Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07420 and LRK53_RS07425 are separated by 32 nucleotides LRK53_RS07425 and LRK53_RS07430 are separated by 96 nucleotides
LRK53_RS07420: LRK53_RS07420 - DNA repair protein RadA, at 1,660,831 to 1,662,216
_RS07420
LRK53_RS07425: LRK53_RS07425 - cytochrome c biogenesis protein CcsA, at 1,662,249 to 1,663,049
_RS07425
LRK53_RS07430: LRK53_RS07430 - signal recognition particle protein, at 1,663,146 to 1,664,525
_RS07430
Position (kb)
1662
1663
1664 Strain fitness (log2 ratio)
-2
-1
0
1 at 1661.426 kb on + strand, within LRK53_RS07420 at 1661.426 kb on + strand, within LRK53_RS07420 at 1661.426 kb on + strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.560 kb on + strand, within LRK53_RS07420 at 1661.560 kb on + strand, within LRK53_RS07420 at 1661.560 kb on + strand, within LRK53_RS07420 at 1661.561 kb on - strand, within LRK53_RS07420 at 1661.896 kb on + strand, within LRK53_RS07420 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1663.009 kb on + strand at 1663.010 kb on - strand at 1663.010 kb on - strand at 1663.111 kb on + strand at 1663.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 1,661,426 + LRK53_RS07420 0.43 +0.8 1,661,426 + LRK53_RS07420 0.43 +0.8 1,661,426 + LRK53_RS07420 0.43 -1.8 1,661,427 - LRK53_RS07420 0.43 -0.8 1,661,427 - LRK53_RS07420 0.43 -0.8 1,661,427 - LRK53_RS07420 0.43 -0.8 1,661,560 + LRK53_RS07420 0.53 -0.8 1,661,560 + LRK53_RS07420 0.53 -0.5 1,661,560 + LRK53_RS07420 0.53 -0.8 1,661,561 - LRK53_RS07420 0.53 -0.8 1,661,896 + LRK53_RS07420 0.77 -1.0 1,662,382 + LRK53_RS07425 0.17 +0.8 1,662,382 + LRK53_RS07425 0.17 -0.6 1,662,382 + LRK53_RS07425 0.17 -0.3 1,662,382 + LRK53_RS07425 0.17 +0.1 1,662,383 - LRK53_RS07425 0.17 +0.5 1,662,383 - LRK53_RS07425 0.17 -1.8 1,662,383 - LRK53_RS07425 0.17 -0.3 1,662,383 - LRK53_RS07425 0.17 -1.2 1,662,383 - LRK53_RS07425 0.17 +0.4 1,663,009 + +0.8 1,663,010 - -1.1 1,663,010 - -2.0 1,663,111 + -0.3 1,663,112 - -0.5
Or see this region's nucleotide sequence