Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09440 and LRK53_RS09445 are separated by 122 nucleotides LRK53_RS09445 and LRK53_RS09450 are separated by 130 nucleotides LRK53_RS09450 and LRK53_RS09455 are separated by 182 nucleotides
LRK53_RS09440: LRK53_RS09440 - propionate--CoA ligase, at 2,116,921 to 2,118,813
_RS09440
LRK53_RS09445: LRK53_RS09445 - alkyl hydroperoxide reductase subunit F, at 2,118,936 to 2,120,522
_RS09445
LRK53_RS09450: LRK53_RS09450 - alkyl hydroperoxide reductase subunit C, at 2,120,653 to 2,121,216
_RS09450
LRK53_RS09455: LRK53_RS09455 - dicarboxylate/amino acid:cation symporter, at 2,121,399 to 2,122,715
_RS09455
Position (kb)
2118
2119
2120
2121 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.974 kb on + strand, within LRK53_RS09440 at 2117.975 kb on - strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.079 kb on + strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.080 kb on - strand, within LRK53_RS09440 at 2118.316 kb on + strand, within LRK53_RS09440 at 2118.316 kb on + strand, within LRK53_RS09440 at 2118.316 kb on + strand, within LRK53_RS09440 at 2118.316 kb on + strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.317 kb on - strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.343 kb on + strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.344 kb on - strand, within LRK53_RS09440 at 2118.382 kb on + strand, within LRK53_RS09440 at 2118.382 kb on + strand, within LRK53_RS09440 at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.649 kb on + strand at 2118.650 kb on - strand at 2118.650 kb on - strand at 2118.650 kb on - strand at 2118.650 kb on - strand at 2118.819 kb on + strand at 2118.820 kb on - strand at 2119.042 kb on + strand at 2119.042 kb on + strand at 2119.042 kb on + strand at 2119.042 kb on + strand at 2119.042 kb on + strand at 2119.043 kb on - strand at 2119.043 kb on - strand at 2119.043 kb on - strand at 2119.043 kb on - strand at 2119.072 kb on - strand at 2119.226 kb on + strand, within LRK53_RS09445 at 2119.238 kb on - strand, within LRK53_RS09445 at 2119.238 kb on - strand, within LRK53_RS09445 at 2119.238 kb on - strand, within LRK53_RS09445 at 2119.238 kb on - strand, within LRK53_RS09445 at 2119.238 kb on - strand, within LRK53_RS09445 at 2119.492 kb on + strand, within LRK53_RS09445 at 2119.492 kb on + strand, within LRK53_RS09445 at 2119.492 kb on + strand, within LRK53_RS09445 at 2119.493 kb on - strand, within LRK53_RS09445 at 2119.493 kb on - strand, within LRK53_RS09445 at 2119.493 kb on - strand, within LRK53_RS09445 at 2119.514 kb on - strand, within LRK53_RS09445 at 2119.514 kb on - strand, within LRK53_RS09445 at 2119.687 kb on + strand, within LRK53_RS09445 at 2119.738 kb on + strand, within LRK53_RS09445 at 2119.738 kb on + strand, within LRK53_RS09445 at 2119.780 kb on - strand, within LRK53_RS09445 at 2119.780 kb on - strand, within LRK53_RS09445 at 2119.837 kb on + strand, within LRK53_RS09445 at 2119.837 kb on + strand, within LRK53_RS09445 at 2119.837 kb on + strand, within LRK53_RS09445 at 2119.837 kb on + strand, within LRK53_RS09445 at 2119.838 kb on - strand, within LRK53_RS09445 at 2119.838 kb on - strand, within LRK53_RS09445 at 2119.838 kb on - strand, within LRK53_RS09445 at 2119.838 kb on - strand, within LRK53_RS09445 at 2120.002 kb on + strand, within LRK53_RS09445 at 2120.051 kb on - strand, within LRK53_RS09445 at 2120.051 kb on - strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.070 kb on + strand, within LRK53_RS09445 at 2120.071 kb on - strand, within LRK53_RS09445 at 2120.071 kb on - strand, within LRK53_RS09445 at 2120.071 kb on - strand, within LRK53_RS09445 at 2120.071 kb on - strand, within LRK53_RS09445 at 2120.071 kb on - strand, within LRK53_RS09445 at 2120.155 kb on + strand, within LRK53_RS09445 at 2120.155 kb on + strand, within LRK53_RS09445 at 2120.155 kb on + strand, within LRK53_RS09445 at 2120.155 kb on + strand, within LRK53_RS09445 at 2120.155 kb on + strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.156 kb on - strand, within LRK53_RS09445 at 2120.170 kb on + strand, within LRK53_RS09445 at 2120.190 kb on + strand, within LRK53_RS09445 at 2120.191 kb on - strand, within LRK53_RS09445 at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.485 kb on + strand at 2120.486 kb on - strand at 2120.486 kb on - strand at 2120.486 kb on - strand at 2120.486 kb on - strand at 2120.552 kb on + strand at 2120.553 kb on - strand at 2120.586 kb on - strand at 2120.586 kb on - strand at 2120.654 kb on + strand at 2120.654 kb on + strand at 2120.654 kb on + strand at 2121.045 kb on - strand, within LRK53_RS09450 at 2121.045 kb on - strand, within LRK53_RS09450 at 2121.258 kb on + strand at 2121.258 kb on + strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.259 kb on - strand at 2121.281 kb on + strand at 2121.313 kb on + strand at 2121.314 kb on - strand at 2121.314 kb on - strand at 2121.318 kb on - strand at 2121.331 kb on + strand at 2121.331 kb on + strand at 2121.331 kb on + strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.332 kb on - strand at 2121.400 kb on + strand at 2121.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 2,117,974 + LRK53_RS09440 0.56 +0.0 2,117,974 + LRK53_RS09440 0.56 +0.0 2,117,974 + LRK53_RS09440 0.56 +2.0 2,117,974 + LRK53_RS09440 0.56 +0.0 2,117,974 + LRK53_RS09440 0.56 +0.8 2,117,974 + LRK53_RS09440 0.56 +0.6 2,117,974 + LRK53_RS09440 0.56 -0.9 2,117,975 - LRK53_RS09440 0.56 -0.7 2,118,079 + LRK53_RS09440 0.61 -0.6 2,118,079 + LRK53_RS09440 0.61 -0.3 2,118,079 + LRK53_RS09440 0.61 +0.4 2,118,079 + LRK53_RS09440 0.61 -1.0 2,118,079 + LRK53_RS09440 0.61 -1.6 2,118,079 + LRK53_RS09440 0.61 -0.3 2,118,079 + LRK53_RS09440 0.61 -1.0 2,118,079 + LRK53_RS09440 0.61 +1.0 2,118,079 + LRK53_RS09440 0.61 +0.4 2,118,079 + LRK53_RS09440 0.61 -0.6 2,118,079 + LRK53_RS09440 0.61 +0.6 2,118,079 + LRK53_RS09440 0.61 -0.6 2,118,079 + LRK53_RS09440 0.61 +0.4 2,118,079 + LRK53_RS09440 0.61 +0.0 2,118,079 + LRK53_RS09440 0.61 -1.3 2,118,079 + LRK53_RS09440 0.61 +1.0 2,118,080 - LRK53_RS09440 0.61 +0.0 2,118,080 - LRK53_RS09440 0.61 -1.0 2,118,080 - LRK53_RS09440 0.61 +0.4 2,118,080 - LRK53_RS09440 0.61 +1.0 2,118,080 - LRK53_RS09440 0.61 +1.0 2,118,080 - LRK53_RS09440 0.61 -1.0 2,118,080 - LRK53_RS09440 0.61 +0.4 2,118,080 - LRK53_RS09440 0.61 +2.6 2,118,080 - LRK53_RS09440 0.61 +0.4 2,118,080 - LRK53_RS09440 0.61 +1.6 2,118,316 + LRK53_RS09440 0.74 -1.6 2,118,316 + LRK53_RS09440 0.74 -0.6 2,118,316 + LRK53_RS09440 0.74 +1.0 2,118,316 + LRK53_RS09440 0.74 +1.0 2,118,317 - LRK53_RS09440 0.74 -0.5 2,118,317 - LRK53_RS09440 0.74 -1.0 2,118,317 - LRK53_RS09440 0.74 -1.0 2,118,317 - LRK53_RS09440 0.74 -0.7 2,118,317 - LRK53_RS09440 0.74 -0.7 2,118,317 - LRK53_RS09440 0.74 +1.0 2,118,317 - LRK53_RS09440 0.74 +0.0 2,118,343 + LRK53_RS09440 0.75 -1.8 2,118,343 + LRK53_RS09440 0.75 -1.8 2,118,343 + LRK53_RS09440 0.75 -0.7 2,118,343 + LRK53_RS09440 0.75 -1.0 2,118,343 + LRK53_RS09440 0.75 -2.0 2,118,343 + LRK53_RS09440 0.75 +0.0 2,118,343 + LRK53_RS09440 0.75 +0.4 2,118,343 + LRK53_RS09440 0.75 -1.4 2,118,344 - LRK53_RS09440 0.75 -0.2 2,118,344 - LRK53_RS09440 0.75 +0.0 2,118,344 - LRK53_RS09440 0.75 +1.6 2,118,344 - LRK53_RS09440 0.75 -1.0 2,118,344 - LRK53_RS09440 0.75 +1.0 2,118,344 - LRK53_RS09440 0.75 -2.0 2,118,344 - LRK53_RS09440 0.75 +0.0 2,118,344 - LRK53_RS09440 0.75 -0.9 2,118,344 - LRK53_RS09440 0.75 -0.1 2,118,344 - LRK53_RS09440 0.75 -1.0 2,118,344 - LRK53_RS09440 0.75 -0.2 2,118,344 - LRK53_RS09440 0.75 +0.4 2,118,382 + LRK53_RS09440 0.77 -0.1 2,118,382 + LRK53_RS09440 0.77 -0.9 2,118,649 + +0.3 2,118,649 + +1.0 2,118,649 + -0.6 2,118,649 + -0.3 2,118,649 + -0.1 2,118,649 + -0.7 2,118,649 + -0.2 2,118,649 + -1.3 2,118,649 + +0.2 2,118,650 - -0.5 2,118,650 - -1.0 2,118,650 - -0.4 2,118,650 - +0.4 2,118,819 + -0.6 2,118,820 - -1.3 2,119,042 + -1.9 2,119,042 + -0.9 2,119,042 + -0.6 2,119,042 + -0.6 2,119,042 + -2.3 2,119,043 - +0.8 2,119,043 - -0.6 2,119,043 - +0.4 2,119,043 - +0.7 2,119,072 - -0.8 2,119,226 + LRK53_RS09445 0.18 +0.0 2,119,238 - LRK53_RS09445 0.19 -2.0 2,119,238 - LRK53_RS09445 0.19 +1.3 2,119,238 - LRK53_RS09445 0.19 -1.3 2,119,238 - LRK53_RS09445 0.19 +1.0 2,119,238 - LRK53_RS09445 0.19 +0.0 2,119,492 + LRK53_RS09445 0.35 -0.6 2,119,492 + LRK53_RS09445 0.35 -2.3 2,119,492 + LRK53_RS09445 0.35 -1.1 2,119,493 - LRK53_RS09445 0.35 -0.6 2,119,493 - LRK53_RS09445 0.35 -1.6 2,119,493 - LRK53_RS09445 0.35 -2.1 2,119,514 - LRK53_RS09445 0.36 -1.3 2,119,514 - LRK53_RS09445 0.36 +0.0 2,119,687 + LRK53_RS09445 0.47 -2.1 2,119,738 + LRK53_RS09445 0.51 +0.0 2,119,738 + LRK53_RS09445 0.51 -1.8 2,119,780 - LRK53_RS09445 0.53 -0.2 2,119,780 - LRK53_RS09445 0.53 -0.2 2,119,837 + LRK53_RS09445 0.57 -1.0 2,119,837 + LRK53_RS09445 0.57 +0.0 2,119,837 + LRK53_RS09445 0.57 +1.0 2,119,837 + LRK53_RS09445 0.57 +1.0 2,119,838 - LRK53_RS09445 0.57 -1.3 2,119,838 - LRK53_RS09445 0.57 -1.2 2,119,838 - LRK53_RS09445 0.57 -1.6 2,119,838 - LRK53_RS09445 0.57 +0.0 2,120,002 + LRK53_RS09445 0.67 +0.2 2,120,051 - LRK53_RS09445 0.70 -1.3 2,120,051 - LRK53_RS09445 0.70 -0.6 2,120,070 + LRK53_RS09445 0.71 -1.6 2,120,070 + LRK53_RS09445 0.71 +1.6 2,120,070 + LRK53_RS09445 0.71 +0.5 2,120,070 + LRK53_RS09445 0.71 -0.6 2,120,070 + LRK53_RS09445 0.71 +1.0 2,120,070 + LRK53_RS09445 0.71 -1.8 2,120,071 - LRK53_RS09445 0.72 +0.4 2,120,071 - LRK53_RS09445 0.72 -1.1 2,120,071 - LRK53_RS09445 0.72 -0.3 2,120,071 - LRK53_RS09445 0.72 -1.0 2,120,071 - LRK53_RS09445 0.72 -1.0 2,120,155 + LRK53_RS09445 0.77 +0.0 2,120,155 + LRK53_RS09445 0.77 -1.2 2,120,155 + LRK53_RS09445 0.77 +1.8 2,120,155 + LRK53_RS09445 0.77 -1.3 2,120,155 + LRK53_RS09445 0.77 +1.0 2,120,156 - LRK53_RS09445 0.77 +0.0 2,120,156 - LRK53_RS09445 0.77 -1.9 2,120,156 - LRK53_RS09445 0.77 -2.1 2,120,156 - LRK53_RS09445 0.77 -0.6 2,120,156 - LRK53_RS09445 0.77 -1.4 2,120,156 - LRK53_RS09445 0.77 -1.7 2,120,156 - LRK53_RS09445 0.77 +0.3 2,120,170 + LRK53_RS09445 0.78 -0.3 2,120,190 + LRK53_RS09445 0.79 +1.6 2,120,191 - LRK53_RS09445 0.79 +2.0 2,120,485 + -0.2 2,120,485 + -0.7 2,120,485 + -1.0 2,120,485 + +0.2 2,120,485 + -0.6 2,120,485 + +1.0 2,120,486 - -1.6 2,120,486 - -1.7 2,120,486 - -1.2 2,120,486 - -0.1 2,120,552 + +2.0 2,120,553 - -1.0 2,120,586 - -1.6 2,120,586 - +0.0 2,120,654 + -1.2 2,120,654 + -0.6 2,120,654 + -1.0 2,121,045 - LRK53_RS09450 0.70 +0.0 2,121,045 - LRK53_RS09450 0.70 +0.0 2,121,258 + +1.0 2,121,258 + -1.0 2,121,259 - -1.8 2,121,259 - +2.0 2,121,259 - -1.3 2,121,259 - +0.3 2,121,281 + +0.0 2,121,313 + +2.0 2,121,314 - +0.0 2,121,314 - -1.8 2,121,318 - +0.3 2,121,331 + -2.7 2,121,331 + +0.0 2,121,331 + +1.0 2,121,332 - -0.6 2,121,332 - -0.7 2,121,332 - -0.3 2,121,332 - -1.1 2,121,332 - +0.0 2,121,332 - -0.6 2,121,332 - +0.0 2,121,332 - -1.8 2,121,400 + -1.3 2,121,434 - -1.0
Or see this region's nucleotide sequence