Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18985 and LRK53_RS18990 are separated by 82 nucleotides LRK53_RS18990 and LRK53_RS18995 are separated by 85 nucleotides LRK53_RS18995 and LRK53_RS19000 are separated by 117 nucleotides LRK53_RS19000 and LRK53_RS19005 are separated by 85 nucleotides
LRK53_RS18985: LRK53_RS18985 - hypothetical protein, at 189,254 to 189,619
_RS18985
LRK53_RS18990: LRK53_RS18990 - hypothetical protein, at 189,702 to 190,061
_RS18990
LRK53_RS18995: LRK53_RS18995 - hypothetical protein, at 190,147 to 190,500
_RS18995
LRK53_RS19000: LRK53_RS19000 - hypothetical protein, at 190,618 to 190,953
_RS19000
LRK53_RS19005: LRK53_RS19005 - phosphoadenosine phosphosulfate reductase family protein, at 191,039 to 192,148
_RS19005
Position (kb) 190
191 Strain fitness (log2 ratio) -2
-1
0
1
2 at 189.148 kb on - strand at 189.148 kb on - strand at 189.396 kb on + strand, within LRK53_RS18985 at 189.396 kb on + strand, within LRK53_RS18985 at 189.396 kb on + strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.397 kb on - strand, within LRK53_RS18985 at 189.518 kb on + strand, within LRK53_RS18985 at 189.518 kb on + strand, within LRK53_RS18985 at 189.519 kb on - strand, within LRK53_RS18985 at 189.519 kb on - strand, within LRK53_RS18985 at 189.519 kb on - strand, within LRK53_RS18985 at 189.519 kb on - strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.521 kb on + strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.522 kb on - strand, within LRK53_RS18985 at 189.753 kb on + strand, within LRK53_RS18990 at 189.753 kb on + strand, within LRK53_RS18990 at 189.753 kb on + strand, within LRK53_RS18990 at 189.753 kb on + strand, within LRK53_RS18990 at 189.754 kb on - strand, within LRK53_RS18990 at 189.754 kb on - strand, within LRK53_RS18990 at 189.756 kb on + strand, within LRK53_RS18990 at 189.756 kb on + strand, within LRK53_RS18990 at 189.756 kb on + strand, within LRK53_RS18990 at 189.756 kb on + strand, within LRK53_RS18990 at 189.757 kb on - strand, within LRK53_RS18990 at 189.757 kb on - strand, within LRK53_RS18990 at 189.757 kb on - strand, within LRK53_RS18990 at 189.757 kb on - strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.921 kb on + strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.922 kb on - strand, within LRK53_RS18990 at 189.966 kb on + strand, within LRK53_RS18990 at 189.966 kb on + strand, within LRK53_RS18990 at 189.967 kb on - strand, within LRK53_RS18990 at 189.967 kb on - strand, within LRK53_RS18990 at 189.982 kb on - strand, within LRK53_RS18990 at 189.982 kb on - strand, within LRK53_RS18990 at 189.982 kb on - strand, within LRK53_RS18990 at 189.982 kb on - strand, within LRK53_RS18990 at 189.982 kb on - strand, within LRK53_RS18990 at 190.059 kb on + strand at 190.059 kb on + strand at 190.059 kb on + strand at 190.059 kb on + strand at 190.059 kb on + strand at 190.060 kb on - strand at 190.060 kb on - strand at 190.060 kb on - strand at 190.060 kb on - strand at 190.110 kb on + strand at 190.111 kb on - strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.114 kb on + strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.115 kb on - strand at 190.210 kb on + strand, within LRK53_RS18995 at 190.210 kb on + strand, within LRK53_RS18995 at 190.295 kb on + strand, within LRK53_RS18995 at 190.295 kb on + strand, within LRK53_RS18995 at 190.412 kb on - strand, within LRK53_RS18995 at 190.517 kb on + strand at 190.517 kb on + strand at 190.517 kb on + strand at 190.664 kb on + strand, within LRK53_RS19000 at 190.665 kb on - strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.882 kb on + strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.883 kb on - strand, within LRK53_RS19000 at 190.884 kb on + strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.885 kb on - strand, within LRK53_RS19000 at 190.951 kb on + strand at 190.952 kb on - strand at 190.952 kb on - strand at 190.952 kb on - strand at 191.001 kb on - strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.038 kb on + strand at 191.039 kb on - strand at 191.039 kb on - strand at 191.039 kb on - strand at 191.039 kb on - strand at 191.039 kb on - strand at 191.039 kb on - strand at 191.363 kb on + strand, within LRK53_RS19005 at 191.363 kb on + strand, within LRK53_RS19005 at 191.363 kb on + strand, within LRK53_RS19005 at 191.363 kb on + strand, within LRK53_RS19005 at 191.364 kb on - strand, within LRK53_RS19005 at 191.364 kb on - strand, within LRK53_RS19005 at 191.451 kb on + strand, within LRK53_RS19005 at 191.451 kb on + strand, within LRK53_RS19005 at 191.452 kb on - strand, within LRK53_RS19005
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 189,148 - +0.3 189,148 - -0.7 189,396 + LRK53_RS18985 0.39 +0.6 189,396 + LRK53_RS18985 0.39 -1.2 189,396 + LRK53_RS18985 0.39 -0.4 189,397 - LRK53_RS18985 0.39 -0.3 189,397 - LRK53_RS18985 0.39 +0.6 189,397 - LRK53_RS18985 0.39 +0.2 189,397 - LRK53_RS18985 0.39 -0.4 189,397 - LRK53_RS18985 0.39 +0.6 189,397 - LRK53_RS18985 0.39 -0.5 189,518 + LRK53_RS18985 0.72 -0.2 189,518 + LRK53_RS18985 0.72 +0.0 189,519 - LRK53_RS18985 0.72 +0.6 189,519 - LRK53_RS18985 0.72 -0.2 189,519 - LRK53_RS18985 0.72 +0.6 189,519 - LRK53_RS18985 0.72 -0.4 189,521 + LRK53_RS18985 0.73 -0.7 189,521 + LRK53_RS18985 0.73 +0.3 189,521 + LRK53_RS18985 0.73 +0.5 189,521 + LRK53_RS18985 0.73 -1.4 189,521 + LRK53_RS18985 0.73 +0.9 189,521 + LRK53_RS18985 0.73 -1.4 189,521 + LRK53_RS18985 0.73 +0.6 189,521 + LRK53_RS18985 0.73 -0.7 189,521 + LRK53_RS18985 0.73 -1.9 189,521 + LRK53_RS18985 0.73 -0.9 189,522 - LRK53_RS18985 0.73 +0.9 189,522 - LRK53_RS18985 0.73 -1.7 189,522 - LRK53_RS18985 0.73 +0.6 189,522 - LRK53_RS18985 0.73 -1.1 189,522 - LRK53_RS18985 0.73 -1.6 189,522 - LRK53_RS18985 0.73 -1.6 189,522 - LRK53_RS18985 0.73 -0.1 189,522 - LRK53_RS18985 0.73 -0.2 189,522 - LRK53_RS18985 0.73 +0.6 189,522 - LRK53_RS18985 0.73 -0.9 189,522 - LRK53_RS18985 0.73 -0.7 189,522 - LRK53_RS18985 0.73 +0.6 189,522 - LRK53_RS18985 0.73 -0.2 189,522 - LRK53_RS18985 0.73 +0.0 189,753 + LRK53_RS18990 0.14 -0.2 189,753 + LRK53_RS18990 0.14 -0.4 189,753 + LRK53_RS18990 0.14 -1.0 189,753 + LRK53_RS18990 0.14 -0.9 189,754 - LRK53_RS18990 0.14 -0.1 189,754 - LRK53_RS18990 0.14 +0.2 189,756 + LRK53_RS18990 0.15 -0.4 189,756 + LRK53_RS18990 0.15 +1.6 189,756 + LRK53_RS18990 0.15 -1.0 189,756 + LRK53_RS18990 0.15 -1.4 189,757 - LRK53_RS18990 0.15 -0.4 189,757 - LRK53_RS18990 0.15 -1.2 189,757 - LRK53_RS18990 0.15 +0.4 189,757 - LRK53_RS18990 0.15 +0.6 189,921 + LRK53_RS18990 0.61 -0.5 189,921 + LRK53_RS18990 0.61 -1.0 189,921 + LRK53_RS18990 0.61 +1.6 189,921 + LRK53_RS18990 0.61 -1.4 189,921 + LRK53_RS18990 0.61 -0.7 189,921 + LRK53_RS18990 0.61 +0.0 189,921 + LRK53_RS18990 0.61 -0.5 189,921 + LRK53_RS18990 0.61 +2.6 189,921 + LRK53_RS18990 0.61 -0.7 189,922 - LRK53_RS18990 0.61 +0.3 189,922 - LRK53_RS18990 0.61 -1.0 189,922 - LRK53_RS18990 0.61 +1.6 189,922 - LRK53_RS18990 0.61 -1.0 189,922 - LRK53_RS18990 0.61 -2.1 189,922 - LRK53_RS18990 0.61 -0.7 189,922 - LRK53_RS18990 0.61 -0.7 189,966 + LRK53_RS18990 0.73 -2.0 189,966 + LRK53_RS18990 0.73 +2.2 189,967 - LRK53_RS18990 0.74 +0.2 189,967 - LRK53_RS18990 0.74 +0.3 189,982 - LRK53_RS18990 0.78 -0.2 189,982 - LRK53_RS18990 0.78 +0.6 189,982 - LRK53_RS18990 0.78 -0.3 189,982 - LRK53_RS18990 0.78 -1.5 189,982 - LRK53_RS18990 0.78 -0.7 190,059 + -2.2 190,059 + +1.6 190,059 + -1.4 190,059 + -1.8 190,059 + -2.3 190,060 - +0.9 190,060 - -0.7 190,060 - -0.4 190,060 - +0.6 190,110 + +0.4 190,111 - +0.0 190,114 + +0.6 190,114 + -0.7 190,114 + -2.1 190,114 + +0.3 190,114 + -0.7 190,114 + +1.0 190,114 + +0.6 190,115 - -0.7 190,115 - -0.4 190,115 - +1.0 190,115 - -0.7 190,115 - -1.1 190,115 - +0.6 190,115 - +0.6 190,115 - -1.0 190,115 - -1.0 190,115 - +0.6 190,115 - -1.2 190,115 - +0.3 190,115 - -0.7 190,115 - +0.6 190,115 - -1.6 190,115 - -0.9 190,115 - +1.6 190,115 - -1.6 190,115 - -0.7 190,115 - +0.9 190,210 + LRK53_RS18995 0.18 -1.1 190,210 + LRK53_RS18995 0.18 -0.8 190,295 + LRK53_RS18995 0.42 -1.0 190,295 + LRK53_RS18995 0.42 -1.4 190,412 - LRK53_RS18995 0.75 -0.7 190,517 + +0.3 190,517 + +0.6 190,517 + -0.4 190,664 + LRK53_RS19000 0.14 +0.6 190,665 - LRK53_RS19000 0.14 +0.0 190,882 + LRK53_RS19000 0.79 +0.0 190,882 + LRK53_RS19000 0.79 -0.9 190,882 + LRK53_RS19000 0.79 -0.4 190,882 + LRK53_RS19000 0.79 -1.0 190,882 + LRK53_RS19000 0.79 -0.2 190,882 + LRK53_RS19000 0.79 -1.4 190,882 + LRK53_RS19000 0.79 -0.4 190,882 + LRK53_RS19000 0.79 -1.0 190,883 - LRK53_RS19000 0.79 +1.6 190,883 - LRK53_RS19000 0.79 -1.5 190,883 - LRK53_RS19000 0.79 -1.1 190,883 - LRK53_RS19000 0.79 -0.2 190,883 - LRK53_RS19000 0.79 -0.8 190,883 - LRK53_RS19000 0.79 -0.6 190,883 - LRK53_RS19000 0.79 -0.4 190,883 - LRK53_RS19000 0.79 -0.7 190,884 + LRK53_RS19000 0.79 -0.4 190,885 - LRK53_RS19000 0.79 -0.4 190,885 - LRK53_RS19000 0.79 -0.7 190,885 - LRK53_RS19000 0.79 -0.4 190,885 - LRK53_RS19000 0.79 +0.0 190,885 - LRK53_RS19000 0.79 -1.0 190,885 - LRK53_RS19000 0.79 +0.6 190,951 + +0.6 190,952 - -1.0 190,952 - -0.7 190,952 - -0.2 191,001 - +1.6 191,038 + +0.6 191,038 + -0.2 191,038 + +0.4 191,038 + -0.6 191,038 + -1.2 191,038 + +0.0 191,038 + -1.0 191,038 + +0.6 191,039 - -1.4 191,039 - +0.0 191,039 - -0.2 191,039 - +0.1 191,039 - +0.0 191,039 - +0.3 191,363 + LRK53_RS19005 0.29 -1.2 191,363 + LRK53_RS19005 0.29 +0.9 191,363 + LRK53_RS19005 0.29 -2.1 191,363 + LRK53_RS19005 0.29 +1.0 191,364 - LRK53_RS19005 0.29 -1.4 191,364 - LRK53_RS19005 0.29 -1.7 191,451 + LRK53_RS19005 0.37 -0.4 191,451 + LRK53_RS19005 0.37 -0.4 191,452 - LRK53_RS19005 0.37 +0.3
Or see this region's nucleotide sequence