Experiment: K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt norR and gutQ overlap by 4 nucleotides gutQ and srlR overlap by 8 nucleotides srlR and gutM are separated by 66 nucleotides
JDDGAC_24315: norR - nitric oxide reductase transcriptional regulator NorR, at 4,664,420 to 4,665,934
norR
JDDGAC_24320: gutQ - arabinose-5-phosphate isomerase GutQ, at 4,665,931 to 4,666,896
gutQ
JDDGAC_24325: srlR - glucitol operon DNA-binding transcriptional repressor SrlR, at 4,666,889 to 4,667,662
srlR
JDDGAC_24330: gutM - transcriptional regulator GutM, at 4,667,729 to 4,668,088
gutM
Position (kb)
4665
4666
4667 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4665.364 kb on + strand, within norR at 4665.579 kb on + strand, within norR at 4665.579 kb on + strand, within norR at 4665.580 kb on - strand, within norR at 4665.612 kb on - strand, within norR at 4665.649 kb on + strand, within norR at 4665.820 kb on + strand at 4665.821 kb on - strand at 4665.932 kb on + strand at 4665.933 kb on - strand at 4665.936 kb on + strand at 4665.950 kb on + strand at 4666.171 kb on + strand, within gutQ at 4666.171 kb on + strand, within gutQ at 4666.172 kb on - strand, within gutQ at 4666.192 kb on + strand, within gutQ at 4666.192 kb on + strand, within gutQ at 4666.192 kb on + strand, within gutQ at 4666.233 kb on + strand, within gutQ at 4666.234 kb on - strand, within gutQ at 4666.234 kb on - strand, within gutQ at 4666.365 kb on + strand, within gutQ at 4666.479 kb on + strand, within gutQ at 4666.604 kb on + strand, within gutQ at 4666.611 kb on + strand, within gutQ at 4666.611 kb on + strand, within gutQ at 4666.858 kb on + strand at 4666.910 kb on + strand at 4666.963 kb on + strand at 4667.010 kb on - strand, within srlR at 4667.010 kb on - strand, within srlR at 4667.097 kb on + strand, within srlR at 4667.098 kb on - strand, within srlR at 4667.098 kb on - strand, within srlR at 4667.098 kb on - strand, within srlR at 4667.098 kb on - strand, within srlR at 4667.252 kb on - strand, within srlR at 4667.252 kb on - strand, within srlR at 4667.320 kb on + strand, within srlR at 4667.323 kb on + strand, within srlR at 4667.324 kb on - strand, within srlR at 4667.360 kb on - strand, within srlR at 4667.398 kb on + strand, within srlR at 4667.398 kb on + strand, within srlR at 4667.455 kb on + strand, within srlR at 4667.456 kb on - strand, within srlR at 4667.464 kb on + strand, within srlR at 4667.464 kb on + strand, within srlR at 4667.506 kb on - strand, within srlR at 4667.509 kb on + strand, within srlR at 4667.509 kb on + strand, within srlR at 4667.509 kb on + strand, within srlR at 4667.510 kb on - strand, within srlR at 4667.510 kb on - strand, within srlR at 4667.510 kb on - strand, within srlR at 4667.558 kb on - strand, within srlR at 4667.558 kb on - strand, within srlR at 4667.625 kb on + strand at 4667.625 kb on + strand at 4667.626 kb on - strand at 4667.673 kb on - strand at 4667.682 kb on - strand at 4667.731 kb on - strand at 4667.824 kb on + strand, within gutM at 4667.878 kb on + strand, within gutM
Per-strain Table
Position Strand Gene LocusTag Fraction K30 remove 4,665,364 + norR JDDGAC_24315 0.62 -0.5 4,665,579 + norR JDDGAC_24315 0.77 +0.3 4,665,579 + norR JDDGAC_24315 0.77 +0.1 4,665,580 - norR JDDGAC_24315 0.77 -0.9 4,665,612 - norR JDDGAC_24315 0.79 -0.9 4,665,649 + norR JDDGAC_24315 0.81 -0.0 4,665,820 + +1.3 4,665,821 - +1.0 4,665,932 + -1.4 4,665,933 - -1.3 4,665,936 + -0.6 4,665,950 + -1.0 4,666,171 + gutQ JDDGAC_24320 0.25 -0.9 4,666,171 + gutQ JDDGAC_24320 0.25 +0.0 4,666,172 - gutQ JDDGAC_24320 0.25 -1.1 4,666,192 + gutQ JDDGAC_24320 0.27 -1.6 4,666,192 + gutQ JDDGAC_24320 0.27 -1.6 4,666,192 + gutQ JDDGAC_24320 0.27 -0.4 4,666,233 + gutQ JDDGAC_24320 0.31 +0.3 4,666,234 - gutQ JDDGAC_24320 0.31 -0.1 4,666,234 - gutQ JDDGAC_24320 0.31 -0.1 4,666,365 + gutQ JDDGAC_24320 0.45 -0.9 4,666,479 + gutQ JDDGAC_24320 0.57 -0.5 4,666,604 + gutQ JDDGAC_24320 0.70 -0.9 4,666,611 + gutQ JDDGAC_24320 0.70 +0.8 4,666,611 + gutQ JDDGAC_24320 0.70 -2.1 4,666,858 + +0.9 4,666,910 + -0.5 4,666,963 + -0.2 4,667,010 - srlR JDDGAC_24325 0.16 -0.0 4,667,010 - srlR JDDGAC_24325 0.16 -1.4 4,667,097 + srlR JDDGAC_24325 0.27 +0.7 4,667,098 - srlR JDDGAC_24325 0.27 +0.8 4,667,098 - srlR JDDGAC_24325 0.27 -0.1 4,667,098 - srlR JDDGAC_24325 0.27 -0.9 4,667,098 - srlR JDDGAC_24325 0.27 +1.3 4,667,252 - srlR JDDGAC_24325 0.47 +0.6 4,667,252 - srlR JDDGAC_24325 0.47 -0.7 4,667,320 + srlR JDDGAC_24325 0.56 -0.1 4,667,323 + srlR JDDGAC_24325 0.56 +1.8 4,667,324 - srlR JDDGAC_24325 0.56 -0.1 4,667,360 - srlR JDDGAC_24325 0.61 -0.5 4,667,398 + srlR JDDGAC_24325 0.66 +1.0 4,667,398 + srlR JDDGAC_24325 0.66 -1.0 4,667,455 + srlR JDDGAC_24325 0.73 +0.4 4,667,456 - srlR JDDGAC_24325 0.73 -0.0 4,667,464 + srlR JDDGAC_24325 0.74 -0.2 4,667,464 + srlR JDDGAC_24325 0.74 -0.2 4,667,506 - srlR JDDGAC_24325 0.80 -0.2 4,667,509 + srlR JDDGAC_24325 0.80 +0.4 4,667,509 + srlR JDDGAC_24325 0.80 +1.4 4,667,509 + srlR JDDGAC_24325 0.80 -0.9 4,667,510 - srlR JDDGAC_24325 0.80 -0.3 4,667,510 - srlR JDDGAC_24325 0.80 -1.9 4,667,510 - srlR JDDGAC_24325 0.80 +0.4 4,667,558 - srlR JDDGAC_24325 0.86 -0.9 4,667,558 - srlR JDDGAC_24325 0.86 -0.5 4,667,625 + +2.2 4,667,625 + -0.8 4,667,626 - -0.8 4,667,673 - -0.9 4,667,682 - +0.2 4,667,731 - -0.4 4,667,824 + gutM JDDGAC_24330 0.26 +0.6 4,667,878 + gutM JDDGAC_24330 0.41 -2.4
Or see this region's nucleotide sequence