Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00305 and LRK53_RS00310 are separated by 23 nucleotides LRK53_RS00310 and LRK53_RS00315 are separated by 173 nucleotides LRK53_RS00315 and LRK53_RS00320 are separated by 189 nucleotides
LRK53_RS00305: LRK53_RS00305 - TorF family putative porin, at 70,344 to 71,105
_RS00305
LRK53_RS00310: LRK53_RS00310 - P-II family nitrogen regulator, at 71,129 to 71,467
_RS00310
LRK53_RS00315: LRK53_RS00315 - ammonium transporter, at 71,641 to 72,963
_RS00315
LRK53_RS00320: LRK53_RS00320 - type I glutamate--ammonia ligase, at 73,153 to 74,562
_RS00320
Position (kb) 71
72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 70.696 kb on - strand, within LRK53_RS00305 at 70.702 kb on - strand, within LRK53_RS00305 at 70.707 kb on + strand, within LRK53_RS00305 at 70.758 kb on + strand, within LRK53_RS00305 at 70.758 kb on + strand, within LRK53_RS00305 at 70.758 kb on + strand, within LRK53_RS00305 at 70.759 kb on - strand, within LRK53_RS00305 at 70.759 kb on - strand, within LRK53_RS00305 at 70.759 kb on - strand, within LRK53_RS00305 at 70.794 kb on + strand, within LRK53_RS00305 at 70.794 kb on + strand, within LRK53_RS00305 at 70.794 kb on + strand, within LRK53_RS00305 at 70.794 kb on + strand, within LRK53_RS00305 at 70.794 kb on + strand, within LRK53_RS00305 at 70.795 kb on - strand, within LRK53_RS00305 at 70.795 kb on - strand, within LRK53_RS00305 at 70.800 kb on + strand, within LRK53_RS00305 at 70.800 kb on + strand, within LRK53_RS00305 at 70.800 kb on + strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.801 kb on - strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.921 kb on + strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 70.922 kb on - strand, within LRK53_RS00305 at 71.037 kb on + strand at 71.037 kb on + strand at 71.038 kb on - strand at 71.038 kb on - strand at 71.038 kb on - strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.112 kb on + strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.113 kb on - strand at 71.159 kb on + strand at 71.159 kb on + strand at 71.160 kb on - strand at 71.264 kb on + strand, within LRK53_RS00310 at 71.265 kb on - strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.279 kb on + strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.280 kb on - strand, within LRK53_RS00310 at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.527 kb on + strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.528 kb on - strand at 71.561 kb on + strand at 71.562 kb on - strand at 71.562 kb on - strand at 71.815 kb on + strand, within LRK53_RS00315 at 71.816 kb on - strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.828 kb on + strand, within LRK53_RS00315 at 71.829 kb on - strand, within LRK53_RS00315 at 71.829 kb on - strand, within LRK53_RS00315 at 71.829 kb on - strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.905 kb on + strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.906 kb on - strand, within LRK53_RS00315 at 71.912 kb on - strand, within LRK53_RS00315 at 72.031 kb on + strand, within LRK53_RS00315 at 72.031 kb on + strand, within LRK53_RS00315 at 72.031 kb on + strand, within LRK53_RS00315 at 72.031 kb on + strand, within LRK53_RS00315 at 72.031 kb on + strand, within LRK53_RS00315 at 72.032 kb on - strand, within LRK53_RS00315 at 72.032 kb on - strand, within LRK53_RS00315 at 72.032 kb on - strand, within LRK53_RS00315 at 72.157 kb on + strand, within LRK53_RS00315 at 72.157 kb on + strand, within LRK53_RS00315 at 72.157 kb on + strand, within LRK53_RS00315 at 72.157 kb on + strand, within LRK53_RS00315 at 72.158 kb on - strand, within LRK53_RS00315 at 72.778 kb on + strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.779 kb on - strand, within LRK53_RS00315 at 72.823 kb on - strand, within LRK53_RS00315 at 72.823 kb on - strand, within LRK53_RS00315 at 72.862 kb on + strand at 72.863 kb on - strand at 72.897 kb on - strand at 72.897 kb on - strand at 72.901 kb on + strand at 72.901 kb on + strand at 72.902 kb on - strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.930 kb on + strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.931 kb on - strand at 72.952 kb on + strand at 72.952 kb on + strand at 72.952 kb on + strand at 72.952 kb on + strand at 72.953 kb on - strand at 73.008 kb on + strand at 73.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 70,696 - LRK53_RS00305 0.46 -0.1 70,702 - LRK53_RS00305 0.47 +1.2 70,707 + LRK53_RS00305 0.48 -1.2 70,758 + LRK53_RS00305 0.54 -0.1 70,758 + LRK53_RS00305 0.54 -0.8 70,758 + LRK53_RS00305 0.54 +1.2 70,759 - LRK53_RS00305 0.54 -0.8 70,759 - LRK53_RS00305 0.54 -1.4 70,759 - LRK53_RS00305 0.54 -0.6 70,794 + LRK53_RS00305 0.59 -0.9 70,794 + LRK53_RS00305 0.59 +1.2 70,794 + LRK53_RS00305 0.59 +0.2 70,794 + LRK53_RS00305 0.59 -0.8 70,794 + LRK53_RS00305 0.59 -1.1 70,795 - LRK53_RS00305 0.59 -0.9 70,795 - LRK53_RS00305 0.59 +0.8 70,800 + LRK53_RS00305 0.60 -0.4 70,800 + LRK53_RS00305 0.60 -1.1 70,800 + LRK53_RS00305 0.60 -0.1 70,801 - LRK53_RS00305 0.60 -0.4 70,801 - LRK53_RS00305 0.60 -0.9 70,801 - LRK53_RS00305 0.60 -1.5 70,801 - LRK53_RS00305 0.60 +0.2 70,801 - LRK53_RS00305 0.60 -0.9 70,801 - LRK53_RS00305 0.60 -0.4 70,921 + LRK53_RS00305 0.76 +0.4 70,921 + LRK53_RS00305 0.76 +0.4 70,921 + LRK53_RS00305 0.76 -1.4 70,921 + LRK53_RS00305 0.76 -1.4 70,921 + LRK53_RS00305 0.76 -1.1 70,921 + LRK53_RS00305 0.76 -1.0 70,921 + LRK53_RS00305 0.76 -2.2 70,921 + LRK53_RS00305 0.76 +0.8 70,921 + LRK53_RS00305 0.76 +0.6 70,921 + LRK53_RS00305 0.76 +1.2 70,921 + LRK53_RS00305 0.76 +0.6 70,921 + LRK53_RS00305 0.76 -0.8 70,922 - LRK53_RS00305 0.76 -0.8 70,922 - LRK53_RS00305 0.76 +0.2 70,922 - LRK53_RS00305 0.76 -0.8 70,922 - LRK53_RS00305 0.76 +0.5 70,922 - LRK53_RS00305 0.76 -1.4 70,922 - LRK53_RS00305 0.76 -1.1 70,922 - LRK53_RS00305 0.76 +2.2 70,922 - LRK53_RS00305 0.76 +0.8 71,037 + -0.4 71,037 + -0.4 71,038 - +1.2 71,038 - -0.5 71,038 - +0.2 71,059 - -0.9 71,059 - +0.2 71,059 - -1.2 71,112 + -0.8 71,112 + -1.5 71,112 + +0.2 71,112 + -0.4 71,112 + -1.1 71,112 + -0.8 71,112 + -1.2 71,112 + +0.2 71,112 + -0.8 71,112 + +0.1 71,112 + +1.2 71,112 + -1.1 71,112 + +0.9 71,112 + -0.4 71,112 + -0.4 71,112 + +0.2 71,112 + -1.8 71,112 + -0.9 71,112 + +0.5 71,112 + +0.2 71,113 - -2.1 71,113 - +2.2 71,113 - +0.2 71,113 - -0.8 71,113 - -2.4 71,113 - -1.2 71,113 - -0.8 71,113 - -3.1 71,113 - -0.0 71,113 - +0.8 71,113 - +2.2 71,113 - -0.0 71,113 - +2.2 71,113 - -0.0 71,113 - -0.4 71,113 - -0.4 71,113 - -1.5 71,113 - +0.2 71,113 - +1.2 71,113 - -1.4 71,113 - +0.2 71,113 - -1.1 71,113 - +0.6 71,113 - +0.6 71,113 - -1.6 71,113 - +1.2 71,113 - -0.8 71,113 - +0.1 71,159 + -1.6 71,159 + +0.6 71,160 - +0.2 71,264 + LRK53_RS00310 0.40 -1.7 71,265 - LRK53_RS00310 0.40 +0.5 71,279 + LRK53_RS00310 0.44 -0.8 71,279 + LRK53_RS00310 0.44 -1.6 71,279 + LRK53_RS00310 0.44 -0.1 71,279 + LRK53_RS00310 0.44 -0.8 71,279 + LRK53_RS00310 0.44 +2.2 71,279 + LRK53_RS00310 0.44 -0.8 71,279 + LRK53_RS00310 0.44 -0.4 71,279 + LRK53_RS00310 0.44 +0.2 71,279 + LRK53_RS00310 0.44 +0.2 71,279 + LRK53_RS00310 0.44 +2.8 71,279 + LRK53_RS00310 0.44 +0.7 71,279 + LRK53_RS00310 0.44 +0.6 71,279 + LRK53_RS00310 0.44 -2.2 71,279 + LRK53_RS00310 0.44 +0.2 71,280 - LRK53_RS00310 0.45 +0.2 71,280 - LRK53_RS00310 0.45 +0.2 71,280 - LRK53_RS00310 0.45 +0.2 71,280 - LRK53_RS00310 0.45 -0.4 71,280 - LRK53_RS00310 0.45 -0.4 71,280 - LRK53_RS00310 0.45 -1.1 71,280 - LRK53_RS00310 0.45 -2.0 71,527 + +0.2 71,527 + -0.0 71,527 + -0.7 71,527 + -1.7 71,527 + +0.8 71,527 + -1.4 71,527 + -1.7 71,527 + -0.0 71,527 + -0.4 71,527 + +1.2 71,527 + -1.1 71,527 + -0.8 71,527 + -1.6 71,527 + +0.2 71,527 + -1.5 71,527 + -1.0 71,527 + -0.9 71,528 - -2.0 71,528 - -0.8 71,528 - -0.4 71,528 - -0.9 71,528 - -0.9 71,528 - +0.8 71,528 - -0.1 71,528 - -0.8 71,528 - +0.0 71,528 - +0.4 71,528 - -0.5 71,528 - -0.9 71,528 - -2.4 71,528 - +0.2 71,528 - +1.8 71,528 - +0.2 71,528 - -1.4 71,528 - -0.1 71,528 - +0.2 71,528 - -1.6 71,528 - -0.6 71,528 - -1.4 71,561 + -1.4 71,562 - +0.8 71,562 - -1.4 71,815 + LRK53_RS00315 0.13 -0.8 71,816 - LRK53_RS00315 0.13 -1.6 71,828 + LRK53_RS00315 0.14 -1.6 71,828 + LRK53_RS00315 0.14 +0.6 71,828 + LRK53_RS00315 0.14 -0.9 71,828 + LRK53_RS00315 0.14 -0.1 71,828 + LRK53_RS00315 0.14 -1.4 71,828 + LRK53_RS00315 0.14 -1.1 71,828 + LRK53_RS00315 0.14 +0.5 71,828 + LRK53_RS00315 0.14 +0.8 71,828 + LRK53_RS00315 0.14 +1.2 71,829 - LRK53_RS00315 0.14 -0.4 71,829 - LRK53_RS00315 0.14 -0.4 71,829 - LRK53_RS00315 0.14 +0.2 71,905 + LRK53_RS00315 0.20 -2.3 71,905 + LRK53_RS00315 0.20 -0.5 71,905 + LRK53_RS00315 0.20 -1.4 71,905 + LRK53_RS00315 0.20 +0.0 71,905 + LRK53_RS00315 0.20 +0.4 71,905 + LRK53_RS00315 0.20 -1.4 71,905 + LRK53_RS00315 0.20 -1.8 71,905 + LRK53_RS00315 0.20 -0.8 71,905 + LRK53_RS00315 0.20 -0.4 71,905 + LRK53_RS00315 0.20 +2.2 71,905 + LRK53_RS00315 0.20 -1.4 71,906 - LRK53_RS00315 0.20 -0.7 71,906 - LRK53_RS00315 0.20 -0.2 71,906 - LRK53_RS00315 0.20 -0.8 71,906 - LRK53_RS00315 0.20 +0.2 71,906 - LRK53_RS00315 0.20 +0.2 71,906 - LRK53_RS00315 0.20 -0.8 71,906 - LRK53_RS00315 0.20 +1.2 71,906 - LRK53_RS00315 0.20 +0.1 71,906 - LRK53_RS00315 0.20 +0.2 71,912 - LRK53_RS00315 0.20 +1.2 72,031 + LRK53_RS00315 0.29 -0.5 72,031 + LRK53_RS00315 0.29 +0.6 72,031 + LRK53_RS00315 0.29 -0.7 72,031 + LRK53_RS00315 0.29 -0.4 72,031 + LRK53_RS00315 0.29 -0.6 72,032 - LRK53_RS00315 0.30 -0.6 72,032 - LRK53_RS00315 0.30 -1.6 72,032 - LRK53_RS00315 0.30 +0.5 72,157 + LRK53_RS00315 0.39 +0.2 72,157 + LRK53_RS00315 0.39 +0.2 72,157 + LRK53_RS00315 0.39 +1.2 72,157 + LRK53_RS00315 0.39 +1.2 72,158 - LRK53_RS00315 0.39 +0.6 72,778 + LRK53_RS00315 0.86 -1.0 72,779 - LRK53_RS00315 0.86 -1.4 72,779 - LRK53_RS00315 0.86 -0.2 72,779 - LRK53_RS00315 0.86 +1.1 72,779 - LRK53_RS00315 0.86 +2.2 72,779 - LRK53_RS00315 0.86 -1.1 72,779 - LRK53_RS00315 0.86 +0.5 72,779 - LRK53_RS00315 0.86 +0.7 72,823 - LRK53_RS00315 0.89 -0.2 72,823 - LRK53_RS00315 0.89 -0.8 72,862 + +2.8 72,863 - -1.7 72,897 - +1.2 72,897 - -1.7 72,901 + +0.2 72,901 + -0.8 72,902 - +1.8 72,930 + -0.7 72,930 + +1.2 72,930 + -0.1 72,930 + -0.3 72,930 + -1.4 72,930 + -1.4 72,930 + -1.0 72,930 + -0.8 72,931 - -0.4 72,931 - -0.8 72,931 - +2.2 72,931 - +1.8 72,931 - -2.4 72,931 - +0.8 72,931 - -0.7 72,931 - -0.9 72,931 - -2.4 72,931 - -1.8 72,931 - -0.8 72,931 - -1.2 72,931 - -0.9 72,931 - +0.8 72,952 + -2.9 72,952 + -1.1 72,952 + -0.2 72,952 + -1.8 72,953 - +0.2 73,008 + -0.8 73,008 + -2.0
Or see this region's nucleotide sequence