Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13050 and LRK53_RS13055 are separated by 26 nucleotides LRK53_RS13055 and LRK53_RS13060 are separated by 29 nucleotides LRK53_RS13060 and LRK53_RS13065 are separated by 200 nucleotides LRK53_RS13065 and LRK53_RS13070 are separated by 23 nucleotides LRK53_RS13070 and LRK53_RS13075 are separated by 544 nucleotides
LRK53_RS13050: LRK53_RS13050 - hypothetical protein, at 2,881,889 to 2,882,200
_RS13050
LRK53_RS13055: LRK53_RS13055 - hypothetical protein, at 2,882,227 to 2,882,580
_RS13055
LRK53_RS13060: LRK53_RS13060 - DksA/TraR family C4-type zinc finger protein, at 2,882,610 to 2,882,876
_RS13060
LRK53_RS13065: LRK53_RS13065 - acyl-CoA dehydrogenase family protein, at 2,883,077 to 2,884,846
_RS13065
LRK53_RS13070: LRK53_RS13070 - OsmC family protein, at 2,884,870 to 2,885,283
_RS13070
LRK53_RS13075: LRK53_RS13075 - cytochrome oxidase, at 2,885,828 to 2,886,376
_RS13075
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2882.148 kb on + strand, within LRK53_RS13050 at 2882.148 kb on + strand, within LRK53_RS13050 at 2882.149 kb on - strand, within LRK53_RS13050 at 2882.149 kb on - strand, within LRK53_RS13050 at 2882.149 kb on - strand, within LRK53_RS13050 at 2882.188 kb on + strand at 2882.270 kb on - strand, within LRK53_RS13055 at 2882.459 kb on - strand, within LRK53_RS13055 at 2882.459 kb on - strand, within LRK53_RS13055 at 2882.465 kb on + strand, within LRK53_RS13055 at 2882.465 kb on + strand, within LRK53_RS13055 at 2882.466 kb on - strand, within LRK53_RS13055 at 2882.466 kb on - strand, within LRK53_RS13055 at 2882.466 kb on - strand, within LRK53_RS13055 at 2882.466 kb on - strand, within LRK53_RS13055 at 2882.466 kb on - strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.494 kb on + strand, within LRK53_RS13055 at 2882.495 kb on - strand, within LRK53_RS13055 at 2882.495 kb on - strand, within LRK53_RS13055 at 2882.513 kb on - strand, within LRK53_RS13055 at 2882.549 kb on + strand at 2882.647 kb on + strand, within LRK53_RS13060 at 2882.647 kb on + strand, within LRK53_RS13060 at 2882.648 kb on - strand, within LRK53_RS13060 at 2882.648 kb on - strand, within LRK53_RS13060 at 2882.648 kb on - strand, within LRK53_RS13060 at 2882.882 kb on + strand at 2882.882 kb on + strand at 2882.883 kb on - strand at 2882.902 kb on + strand at 2882.902 kb on + strand at 2882.903 kb on - strand at 2882.903 kb on - strand at 2882.903 kb on - strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.909 kb on + strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2882.910 kb on - strand at 2883.186 kb on - strand at 2883.281 kb on + strand, within LRK53_RS13065 at 2883.281 kb on + strand, within LRK53_RS13065 at 2883.282 kb on - strand, within LRK53_RS13065 at 2883.282 kb on - strand, within LRK53_RS13065 at 2883.282 kb on - strand, within LRK53_RS13065 at 2883.282 kb on - strand, within LRK53_RS13065 at 2883.396 kb on - strand, within LRK53_RS13065 at 2883.518 kb on + strand, within LRK53_RS13065 at 2883.518 kb on + strand, within LRK53_RS13065 at 2883.519 kb on - strand, within LRK53_RS13065 at 2883.519 kb on - strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.689 kb on + strand, within LRK53_RS13065 at 2883.690 kb on - strand, within LRK53_RS13065 at 2883.690 kb on - strand, within LRK53_RS13065 at 2883.690 kb on - strand, within LRK53_RS13065 at 2883.690 kb on - strand, within LRK53_RS13065 at 2883.690 kb on - strand, within LRK53_RS13065 at 2884.124 kb on + strand, within LRK53_RS13065 at 2884.124 kb on + strand, within LRK53_RS13065 at 2884.125 kb on - strand, within LRK53_RS13065 at 2884.125 kb on - strand, within LRK53_RS13065 at 2884.184 kb on + strand, within LRK53_RS13065 at 2884.184 kb on + strand, within LRK53_RS13065 at 2884.185 kb on - strand, within LRK53_RS13065 at 2884.185 kb on - strand, within LRK53_RS13065 at 2884.185 kb on - strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.304 kb on + strand, within LRK53_RS13065 at 2884.305 kb on - strand, within LRK53_RS13065 at 2884.305 kb on - strand, within LRK53_RS13065 at 2884.305 kb on - strand, within LRK53_RS13065 at 2884.305 kb on - strand, within LRK53_RS13065 at 2884.415 kb on + strand, within LRK53_RS13065 at 2884.415 kb on + strand, within LRK53_RS13065 at 2884.416 kb on - strand, within LRK53_RS13065 at 2884.416 kb on - strand, within LRK53_RS13065 at 2884.416 kb on - strand, within LRK53_RS13065 at 2884.416 kb on - strand, within LRK53_RS13065 at 2884.416 kb on - strand, within LRK53_RS13065 at 2884.508 kb on + strand, within LRK53_RS13065 at 2884.509 kb on - strand, within LRK53_RS13065 at 2884.509 kb on - strand, within LRK53_RS13065 at 2884.509 kb on - strand, within LRK53_RS13065 at 2884.517 kb on + strand, within LRK53_RS13065 at 2884.517 kb on + strand, within LRK53_RS13065 at 2884.517 kb on + strand, within LRK53_RS13065 at 2884.517 kb on + strand, within LRK53_RS13065 at 2884.517 kb on + strand, within LRK53_RS13065 at 2884.518 kb on - strand, within LRK53_RS13065 at 2884.518 kb on - strand, within LRK53_RS13065 at 2884.518 kb on - strand, within LRK53_RS13065 at 2884.518 kb on - strand, within LRK53_RS13065 at 2884.518 kb on - strand, within LRK53_RS13065 at 2884.632 kb on + strand, within LRK53_RS13065 at 2884.633 kb on - strand, within LRK53_RS13065 at 2884.633 kb on - strand, within LRK53_RS13065 at 2884.633 kb on - strand, within LRK53_RS13065 at 2884.716 kb on + strand at 2884.716 kb on + strand at 2884.716 kb on + strand at 2884.716 kb on + strand at 2884.716 kb on + strand at 2884.717 kb on - strand at 2884.717 kb on - strand at 2884.717 kb on - strand at 2884.755 kb on + strand at 2884.756 kb on - strand at 2884.756 kb on - strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.826 kb on + strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2884.827 kb on - strand at 2885.056 kb on + strand, within LRK53_RS13070 at 2885.056 kb on + strand, within LRK53_RS13070 at 2885.056 kb on + strand, within LRK53_RS13070 at 2885.056 kb on + strand, within LRK53_RS13070 at 2885.056 kb on + strand, within LRK53_RS13070 at 2885.057 kb on - strand, within LRK53_RS13070 at 2885.057 kb on - strand, within LRK53_RS13070 at 2885.057 kb on - strand, within LRK53_RS13070 at 2885.057 kb on - strand, within LRK53_RS13070 at 2885.057 kb on - strand, within LRK53_RS13070 at 2885.292 kb on + strand at 2885.382 kb on + strand at 2885.382 kb on + strand at 2885.382 kb on + strand at 2885.382 kb on + strand at 2885.382 kb on + strand at 2885.382 kb on + strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.383 kb on - strand at 2885.715 kb on + strand at 2885.715 kb on + strand at 2885.715 kb on + strand at 2885.716 kb on - strand at 2885.788 kb on + strand at 2885.789 kb on - strand at 2885.789 kb on - strand at 2885.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 2,882,148 + LRK53_RS13050 0.83 -1.3 2,882,148 + LRK53_RS13050 0.83 -1.2 2,882,149 - LRK53_RS13050 0.83 -1.7 2,882,149 - LRK53_RS13050 0.83 -2.3 2,882,149 - LRK53_RS13050 0.83 +0.5 2,882,188 + +0.2 2,882,270 - LRK53_RS13055 0.12 -0.2 2,882,459 - LRK53_RS13055 0.66 -1.1 2,882,459 - LRK53_RS13055 0.66 -0.7 2,882,465 + LRK53_RS13055 0.67 -1.3 2,882,465 + LRK53_RS13055 0.67 -0.1 2,882,466 - LRK53_RS13055 0.68 -1.7 2,882,466 - LRK53_RS13055 0.68 +0.3 2,882,466 - LRK53_RS13055 0.68 -0.7 2,882,466 - LRK53_RS13055 0.68 -0.7 2,882,466 - LRK53_RS13055 0.68 -0.7 2,882,494 + LRK53_RS13055 0.75 -0.7 2,882,494 + LRK53_RS13055 0.75 -2.3 2,882,494 + LRK53_RS13055 0.75 -2.8 2,882,494 + LRK53_RS13055 0.75 -0.7 2,882,494 + LRK53_RS13055 0.75 -1.9 2,882,494 + LRK53_RS13055 0.75 -1.1 2,882,495 - LRK53_RS13055 0.76 -0.9 2,882,495 - LRK53_RS13055 0.76 -0.7 2,882,513 - LRK53_RS13055 0.81 -0.7 2,882,549 + -0.1 2,882,647 + LRK53_RS13060 0.14 -1.7 2,882,647 + LRK53_RS13060 0.14 -2.6 2,882,648 - LRK53_RS13060 0.14 -0.1 2,882,648 - LRK53_RS13060 0.14 -1.6 2,882,648 - LRK53_RS13060 0.14 -1.3 2,882,882 + -0.7 2,882,882 + -1.7 2,882,883 - -0.7 2,882,902 + +0.3 2,882,902 + -0.4 2,882,903 - -0.4 2,882,903 - -1.4 2,882,903 - -0.4 2,882,909 + -0.9 2,882,909 + -1.1 2,882,909 + -1.3 2,882,909 + -1.4 2,882,909 + -0.7 2,882,909 + -1.3 2,882,909 + -0.4 2,882,909 + -2.1 2,882,909 + -1.4 2,882,909 + -0.7 2,882,909 + -0.7 2,882,910 - -0.7 2,882,910 - +0.9 2,882,910 - -1.7 2,882,910 - -0.7 2,882,910 - -0.7 2,882,910 - +0.1 2,882,910 - -0.1 2,882,910 - -0.1 2,882,910 - -0.4 2,883,186 - -0.7 2,883,281 + LRK53_RS13065 0.12 -0.7 2,883,281 + LRK53_RS13065 0.12 -1.4 2,883,282 - LRK53_RS13065 0.12 -1.7 2,883,282 - LRK53_RS13065 0.12 -1.4 2,883,282 - LRK53_RS13065 0.12 +0.9 2,883,282 - LRK53_RS13065 0.12 -1.9 2,883,396 - LRK53_RS13065 0.18 +0.1 2,883,518 + LRK53_RS13065 0.25 +0.6 2,883,518 + LRK53_RS13065 0.25 -1.9 2,883,519 - LRK53_RS13065 0.25 +0.9 2,883,519 - LRK53_RS13065 0.25 -0.9 2,883,689 + LRK53_RS13065 0.35 +0.1 2,883,689 + LRK53_RS13065 0.35 -0.1 2,883,689 + LRK53_RS13065 0.35 +0.9 2,883,689 + LRK53_RS13065 0.35 -1.4 2,883,689 + LRK53_RS13065 0.35 -0.1 2,883,689 + LRK53_RS13065 0.35 -0.7 2,883,689 + LRK53_RS13065 0.35 -0.6 2,883,689 + LRK53_RS13065 0.35 -0.1 2,883,689 + LRK53_RS13065 0.35 -1.9 2,883,689 + LRK53_RS13065 0.35 +0.9 2,883,690 - LRK53_RS13065 0.35 +0.9 2,883,690 - LRK53_RS13065 0.35 -1.4 2,883,690 - LRK53_RS13065 0.35 -1.9 2,883,690 - LRK53_RS13065 0.35 +0.1 2,883,690 - LRK53_RS13065 0.35 -0.8 2,884,124 + LRK53_RS13065 0.59 -0.1 2,884,124 + LRK53_RS13065 0.59 -1.4 2,884,125 - LRK53_RS13065 0.59 -2.9 2,884,125 - LRK53_RS13065 0.59 -2.1 2,884,184 + LRK53_RS13065 0.63 -1.1 2,884,184 + LRK53_RS13065 0.63 -0.9 2,884,185 - LRK53_RS13065 0.63 -0.9 2,884,185 - LRK53_RS13065 0.63 -1.1 2,884,185 - LRK53_RS13065 0.63 -2.7 2,884,304 + LRK53_RS13065 0.69 +0.9 2,884,304 + LRK53_RS13065 0.69 -2.1 2,884,304 + LRK53_RS13065 0.69 -1.9 2,884,304 + LRK53_RS13065 0.69 -2.2 2,884,304 + LRK53_RS13065 0.69 -0.1 2,884,304 + LRK53_RS13065 0.69 -0.7 2,884,305 - LRK53_RS13065 0.69 +0.3 2,884,305 - LRK53_RS13065 0.69 -0.3 2,884,305 - LRK53_RS13065 0.69 -0.1 2,884,305 - LRK53_RS13065 0.69 -0.7 2,884,415 + LRK53_RS13065 0.76 -1.1 2,884,415 + LRK53_RS13065 0.76 -0.9 2,884,416 - LRK53_RS13065 0.76 -0.7 2,884,416 - LRK53_RS13065 0.76 +1.9 2,884,416 - LRK53_RS13065 0.76 -0.2 2,884,416 - LRK53_RS13065 0.76 -0.7 2,884,416 - LRK53_RS13065 0.76 -1.1 2,884,508 + LRK53_RS13065 0.81 -0.4 2,884,509 - LRK53_RS13065 0.81 -2.3 2,884,509 - LRK53_RS13065 0.81 -1.4 2,884,509 - LRK53_RS13065 0.81 +0.9 2,884,517 + LRK53_RS13065 0.81 -2.7 2,884,517 + LRK53_RS13065 0.81 -1.4 2,884,517 + LRK53_RS13065 0.81 -0.6 2,884,517 + LRK53_RS13065 0.81 +0.5 2,884,517 + LRK53_RS13065 0.81 -0.7 2,884,518 - LRK53_RS13065 0.81 -0.7 2,884,518 - LRK53_RS13065 0.81 -1.1 2,884,518 - LRK53_RS13065 0.81 -0.7 2,884,518 - LRK53_RS13065 0.81 -1.0 2,884,518 - LRK53_RS13065 0.81 +1.6 2,884,632 + LRK53_RS13065 0.88 +0.3 2,884,633 - LRK53_RS13065 0.88 -0.7 2,884,633 - LRK53_RS13065 0.88 -0.1 2,884,633 - LRK53_RS13065 0.88 -0.1 2,884,716 + -1.3 2,884,716 + -1.1 2,884,716 + -1.4 2,884,716 + -1.4 2,884,716 + -1.4 2,884,717 - -0.8 2,884,717 - +0.1 2,884,717 - -1.6 2,884,755 + -2.2 2,884,756 - +0.9 2,884,756 - -1.1 2,884,826 + -2.2 2,884,826 + -0.1 2,884,826 + +0.9 2,884,826 + -0.3 2,884,826 + -0.7 2,884,826 + +0.3 2,884,826 + +1.9 2,884,827 - -1.7 2,884,827 - -0.7 2,884,827 - -2.3 2,884,827 - -1.2 2,884,827 - -1.4 2,884,827 - +0.9 2,884,827 - -1.7 2,884,827 - +0.3 2,884,827 - -0.9 2,884,827 - +0.1 2,884,827 - +1.5 2,884,827 - -1.3 2,885,056 + LRK53_RS13070 0.45 -1.1 2,885,056 + LRK53_RS13070 0.45 -1.1 2,885,056 + LRK53_RS13070 0.45 -0.6 2,885,056 + LRK53_RS13070 0.45 -1.8 2,885,056 + LRK53_RS13070 0.45 +0.9 2,885,057 - LRK53_RS13070 0.45 +0.9 2,885,057 - LRK53_RS13070 0.45 -0.1 2,885,057 - LRK53_RS13070 0.45 +0.1 2,885,057 - LRK53_RS13070 0.45 -0.7 2,885,057 - LRK53_RS13070 0.45 -0.1 2,885,292 + -0.6 2,885,382 + -0.4 2,885,382 + -0.7 2,885,382 + -0.8 2,885,382 + -1.1 2,885,382 + -1.0 2,885,382 + +1.9 2,885,383 - -0.6 2,885,383 - -1.1 2,885,383 - +0.9 2,885,383 - -1.1 2,885,383 - -0.1 2,885,383 - +0.0 2,885,383 - -2.1 2,885,715 + +0.5 2,885,715 + +0.2 2,885,715 + -1.9 2,885,716 - -0.7 2,885,788 + +0.7 2,885,789 - -0.7 2,885,789 - +1.5 2,885,789 - -2.3
Or see this region's nucleotide sequence