Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04180 and LRK53_RS04185 are separated by 227 nucleotides LRK53_RS04185 and LRK53_RS04190 are separated by 15 nucleotides
LRK53_RS04180: LRK53_RS04180 - ABC transporter ATP-binding protein, at 977,164 to 978,048
_RS04180
LRK53_RS04185: LRK53_RS04185 - dihydrolipoyllysine-residue acetyltransferase, at 978,276 to 979,691
_RS04185
LRK53_RS04190: LRK53_RS04190 - dihydrolipoyl dehydrogenase, at 979,707 to 981,521
_RS04190
Position (kb) 978
979
980 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.730 kb on + strand, within LRK53_RS04180 at 977.731 kb on - strand, within LRK53_RS04180 at 977.787 kb on + strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.792 kb on + strand, within LRK53_RS04180 at 977.792 kb on + strand, within LRK53_RS04180 at 977.793 kb on - strand, within LRK53_RS04180 at 978.017 kb on + strand at 978.017 kb on + strand at 978.018 kb on - strand at 978.018 kb on - strand at 978.018 kb on - strand at 978.037 kb on + strand at 978.037 kb on + strand at 978.038 kb on - strand at 978.038 kb on - strand at 978.038 kb on - strand at 978.096 kb on - strand at 978.138 kb on + strand at 978.138 kb on + strand at 978.139 kb on - strand at 978.139 kb on - strand at 978.139 kb on - strand at 978.211 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.252 kb on - strand at 978.500 kb on + strand, within LRK53_RS04185 at 978.501 kb on - strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.504 kb on + strand, within LRK53_RS04185 at 978.518 kb on + strand, within LRK53_RS04185 at 978.518 kb on + strand, within LRK53_RS04185 at 978.786 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.599 kb on + strand at 979.600 kb on - strand at 979.600 kb on - strand at 980.157 kb on + strand, within LRK53_RS04190 at 980.214 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 977,648 + LRK53_RS04180 0.55 -0.9 977,648 + LRK53_RS04180 0.55 +0.8 977,648 + LRK53_RS04180 0.55 -0.1 977,648 + LRK53_RS04180 0.55 -0.1 977,648 + LRK53_RS04180 0.55 -0.4 977,649 - LRK53_RS04180 0.55 -0.4 977,649 - LRK53_RS04180 0.55 +0.9 977,649 - LRK53_RS04180 0.55 +2.2 977,649 - LRK53_RS04180 0.55 +0.2 977,649 - LRK53_RS04180 0.55 -1.1 977,730 + LRK53_RS04180 0.64 -0.4 977,731 - LRK53_RS04180 0.64 +0.3 977,787 + LRK53_RS04180 0.70 -1.7 977,788 - LRK53_RS04180 0.71 -0.1 977,788 - LRK53_RS04180 0.71 -0.1 977,788 - LRK53_RS04180 0.71 -0.5 977,792 + LRK53_RS04180 0.71 -0.9 977,792 + LRK53_RS04180 0.71 +0.9 977,793 - LRK53_RS04180 0.71 +1.3 978,017 + -2.3 978,017 + -0.4 978,018 - -0.7 978,018 - -1.0 978,018 - -0.4 978,037 + -1.7 978,037 + -0.1 978,038 - -1.8 978,038 - -1.1 978,038 - -0.9 978,096 - -1.1 978,138 + -1.3 978,138 + -1.1 978,139 - -1.1 978,139 - -0.7 978,139 - +0.9 978,211 + -0.5 978,227 + -0.4 978,227 + -1.7 978,227 + +1.9 978,227 + -1.4 978,227 + +0.3 978,227 + -0.1 978,227 + -1.3 978,227 + -1.4 978,227 + -1.1 978,227 + -0.7 978,227 + +0.9 978,227 + -1.4 978,228 - +0.9 978,228 - -2.1 978,228 - -0.7 978,228 - +0.9 978,228 - -0.7 978,228 - -3.8 978,228 - -1.4 978,228 - -2.1 978,252 - -0.7 978,500 + LRK53_RS04185 0.16 -0.1 978,501 - LRK53_RS04185 0.16 +0.9 978,502 + LRK53_RS04185 0.16 -0.1 978,502 + LRK53_RS04185 0.16 -2.8 978,502 + LRK53_RS04185 0.16 -0.7 978,503 - LRK53_RS04185 0.16 -1.1 978,503 - LRK53_RS04185 0.16 -1.4 978,503 - LRK53_RS04185 0.16 -0.1 978,503 - LRK53_RS04185 0.16 -0.4 978,503 - LRK53_RS04185 0.16 -0.1 978,503 - LRK53_RS04185 0.16 -2.1 978,504 + LRK53_RS04185 0.16 -0.7 978,518 + LRK53_RS04185 0.17 -1.1 978,518 + LRK53_RS04185 0.17 -2.9 978,786 + LRK53_RS04185 0.36 -1.1 978,894 + LRK53_RS04185 0.44 -2.3 978,894 + LRK53_RS04185 0.44 -1.1 978,894 + LRK53_RS04185 0.44 -1.7 978,894 + LRK53_RS04185 0.44 -0.7 978,894 + LRK53_RS04185 0.44 -1.1 978,894 + LRK53_RS04185 0.44 -1.1 978,894 + LRK53_RS04185 0.44 +0.9 978,894 + LRK53_RS04185 0.44 -1.1 978,894 + LRK53_RS04185 0.44 -2.4 978,895 - LRK53_RS04185 0.44 -0.4 978,895 - LRK53_RS04185 0.44 +0.9 978,895 - LRK53_RS04185 0.44 -2.1 978,895 - LRK53_RS04185 0.44 -2.1 978,895 - LRK53_RS04185 0.44 -0.1 978,895 - LRK53_RS04185 0.44 -0.7 979,476 + LRK53_RS04185 0.85 -0.1 979,476 + LRK53_RS04185 0.85 -0.3 979,476 + LRK53_RS04185 0.85 +0.9 979,476 + LRK53_RS04185 0.85 -2.7 979,476 + LRK53_RS04185 0.85 -2.1 979,477 - LRK53_RS04185 0.85 +0.9 979,477 - LRK53_RS04185 0.85 -0.7 979,477 - LRK53_RS04185 0.85 -0.1 979,599 + -3.0 979,600 - -0.1 979,600 - -1.8 980,157 + LRK53_RS04190 0.25 -1.5 980,214 + LRK53_RS04190 0.28 -1.7 980,682 + LRK53_RS04190 0.54 -1.1 980,682 + LRK53_RS04190 0.54 -1.4 980,682 + LRK53_RS04190 0.54 -0.1 980,683 - LRK53_RS04190 0.54 -2.3 980,683 - LRK53_RS04190 0.54 -1.1 980,683 - LRK53_RS04190 0.54 +0.9
Or see this region's nucleotide sequence