Experiment: K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_06330 and tnpB are separated by 49 nucleotides tnpB and tnpB overlap by 4 nucleotides tnpB and tfaX are separated by 75 nucleotides tfaX and ybcY are separated by 56 nucleotides
JDDGAC_06330: JDDGAC_06330 - IS66-like element ISEc8 family transposase, at 1,107,512 to 1,109,050
_06330
JDDGAC_06335: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,109,100 to 1,109,447
tnpB
JDDGAC_06340: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,109,444 to 1,109,824
tnpB
JDDGAC_06345: tfaX - Protein TfaX, at 1,109,900 to 1,110,148
tfaX
JDDGAC_06350: ybcY - Putative uncharacterized protein YbcY, at 1,110,205 to 1,110,873
ybcY
Position (kb)
1109
1110
1111 Strain fitness (log2 ratio)
-1
0
1 at 1109.922 kb on - strand at 1109.922 kb on - strand at 1109.959 kb on + strand, within tfaX at 1110.058 kb on - strand, within tfaX at 1110.150 kb on + strand at 1110.261 kb on + strand at 1110.404 kb on - strand, within ybcY at 1110.416 kb on + strand, within ybcY at 1110.460 kb on - strand, within ybcY at 1110.606 kb on + strand, within ybcY at 1110.609 kb on - strand, within ybcY at 1110.629 kb on + strand, within ybcY at 1110.973 kb on - strand at 1110.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K30 remove 1,109,922 - -0.0 1,109,922 - -1.1 1,109,959 + tfaX JDDGAC_06345 0.24 -1.3 1,110,058 - tfaX JDDGAC_06345 0.63 -1.2 1,110,150 + -0.5 1,110,261 + -1.4 1,110,404 - ybcY JDDGAC_06350 0.30 +0.6 1,110,416 + ybcY JDDGAC_06350 0.32 +1.0 1,110,460 - ybcY JDDGAC_06350 0.38 +0.4 1,110,606 + ybcY JDDGAC_06350 0.60 -0.8 1,110,609 - ybcY JDDGAC_06350 0.60 +1.1 1,110,629 + ybcY JDDGAC_06350 0.63 -1.2 1,110,973 - +1.1 1,110,996 - +0.1
Or see this region's nucleotide sequence