Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00615 and LRK53_RS00620 are separated by 30 nucleotides LRK53_RS00620 and LRK53_RS00625 are separated by 167 nucleotides
LRK53_RS00615: LRK53_RS00615 - histidine kinase, at 138,280 to 139,332
_RS00615
LRK53_RS00620: LRK53_RS00620 - alpha/beta hydrolase fold domain-containing protein, at 139,363 to 140,025
_RS00620
LRK53_RS00625: LRK53_RS00625 - prolyl oligopeptidase family serine peptidase, at 140,193 to 142,355
_RS00625
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 138.368 kb on + strand at 138.650 kb on + strand, within LRK53_RS00615 at 138.650 kb on + strand, within LRK53_RS00615 at 138.650 kb on + strand, within LRK53_RS00615 at 138.650 kb on + strand, within LRK53_RS00615 at 138.651 kb on - strand, within LRK53_RS00615 at 138.651 kb on - strand, within LRK53_RS00615 at 138.826 kb on - strand, within LRK53_RS00615 at 138.874 kb on + strand, within LRK53_RS00615 at 138.874 kb on + strand, within LRK53_RS00615 at 138.874 kb on + strand, within LRK53_RS00615 at 138.875 kb on - strand, within LRK53_RS00615 at 138.875 kb on - strand, within LRK53_RS00615 at 138.875 kb on - strand, within LRK53_RS00615 at 138.896 kb on + strand, within LRK53_RS00615 at 138.896 kb on + strand, within LRK53_RS00615 at 138.896 kb on + strand, within LRK53_RS00615 at 138.896 kb on + strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.897 kb on - strand, within LRK53_RS00615 at 138.902 kb on + strand, within LRK53_RS00615 at 138.902 kb on + strand, within LRK53_RS00615 at 138.902 kb on + strand, within LRK53_RS00615 at 138.903 kb on - strand, within LRK53_RS00615 at 138.903 kb on - strand, within LRK53_RS00615 at 138.903 kb on - strand, within LRK53_RS00615 at 138.903 kb on - strand, within LRK53_RS00615 at 139.068 kb on - strand, within LRK53_RS00615 at 139.169 kb on + strand, within LRK53_RS00615 at 139.169 kb on + strand, within LRK53_RS00615 at 139.169 kb on + strand, within LRK53_RS00615 at 139.170 kb on - strand, within LRK53_RS00615 at 139.170 kb on - strand, within LRK53_RS00615 at 139.170 kb on - strand, within LRK53_RS00615 at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.342 kb on + strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.343 kb on - strand at 139.364 kb on + strand at 139.364 kb on + strand at 139.364 kb on + strand at 139.365 kb on - strand at 139.365 kb on - strand at 139.365 kb on - strand at 139.365 kb on - strand at 139.436 kb on + strand, within LRK53_RS00620 at 139.436 kb on + strand, within LRK53_RS00620 at 139.436 kb on + strand, within LRK53_RS00620 at 139.437 kb on - strand, within LRK53_RS00620 at 139.437 kb on - strand, within LRK53_RS00620 at 139.437 kb on - strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.601 kb on + strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.602 kb on - strand, within LRK53_RS00620 at 139.817 kb on + strand, within LRK53_RS00620 at 139.817 kb on + strand, within LRK53_RS00620 at 139.817 kb on + strand, within LRK53_RS00620 at 139.817 kb on + strand, within LRK53_RS00620 at 139.818 kb on - strand, within LRK53_RS00620 at 139.818 kb on - strand, within LRK53_RS00620 at 139.818 kb on - strand, within LRK53_RS00620 at 139.818 kb on - strand, within LRK53_RS00620 at 139.980 kb on - strand at 139.980 kb on - strand at 140.060 kb on + strand at 140.060 kb on + strand at 140.060 kb on + strand at 140.061 kb on - strand at 140.061 kb on - strand at 140.089 kb on - strand at 140.089 kb on - strand at 140.089 kb on - strand at 140.089 kb on - strand at 140.176 kb on + strand at 140.176 kb on + strand at 140.176 kb on + strand at 140.177 kb on - strand at 140.177 kb on - strand at 140.177 kb on - strand at 140.177 kb on - strand at 140.177 kb on - strand at 140.194 kb on + strand at 140.194 kb on + strand at 140.195 kb on - strand at 140.426 kb on - strand, within LRK53_RS00625 at 140.462 kb on - strand, within LRK53_RS00625 at 140.462 kb on - strand, within LRK53_RS00625 at 140.462 kb on - strand, within LRK53_RS00625 at 140.521 kb on + strand, within LRK53_RS00625 at 140.521 kb on + strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.522 kb on - strand, within LRK53_RS00625 at 140.563 kb on + strand, within LRK53_RS00625 at 140.563 kb on + strand, within LRK53_RS00625 at 140.563 kb on + strand, within LRK53_RS00625 at 140.563 kb on + strand, within LRK53_RS00625 at 140.563 kb on + strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.564 kb on - strand, within LRK53_RS00625 at 140.803 kb on + strand, within LRK53_RS00625 at 140.804 kb on - strand, within LRK53_RS00625 at 140.884 kb on + strand, within LRK53_RS00625 at 140.884 kb on + strand, within LRK53_RS00625 at 140.885 kb on - strand, within LRK53_RS00625 at 140.885 kb on - strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.890 kb on + strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625 at 140.891 kb on - strand, within LRK53_RS00625
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 138,368 + -1.1 138,650 + LRK53_RS00615 0.35 -0.6 138,650 + LRK53_RS00615 0.35 +0.3 138,650 + LRK53_RS00615 0.35 -1.4 138,650 + LRK53_RS00615 0.35 -2.2 138,651 - LRK53_RS00615 0.35 -1.1 138,651 - LRK53_RS00615 0.35 -1.4 138,826 - LRK53_RS00615 0.52 -1.1 138,874 + LRK53_RS00615 0.56 -0.7 138,874 + LRK53_RS00615 0.56 -0.4 138,874 + LRK53_RS00615 0.56 -2.1 138,875 - LRK53_RS00615 0.57 +0.2 138,875 - LRK53_RS00615 0.57 +0.2 138,875 - LRK53_RS00615 0.57 -1.8 138,896 + LRK53_RS00615 0.58 -1.1 138,896 + LRK53_RS00615 0.58 -0.8 138,896 + LRK53_RS00615 0.58 +0.2 138,896 + LRK53_RS00615 0.58 -0.8 138,897 - LRK53_RS00615 0.59 -0.7 138,897 - LRK53_RS00615 0.59 -0.8 138,897 - LRK53_RS00615 0.59 -1.4 138,897 - LRK53_RS00615 0.59 -0.1 138,897 - LRK53_RS00615 0.59 -1.5 138,897 - LRK53_RS00615 0.59 -0.1 138,902 + LRK53_RS00615 0.59 +0.2 138,902 + LRK53_RS00615 0.59 +0.8 138,902 + LRK53_RS00615 0.59 -0.4 138,903 - LRK53_RS00615 0.59 -1.1 138,903 - LRK53_RS00615 0.59 -0.4 138,903 - LRK53_RS00615 0.59 -2.3 138,903 - LRK53_RS00615 0.59 +0.6 139,068 - LRK53_RS00615 0.75 +1.8 139,169 + LRK53_RS00615 0.84 +1.2 139,169 + LRK53_RS00615 0.84 -1.2 139,169 + LRK53_RS00615 0.84 -0.7 139,170 - LRK53_RS00615 0.85 -0.8 139,170 - LRK53_RS00615 0.85 -0.5 139,170 - LRK53_RS00615 0.85 -0.7 139,342 + +1.5 139,342 + -0.8 139,342 + -1.1 139,342 + -1.4 139,342 + +0.2 139,342 + -0.6 139,342 + -0.4 139,342 + +0.6 139,342 + +0.1 139,343 - -0.8 139,343 - -1.1 139,343 - -0.0 139,343 - -0.5 139,343 - -0.5 139,343 - -1.2 139,343 - -0.1 139,343 - -1.4 139,343 - +0.2 139,343 - +0.8 139,343 - -1.1 139,343 - -0.2 139,364 + +0.6 139,364 + -1.2 139,364 + -0.4 139,365 - -0.4 139,365 - -1.1 139,365 - +1.2 139,365 - -0.6 139,436 + LRK53_RS00620 0.11 -1.1 139,436 + LRK53_RS00620 0.11 +1.2 139,436 + LRK53_RS00620 0.11 +0.2 139,437 - LRK53_RS00620 0.11 -1.6 139,437 - LRK53_RS00620 0.11 -1.4 139,437 - LRK53_RS00620 0.11 +0.2 139,601 + LRK53_RS00620 0.36 -0.8 139,601 + LRK53_RS00620 0.36 -2.4 139,601 + LRK53_RS00620 0.36 -2.1 139,601 + LRK53_RS00620 0.36 -1.9 139,601 + LRK53_RS00620 0.36 -3.0 139,601 + LRK53_RS00620 0.36 +1.2 139,601 + LRK53_RS00620 0.36 -1.4 139,601 + LRK53_RS00620 0.36 -0.7 139,601 + LRK53_RS00620 0.36 +1.2 139,602 - LRK53_RS00620 0.36 +0.2 139,602 - LRK53_RS00620 0.36 +0.2 139,602 - LRK53_RS00620 0.36 -1.6 139,602 - LRK53_RS00620 0.36 +1.2 139,602 - LRK53_RS00620 0.36 -2.4 139,602 - LRK53_RS00620 0.36 +2.2 139,602 - LRK53_RS00620 0.36 -0.9 139,602 - LRK53_RS00620 0.36 -0.4 139,602 - LRK53_RS00620 0.36 -0.8 139,602 - LRK53_RS00620 0.36 -2.4 139,602 - LRK53_RS00620 0.36 -0.6 139,817 + LRK53_RS00620 0.68 -1.4 139,817 + LRK53_RS00620 0.68 -0.8 139,817 + LRK53_RS00620 0.68 -1.1 139,817 + LRK53_RS00620 0.68 -1.0 139,818 - LRK53_RS00620 0.69 -2.4 139,818 - LRK53_RS00620 0.69 -2.3 139,818 - LRK53_RS00620 0.69 +0.2 139,818 - LRK53_RS00620 0.69 -1.8 139,980 - -0.1 139,980 - -0.4 140,060 + -0.2 140,060 + -0.4 140,060 + -0.4 140,061 - -0.6 140,061 - -1.3 140,089 - -0.0 140,089 - -0.5 140,089 - +0.9 140,089 - -0.4 140,176 + -1.8 140,176 + +1.2 140,176 + -1.7 140,177 - +1.2 140,177 - -0.4 140,177 - +1.2 140,177 - -0.2 140,177 - -0.6 140,194 + +0.6 140,194 + -2.2 140,195 - +0.5 140,426 - LRK53_RS00625 0.11 -3.0 140,462 - LRK53_RS00625 0.12 +0.7 140,462 - LRK53_RS00625 0.12 -0.8 140,462 - LRK53_RS00625 0.12 +0.5 140,521 + LRK53_RS00625 0.15 -0.0 140,521 + LRK53_RS00625 0.15 -0.8 140,522 - LRK53_RS00625 0.15 -1.1 140,522 - LRK53_RS00625 0.15 -0.6 140,522 - LRK53_RS00625 0.15 -1.3 140,522 - LRK53_RS00625 0.15 +2.2 140,522 - LRK53_RS00625 0.15 -0.1 140,522 - LRK53_RS00625 0.15 +2.2 140,563 + LRK53_RS00625 0.17 +0.2 140,563 + LRK53_RS00625 0.17 +0.5 140,563 + LRK53_RS00625 0.17 -0.8 140,563 + LRK53_RS00625 0.17 -1.4 140,563 + LRK53_RS00625 0.17 -1.1 140,564 - LRK53_RS00625 0.17 -2.0 140,564 - LRK53_RS00625 0.17 -1.1 140,564 - LRK53_RS00625 0.17 -0.7 140,564 - LRK53_RS00625 0.17 -0.4 140,564 - LRK53_RS00625 0.17 +0.6 140,564 - LRK53_RS00625 0.17 -0.1 140,803 + LRK53_RS00625 0.28 -0.5 140,804 - LRK53_RS00625 0.28 -0.4 140,884 + LRK53_RS00625 0.32 +1.2 140,884 + LRK53_RS00625 0.32 +2.2 140,885 - LRK53_RS00625 0.32 -1.3 140,885 - LRK53_RS00625 0.32 +1.2 140,890 + LRK53_RS00625 0.32 +1.2 140,890 + LRK53_RS00625 0.32 +0.2 140,890 + LRK53_RS00625 0.32 -2.1 140,890 + LRK53_RS00625 0.32 -0.4 140,890 + LRK53_RS00625 0.32 +0.4 140,890 + LRK53_RS00625 0.32 -0.8 140,890 + LRK53_RS00625 0.32 -0.5 140,890 + LRK53_RS00625 0.32 +0.2 140,891 - LRK53_RS00625 0.32 -0.4 140,891 - LRK53_RS00625 0.32 -1.3 140,891 - LRK53_RS00625 0.32 -1.3 140,891 - LRK53_RS00625 0.32 +0.2 140,891 - LRK53_RS00625 0.32 -0.1 140,891 - LRK53_RS00625 0.32 -1.1
Or see this region's nucleotide sequence