Experiment: K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcR and hicB are separated by 78 nucleotides hicB and hicA are separated by 24 nucleotides hicA and yncJ are separated by 281 nucleotides yncJ and rlhA are separated by 91 nucleotides
JDDGAC_04070: ydcR - Uncharacterized HTH-type transcriptional regulator YdcR, at 712,058 to 713,464
ydcR
JDDGAC_04075: hicB - type II toxin-antitoxin system antitoxin HicB, at 713,543 to 713,980
hicB
JDDGAC_04080: hicA - type II toxin-antitoxin system mRNA interferase toxin HicA, at 714,005 to 714,181
hicA
JDDGAC_04085: yncJ - Uncharacterized protein YncJ, at 714,463 to 714,633
yncJ
JDDGAC_04090: rlhA - 23S rRNA 5-hydroxycytidine C2501 synthase, at 714,725 to 716,728
rlhA
Position (kb)
714
715 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 713.075 kb on + strand, within ydcR at 713.272 kb on - strand, within ydcR at 713.299 kb on + strand, within ydcR at 713.300 kb on - strand, within ydcR at 713.300 kb on - strand, within ydcR at 713.300 kb on - strand, within ydcR at 713.300 kb on - strand, within ydcR at 713.433 kb on - strand at 713.455 kb on - strand at 713.915 kb on - strand, within hicB at 713.915 kb on - strand, within hicB at 713.915 kb on - strand, within hicB at 714.037 kb on + strand, within hicA at 714.202 kb on + strand at 714.203 kb on - strand at 714.203 kb on - strand at 714.203 kb on - strand at 714.241 kb on + strand at 714.257 kb on - strand at 714.347 kb on + strand at 714.809 kb on + strand at 714.810 kb on - strand at 714.955 kb on + strand, within rlhA
Per-strain Table
Position Strand Gene LocusTag Fraction K30 remove 713,075 + ydcR JDDGAC_04070 0.72 +1.3 713,272 - ydcR JDDGAC_04070 0.86 -0.8 713,299 + ydcR JDDGAC_04070 0.88 -0.7 713,300 - ydcR JDDGAC_04070 0.88 -1.0 713,300 - ydcR JDDGAC_04070 0.88 -1.5 713,300 - ydcR JDDGAC_04070 0.88 -0.3 713,300 - ydcR JDDGAC_04070 0.88 -0.0 713,433 - -0.4 713,455 - +0.3 713,915 - hicB JDDGAC_04075 0.85 +0.9 713,915 - hicB JDDGAC_04075 0.85 +1.6 713,915 - hicB JDDGAC_04075 0.85 +0.7 714,037 + hicA JDDGAC_04080 0.18 -1.3 714,202 + -1.3 714,203 - +1.1 714,203 - +1.9 714,203 - -0.1 714,241 + -1.1 714,257 - +1.4 714,347 + +0.9 714,809 + -0.3 714,810 - -2.5 714,955 + rlhA JDDGAC_04090 0.11 +0.4
Or see this region's nucleotide sequence