Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11480 and LRK53_RS11485 are separated by 286 nucleotides LRK53_RS11485 and LRK53_RS11490 are separated by 11 nucleotides LRK53_RS11490 and LRK53_RS11495 are separated by 7 nucleotides LRK53_RS11495 and LRK53_RS11500 are separated by 125 nucleotides LRK53_RS11500 and LRK53_RS11505 are separated by 59 nucleotides
LRK53_RS11480: LRK53_RS11480 - hypothetical protein, at 2,556,404 to 2,556,649
_RS11480
LRK53_RS11485: LRK53_RS11485 - tRNA-Leu, at 2,556,936 to 2,557,020
_RS11485
LRK53_RS11490: LRK53_RS11490 - preprotein translocase subunit SecG, at 2,557,032 to 2,557,493
_RS11490
LRK53_RS11495: LRK53_RS11495 - triose-phosphate isomerase, at 2,557,501 to 2,558,268
_RS11495
LRK53_RS11500: LRK53_RS11500 - SDR family oxidoreductase, at 2,558,394 to 2,559,203
_RS11500
LRK53_RS11505: LRK53_RS11505 - DUF6445 family protein, at 2,559,263 to 2,559,937
_RS11505
Position (kb)
2557
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2556.701 kb on + strand at 2556.701 kb on + strand at 2556.702 kb on - strand at 2556.744 kb on + strand at 2556.745 kb on - strand at 2556.745 kb on - strand at 2556.745 kb on - strand at 2556.745 kb on - strand at 2556.760 kb on + strand at 2556.760 kb on + strand at 2556.761 kb on - strand at 2556.761 kb on - strand at 2556.761 kb on - strand at 2556.761 kb on - strand at 2556.771 kb on + strand at 2556.800 kb on + strand at 2556.800 kb on + strand at 2556.800 kb on + strand at 2556.800 kb on + strand at 2556.800 kb on + strand at 2556.800 kb on + strand at 2556.801 kb on - strand at 2556.801 kb on - strand at 2556.801 kb on - strand at 2556.801 kb on - strand at 2556.801 kb on - strand at 2556.902 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.934 kb on + strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2556.935 kb on - strand at 2557.066 kb on - strand at 2557.066 kb on - strand at 2557.066 kb on - strand at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.277 kb on - strand, within LRK53_RS11490 at 2557.503 kb on - strand at 2558.670 kb on + strand, within LRK53_RS11500 at 2558.684 kb on + strand, within LRK53_RS11500 at 2558.685 kb on - strand, within LRK53_RS11500 at 2558.833 kb on + strand, within LRK53_RS11500 at 2558.833 kb on + strand, within LRK53_RS11500 at 2558.834 kb on - strand, within LRK53_RS11500 at 2558.859 kb on + strand, within LRK53_RS11500 at 2558.859 kb on + strand, within LRK53_RS11500 at 2558.859 kb on + strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.860 kb on - strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.877 kb on + strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2558.878 kb on - strand, within LRK53_RS11500 at 2559.089 kb on + strand, within LRK53_RS11500 at 2559.089 kb on + strand, within LRK53_RS11500 at 2559.089 kb on + strand, within LRK53_RS11500 at 2559.090 kb on - strand, within LRK53_RS11500 at 2559.090 kb on - strand, within LRK53_RS11500 at 2559.090 kb on - strand, within LRK53_RS11500 at 2559.090 kb on - strand, within LRK53_RS11500 at 2559.201 kb on + strand at 2559.201 kb on + strand at 2559.201 kb on + strand at 2559.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 2,556,701 + -1.7 2,556,701 + -0.8 2,556,702 - +0.8 2,556,744 + -2.3 2,556,745 - +1.3 2,556,745 - +0.8 2,556,745 - -2.6 2,556,745 - -0.5 2,556,760 + -2.5 2,556,760 + -0.2 2,556,761 - -1.3 2,556,761 - -0.5 2,556,761 - +0.8 2,556,761 - -0.2 2,556,771 + -0.8 2,556,800 + -3.0 2,556,800 + -0.2 2,556,800 + -1.2 2,556,800 + -0.8 2,556,800 + -1.5 2,556,800 + +0.8 2,556,801 - +0.4 2,556,801 - -0.7 2,556,801 - -1.0 2,556,801 - -1.0 2,556,801 - -1.2 2,556,902 + -0.2 2,556,934 + +0.8 2,556,934 + +1.0 2,556,934 + -1.3 2,556,934 + -0.8 2,556,934 + +0.4 2,556,934 + -1.9 2,556,934 + -0.3 2,556,934 + +1.8 2,556,934 + -1.2 2,556,934 + -0.6 2,556,934 + -0.2 2,556,934 + -0.9 2,556,935 - -0.8 2,556,935 - -2.2 2,556,935 - -2.2 2,556,935 - +1.2 2,556,935 - -0.2 2,556,935 - -0.8 2,556,935 - -1.1 2,556,935 - -0.4 2,556,935 - +0.1 2,556,935 - -1.2 2,557,066 - +0.8 2,557,066 - -1.2 2,557,066 - -2.0 2,557,277 - LRK53_RS11490 0.53 +0.1 2,557,277 - LRK53_RS11490 0.53 -0.4 2,557,277 - LRK53_RS11490 0.53 +1.8 2,557,277 - LRK53_RS11490 0.53 +0.8 2,557,277 - LRK53_RS11490 0.53 +1.8 2,557,277 - LRK53_RS11490 0.53 -0.4 2,557,277 - LRK53_RS11490 0.53 -0.6 2,557,277 - LRK53_RS11490 0.53 -2.0 2,557,277 - LRK53_RS11490 0.53 +1.8 2,557,277 - LRK53_RS11490 0.53 +0.4 2,557,277 - LRK53_RS11490 0.53 -0.8 2,557,277 - LRK53_RS11490 0.53 +0.8 2,557,277 - LRK53_RS11490 0.53 +0.2 2,557,277 - LRK53_RS11490 0.53 -1.1 2,557,277 - LRK53_RS11490 0.53 -0.2 2,557,277 - LRK53_RS11490 0.53 -0.3 2,557,503 - -1.8 2,558,670 + LRK53_RS11500 0.34 -0.9 2,558,684 + LRK53_RS11500 0.36 -1.2 2,558,685 - LRK53_RS11500 0.36 -1.6 2,558,833 + LRK53_RS11500 0.54 -1.0 2,558,833 + LRK53_RS11500 0.54 -0.8 2,558,834 - LRK53_RS11500 0.54 +0.4 2,558,859 + LRK53_RS11500 0.57 +0.8 2,558,859 + LRK53_RS11500 0.57 -0.2 2,558,859 + LRK53_RS11500 0.57 -0.1 2,558,860 - LRK53_RS11500 0.58 -2.0 2,558,860 - LRK53_RS11500 0.58 -0.8 2,558,860 - LRK53_RS11500 0.58 +0.2 2,558,860 - LRK53_RS11500 0.58 +0.8 2,558,860 - LRK53_RS11500 0.58 -1.2 2,558,860 - LRK53_RS11500 0.58 -0.8 2,558,860 - LRK53_RS11500 0.58 +0.2 2,558,860 - LRK53_RS11500 0.58 -1.2 2,558,860 - LRK53_RS11500 0.58 +0.8 2,558,877 + LRK53_RS11500 0.60 +0.8 2,558,877 + LRK53_RS11500 0.60 +1.4 2,558,877 + LRK53_RS11500 0.60 -1.4 2,558,877 + LRK53_RS11500 0.60 -1.8 2,558,877 + LRK53_RS11500 0.60 -0.2 2,558,877 + LRK53_RS11500 0.60 -2.0 2,558,878 - LRK53_RS11500 0.60 +0.8 2,558,878 - LRK53_RS11500 0.60 +0.3 2,558,878 - LRK53_RS11500 0.60 -1.2 2,558,878 - LRK53_RS11500 0.60 -1.2 2,558,878 - LRK53_RS11500 0.60 -0.8 2,558,878 - LRK53_RS11500 0.60 -0.5 2,559,089 + LRK53_RS11500 0.86 -2.2 2,559,089 + LRK53_RS11500 0.86 -0.2 2,559,089 + LRK53_RS11500 0.86 -1.5 2,559,090 - LRK53_RS11500 0.86 -2.0 2,559,090 - LRK53_RS11500 0.86 +0.2 2,559,090 - LRK53_RS11500 0.86 -1.1 2,559,090 - LRK53_RS11500 0.86 -1.5 2,559,201 + -1.5 2,559,201 + +0.2 2,559,201 + -2.5 2,559,202 - -1.5
Or see this region's nucleotide sequence