Experiment: K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and wcaG are separated by 20 nucleotides wcaG and JDDGAC_04835 are separated by 290 nucleotides JDDGAC_04835 and ycjG overlap by 26 nucleotides
JDDGAC_04825: frsA - alpha/beta hydrolase, at 837,963 to 838,553
frsA
JDDGAC_04830: wcaG - NAD-dependent dehydratase, at 838,574 to 839,212
wcaG
JDDGAC_04835: JDDGAC_04835 - Murein peptide amidase A, at 839,503 to 840,231
_04835
JDDGAC_04840: ycjG - L-Ala-D/L-Glu epimerase, at 840,206 to 841,171
ycjG
Position (kb)
839
840
841 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 838.628 kb on - strand at 838.709 kb on - strand, within wcaG at 838.734 kb on + strand, within wcaG at 838.810 kb on - strand, within wcaG at 839.038 kb on + strand, within wcaG at 839.115 kb on + strand, within wcaG at 839.120 kb on - strand, within wcaG at 839.161 kb on + strand at 839.269 kb on - strand at 839.271 kb on + strand at 839.337 kb on + strand at 839.337 kb on + strand at 839.338 kb on - strand at 839.348 kb on - strand at 839.480 kb on + strand at 839.480 kb on + strand at 839.779 kb on - strand, within JDDGAC_04835 at 839.792 kb on + strand, within JDDGAC_04835 at 839.936 kb on + strand, within JDDGAC_04835 at 840.169 kb on + strand at 840.207 kb on + strand at 840.208 kb on - strand at 840.338 kb on - strand, within ycjG at 840.704 kb on - strand, within ycjG at 840.815 kb on - strand, within ycjG at 840.815 kb on - strand, within ycjG at 840.908 kb on - strand, within ycjG at 841.021 kb on - strand, within ycjG at 841.055 kb on - strand, within ycjG at 841.055 kb on - strand, within ycjG at 841.055 kb on - strand, within ycjG at 841.133 kb on + strand at 841.133 kb on + strand at 841.134 kb on - strand at 841.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K30 remove 838,628 - -0.2 838,709 - wcaG JDDGAC_04830 0.21 -0.1 838,734 + wcaG JDDGAC_04830 0.25 -1.0 838,810 - wcaG JDDGAC_04830 0.37 +0.0 839,038 + wcaG JDDGAC_04830 0.73 -0.2 839,115 + wcaG JDDGAC_04830 0.85 -0.5 839,120 - wcaG JDDGAC_04830 0.85 +0.6 839,161 + -1.7 839,269 - -0.1 839,271 + +0.4 839,337 + +0.4 839,337 + -1.3 839,338 - -0.2 839,348 - +1.4 839,480 + +0.1 839,480 + +0.6 839,779 - JDDGAC_04835 0.38 -2.0 839,792 + JDDGAC_04835 0.40 +1.5 839,936 + JDDGAC_04835 0.59 -1.5 840,169 + -0.8 840,207 + +0.2 840,208 - +0.8 840,338 - ycjG JDDGAC_04840 0.14 +0.0 840,704 - ycjG JDDGAC_04840 0.52 +0.3 840,815 - ycjG JDDGAC_04840 0.63 -0.9 840,815 - ycjG JDDGAC_04840 0.63 -2.9 840,908 - ycjG JDDGAC_04840 0.73 +0.2 841,021 - ycjG JDDGAC_04840 0.84 -0.2 841,055 - ycjG JDDGAC_04840 0.88 -0.2 841,055 - ycjG JDDGAC_04840 0.88 +1.1 841,055 - ycjG JDDGAC_04840 0.88 -1.0 841,133 + -0.1 841,133 + -1.7 841,134 - -0.1 841,176 - -0.2
Or see this region's nucleotide sequence