Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18145 and LRK53_RS18150 are separated by 14 nucleotides LRK53_RS18150 and LRK53_RS18155 overlap by 4 nucleotides
LRK53_RS18145: LRK53_RS18145 - DHHA1 domain-containing protein, at 2,971 to 4,749
_RS18145
LRK53_RS18150: LRK53_RS18150 - hypothetical protein, at 4,764 to 5,423
_RS18150
LRK53_RS18155: LRK53_RS18155 - hypothetical protein, at 5,420 to 6,601
_RS18155
Position (kb) 4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.844 kb on + strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 3.845 kb on - strand, within LRK53_RS18145 at 4.673 kb on - strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.759 kb on - strand at 4.759 kb on - strand at 4.759 kb on - strand at 4.759 kb on - strand at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.421 kb on + strand at 5.421 kb on + strand at 5.421 kb on + strand at 5.421 kb on + strand at 5.421 kb on + strand at 5.422 kb on - strand at 5.422 kb on - strand at 5.422 kb on - strand at 5.425 kb on + strand at 5.425 kb on + strand at 5.425 kb on + strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.622 kb on - strand, within LRK53_RS18155 at 5.979 kb on + strand, within LRK53_RS18155 at 6.036 kb on - strand, within LRK53_RS18155 at 6.179 kb on + strand, within LRK53_RS18155 at 6.334 kb on - strand, within LRK53_RS18155
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 3,844 + LRK53_RS18145 0.49 +0.6 3,844 + LRK53_RS18145 0.49 -0.8 3,844 + LRK53_RS18145 0.49 -0.6 3,844 + LRK53_RS18145 0.49 -0.4 3,844 + LRK53_RS18145 0.49 +0.6 3,844 + LRK53_RS18145 0.49 -1.0 3,844 + LRK53_RS18145 0.49 -0.4 3,845 - LRK53_RS18145 0.49 +0.3 3,845 - LRK53_RS18145 0.49 -1.5 3,845 - LRK53_RS18145 0.49 -0.8 3,845 - LRK53_RS18145 0.49 +0.6 3,845 - LRK53_RS18145 0.49 +0.3 3,845 - LRK53_RS18145 0.49 -0.7 3,845 - LRK53_RS18145 0.49 -0.7 3,845 - LRK53_RS18145 0.49 -2.0 3,845 - LRK53_RS18145 0.49 -2.4 3,845 - LRK53_RS18145 0.49 +0.6 3,845 - LRK53_RS18145 0.49 -2.8 3,845 - LRK53_RS18145 0.49 -1.8 4,673 - -1.0 4,758 + -0.2 4,758 + -1.6 4,758 + -1.0 4,758 + +2.4 4,758 + -0.4 4,758 + -0.4 4,759 - -0.4 4,759 - -1.2 4,759 - -0.7 4,759 - -0.5 5,197 + LRK53_RS18150 0.66 -1.2 5,197 + LRK53_RS18150 0.66 -0.4 5,197 + LRK53_RS18150 0.66 -1.3 5,197 + LRK53_RS18150 0.66 +1.6 5,197 + LRK53_RS18150 0.66 -0.5 5,197 + LRK53_RS18150 0.66 +0.6 5,197 + LRK53_RS18150 0.66 -2.3 5,197 + LRK53_RS18150 0.66 -1.4 5,197 + LRK53_RS18150 0.66 -0.7 5,197 + LRK53_RS18150 0.66 -0.8 5,197 + LRK53_RS18150 0.66 -0.0 5,197 + LRK53_RS18150 0.66 -2.0 5,197 + LRK53_RS18150 0.66 -1.6 5,197 + LRK53_RS18150 0.66 -0.0 5,197 + LRK53_RS18150 0.66 -0.9 5,198 - LRK53_RS18150 0.66 -1.9 5,198 - LRK53_RS18150 0.66 -0.4 5,198 - LRK53_RS18150 0.66 -0.6 5,198 - LRK53_RS18150 0.66 +0.6 5,198 - LRK53_RS18150 0.66 +0.5 5,198 - LRK53_RS18150 0.66 -1.8 5,198 - LRK53_RS18150 0.66 -1.3 5,198 - LRK53_RS18150 0.66 +0.6 5,198 - LRK53_RS18150 0.66 -0.7 5,198 - LRK53_RS18150 0.66 +0.3 5,198 - LRK53_RS18150 0.66 +0.6 5,198 - LRK53_RS18150 0.66 -0.7 5,421 + -1.0 5,421 + -0.7 5,421 + -2.3 5,421 + +1.6 5,421 + -1.1 5,422 - -0.4 5,422 - -1.6 5,422 - +0.6 5,425 + -0.6 5,425 + -0.4 5,425 + +1.6 5,426 - +1.6 5,426 - +1.0 5,426 - +0.6 5,426 - +0.3 5,426 - -0.0 5,426 - -0.7 5,622 - LRK53_RS18155 0.17 +0.6 5,979 + LRK53_RS18155 0.47 -0.6 6,036 - LRK53_RS18155 0.52 -1.7 6,179 + LRK53_RS18155 0.64 -0.0 6,334 - LRK53_RS18155 0.77 +1.1
Or see this region's nucleotide sequence