0.72 -1.9 332,390 - LRK53_RS01355 0.72 -2.0 332,390 - LRK53_RS01355 0.72 -0.5 332,390 - LRK53_RS01355 0.72 -0.6 332,390 - LRK53_RS01355 0.72 -0.1 332,390 - LRK53_RS01355 0.72 -0.7 332,390 - LRK53_RS01355 0.72 +0.5 332,390 - LRK53_RS01355 0.72 -1.5 332,390 - LRK53_RS01355 0.72 -0.5 332,390 - LRK53_RS01355 0.72 +0.1 332,390 - LRK53_RS01355 0.72 -1.5 332,390 - LRK53_RS01355 0.72 +0.1 332,390 - LRK53_RS01355 0.72 +1.1 332,635 + LRK53_RS01360 0.40 -0.3 332,636 - LRK53_RS01360 0.41 -0.5 332,701 + LRK53_RS01360 0.55 +0.1 332,925 + +1.1 332,925 + +0.1 332,925 + -1.9 332,925 + -1.2 332,925 + +1.7 332,925 + -0.8 332,925 + -0.5 332,925 + -0.5 332,925 + -0.2 332,925 + -1.2 332,925 + -0.5 332,925 + +1.1 332,925 + -0.5 332,925 + +0.3 332,926 - +0.4 332,926 - -1.2 332,926 - +0.7 332,926 - -0.4 332,926 - -0.1 332,926 - -1.2 332,926 - -0.3 332,926 - -1.0 332,926 - -1.6 332,926 - -1.9 332,926 - -0.9 332,926 - +0.5 332,926 - +0.1 332,926 - +0.1 332,926 - -1.3 332,926 - -1.0 332,926 - +1.1 332,926 - -1.4 332,926 - -0.9 332,926 - -1.1 332,926 - -0.8 332,926 - -1.1 332,926 - -0.9 332,926 - -0.8 332,926 - -0.2 332,926 - -1.0 332,934 + +2.1 332,935 - -0.7 333,095 + +0.1 333,095 + +0.9 333,096 - -0.8
Or see this region's nucleotide sequence