Experiment: K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadK and ydiT are separated by 56 nucleotides ydiT and ydiS overlap by 4 nucleotides ydiS and ydiR are separated by 55 nucleotides
JDDGAC_01725: fadK - medium-chain fatty-acid--CoA ligase, at 304,849 to 306,495
fadK
JDDGAC_01730: ydiT - Ferredoxin-like protein YdiT, at 306,552 to 306,845
ydiT
JDDGAC_01735: ydiS - putative electron transfer flavoprotein-quinone oxidoreductase YdiS, at 306,842 to 308,131
ydiS
JDDGAC_01740: ydiR - electron transfer flavoprotein subunit alpha, at 308,187 to 309,125
ydiR
Position (kb) 306
307
308
309 Strain fitness (log2 ratio) -2
-1
0
1
2 at 305.872 kb on - strand, within fadK at 305.910 kb on + strand, within fadK at 305.926 kb on + strand, within fadK at 305.926 kb on + strand, within fadK at 306.043 kb on + strand, within fadK at 306.076 kb on + strand, within fadK at 306.138 kb on + strand, within fadK at 306.139 kb on - strand, within fadK at 306.139 kb on - strand, within fadK at 306.142 kb on - strand, within fadK at 306.142 kb on - strand, within fadK at 306.222 kb on - strand, within fadK at 306.262 kb on + strand, within fadK at 306.339 kb on - strand at 306.344 kb on + strand at 306.345 kb on - strand at 306.481 kb on + strand at 306.572 kb on + strand at 306.612 kb on - strand, within ydiT at 306.696 kb on - strand, within ydiT at 306.768 kb on - strand, within ydiT at 306.862 kb on + strand at 307.052 kb on - strand, within ydiS at 307.451 kb on - strand, within ydiS at 307.526 kb on - strand, within ydiS at 307.528 kb on + strand, within ydiS at 307.590 kb on + strand, within ydiS at 307.685 kb on - strand, within ydiS at 307.811 kb on - strand, within ydiS at 307.823 kb on - strand, within ydiS at 307.824 kb on + strand, within ydiS at 308.228 kb on - strand at 308.258 kb on - strand at 308.328 kb on - strand, within ydiR at 308.328 kb on - strand, within ydiR at 308.328 kb on - strand, within ydiR at 308.402 kb on - strand, within ydiR at 308.402 kb on - strand, within ydiR at 308.402 kb on - strand, within ydiR at 308.402 kb on - strand, within ydiR at 308.620 kb on + strand, within ydiR at 308.621 kb on - strand, within ydiR at 308.625 kb on + strand, within ydiR at 308.654 kb on + strand, within ydiR at 308.747 kb on - strand, within ydiR at 308.973 kb on - strand, within ydiR at 308.987 kb on - strand, within ydiR at 309.032 kb on - strand at 309.032 kb on - strand at 309.077 kb on - strand at 309.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K30 remove 305,872 - fadK JDDGAC_01725 0.62 -0.2 305,910 + fadK JDDGAC_01725 0.64 -2.1 305,926 + fadK JDDGAC_01725 0.65 -1.7 305,926 + fadK JDDGAC_01725 0.65 -0.2 306,043 + fadK JDDGAC_01725 0.72 -0.5 306,076 + fadK JDDGAC_01725 0.74 +1.0 306,138 + fadK JDDGAC_01725 0.78 -0.4 306,139 - fadK JDDGAC_01725 0.78 -1.1 306,139 - fadK JDDGAC_01725 0.78 +0.7 306,142 - fadK JDDGAC_01725 0.79 +1.2 306,142 - fadK JDDGAC_01725 0.79 -0.2 306,222 - fadK JDDGAC_01725 0.83 +0.5 306,262 + fadK JDDGAC_01725 0.86 -0.4 306,339 - +0.8 306,344 + -2.6 306,345 - +1.0 306,481 + -0.5 306,572 + +1.0 306,612 - ydiT JDDGAC_01730 0.20 +0.2 306,696 - ydiT JDDGAC_01730 0.49 -1.5 306,768 - ydiT JDDGAC_01730 0.73 -0.1 306,862 + -0.6 307,052 - ydiS JDDGAC_01735 0.16 -0.8 307,451 - ydiS JDDGAC_01735 0.47 +0.6 307,526 - ydiS JDDGAC_01735 0.53 -0.0 307,528 + ydiS JDDGAC_01735 0.53 -0.0 307,590 + ydiS JDDGAC_01735 0.58 -1.2 307,685 - ydiS JDDGAC_01735 0.65 -1.4 307,811 - ydiS JDDGAC_01735 0.75 -0.5 307,823 - ydiS JDDGAC_01735 0.76 -1.2 307,824 + ydiS JDDGAC_01735 0.76 +1.2 308,228 - +0.8 308,258 - +0.7 308,328 - ydiR JDDGAC_01740 0.15 -1.7 308,328 - ydiR JDDGAC_01740 0.15 +0.4 308,328 - ydiR JDDGAC_01740 0.15 -2.0 308,402 - ydiR JDDGAC_01740 0.23 -0.0 308,402 - ydiR JDDGAC_01740 0.23 +1.5 308,402 - ydiR JDDGAC_01740 0.23 -1.1 308,402 - ydiR JDDGAC_01740 0.23 +1.5 308,620 + ydiR JDDGAC_01740 0.46 -0.6 308,621 - ydiR JDDGAC_01740 0.46 -1.0 308,625 + ydiR JDDGAC_01740 0.47 +0.3 308,654 + ydiR JDDGAC_01740 0.50 -1.7 308,747 - ydiR JDDGAC_01740 0.60 +0.5 308,973 - ydiR JDDGAC_01740 0.84 -1.0 308,987 - ydiR JDDGAC_01740 0.85 +0.2 309,032 - +1.2 309,032 - +0.2 309,077 - -0.7 309,114 + +2.6
Or see this region's nucleotide sequence